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Register a new userEffect of salt-stress (NaCl) on growth indicators and chlorophyll content of SO4 Grape rootstock propagated in vitro
تأثير الإجهاد الملحي (NaCl) في بعض مؤشرات النمو ومحتوى اليخضور في أصل العنب SO4 المستزرع مخبرياً
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درس تأثير الإجهاد الملحي باستخدام تراكيز مختلفة من كلوريد الصوديوم NaCl) 50 ،100 و 150 ميلي مول) في بعض الصفات الفيزيولوجية و مؤشرات النمو لأصـل العنـب SO4 المـستزرع نـسيجياً vitro in في الهيئة العامة للبحوث العلمية الزراعية بدمشق. أدت زيادة التركيـز الملحـي فـي وسـط الزراعة إلى انخفاض في مؤشرات النمو (نسبة النباتات المتبقية و متوسط طول النبـات و متوسـط عـدد العيون) بالمقارنة بنباتات الشاهد و ذلك بعد أربعة أسابيع من الزراعة على أوساط التكاثر التـي تحتـوي على التركيزين 50 ميلي مول و 100 ميلي مول من كلوريد الصوديوم، بينما تعرضت النباتـات المعاملـة 150 ميلي مول من كلوريد الصوديوم للموت، كما أدت الأوسـاط المغذيـة المحتويـة علـى التركيـزين 50 ميلي مول و 100 ميلي مول من كلوريد الصوديوم إلى انخفاض محتوى الأوراق من اليخضور الكلي.
In this study, the effect of different concentrations of sodium chloride (NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some growth indicators of SO4 grape rootstock propagated in vitro was studied at the laboratories of General Commission for Scientific Agricultural Research (GCSAR), Damascus/Syria. Results indicated that the increase of NaCl concentration up to 100 mM in culture medium led to reduce the growth indicators (survival plants, average of the plant length and average number of buds) with significant differences when compared with the control plants after 4 weeks from culturing on multiplication media, while treatment with 150 mM of sodium chloride led to death of all plants. Using the concentrations 50 and 100 mM of NaCl also caused decreasing the total chlorophyll content in the leaves.
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Research summary
تتناول هذه الدراسة تأثير الإجهاد الملحي باستخدام تراكيز مختلفة من كلوريد الصوديوم (NaCl) على بعض الخصائص الفيزيولوجية ومؤشرات النمو لأصل العنب SO4 المستزرع نسيجياً في المختبر. أظهرت النتائج أن زيادة تركيز NaCl حتى 100 ميلي مول في وسط الزراعة أدى إلى انخفاض مؤشرات النمو مثل نسبة النباتات المتبقية، متوسط طول النبات، ومتوسط عدد العيون مقارنة بالنباتات الشاهدة بعد 4 أسابيع من الزراعة. بينما أدى التركيز 150 ميلي مول من NaCl إلى موت جميع النباتات. كما أدى استخدام التراكيز 50 و100 ميلي مول من NaCl إلى انخفاض محتوى الأوراق من اليخضور الكلي.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة في مجال زراعة العنب وتحديد تأثير الإجهاد الملحي على نمو النبات. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الأفضل تضمين مزيد من التفاصيل حول كيفية تأثير الإجهاد الملحي على العمليات البيوكيميائية داخل النبات. ثانياً، يمكن توسيع نطاق الدراسة لتشمل أصناف أخرى من العنب لمعرفة ما إذا كانت النتائج متسقة عبر أنواع مختلفة. أخيراً، كان من الممكن استخدام تقنيات تحليلية أكثر تقدماً لقياس محتوى اليخضور والعناصر الأخرى في الأوراق.
Questions related to the research
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ما هو الهدف الرئيسي من الدراسة؟
الهدف الرئيسي هو دراسة تأثير الإجهاد الملحي على بعض الخصائص الفيزيولوجية ومؤشرات النمو لأصل العنب SO4 المستزرع نسيجياً.
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ما هي التراكيز المختلفة من NaCl التي تم استخدامها في الدراسة؟
تم استخدام تراكيز 0، 50، 100، و150 ميلي مول من NaCl.
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ما هي النتائج الرئيسية التي توصلت إليها الدراسة؟
أظهرت النتائج أن زيادة تركيز NaCl حتى 100 ميلي مول أدى إلى انخفاض مؤشرات النمو ومحتوى اليخضور، بينما أدى التركيز 150 ميلي مول إلى موت جميع النباتات.
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ما هي التوصيات المستقبلية التي يمكن استخلاصها من هذه الدراسة؟
يوصى بإجراء دراسات إضافية تشمل أصناف أخرى من العنب واستخدام تقنيات تحليلية متقدمة لفهم تأثير الإجهاد الملحي على العمليات البيوكيميائية داخل النبات.
References used
Alleweldt, G., P. Spiegel and B. Reisch. 1990. Grapes (Vitis), Acta Horticulturae, 290: 289-327
Al-Saidi, I And B. Alawi. 1984. Effect of different concentrations of NaCl and CaCl2 on growth, dry weight and mineral elements of some grapevine cultivars (Vitis vinifera), Annals-of-agricultural-Science, 29:2, 971-988
Anguiano, C., H.A. Altube and M.W. Borys. 1989. Growth and yields of Vitis vinifera L. cultivars Ribier and green Hungarian in normal and saline soils. Revista-Chapingo, 13-16:62-63, 173-176
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This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
This current study was carried out on B41 grape rootstock
micropropagated in vitro at the General Commission for Scientific Agricultural
Research (GCSAR), Syria to study the effect of different concentrations of
sodium chloride on the multiplicati
on and rooting stages. The results showed
that the highest average of survival plants (98%), the number of new buds
(8.43) and plant length (8.58 cm) were obtained in the control treatment after 4
weeks from multiplication stage. The treatment with 50 mM of sodium chloride
led to reduce the multiplication rates with significant differences, while the
lowest plant multiplication had occurred with 100 mM of sodium chloride and
no survival plants were remained by the treatment with 150 mM of sodium
chloride during multiplication stage. The highest rooting rates (% 85.30), the
number of roots (4.67) and root length (6.28 cm) were also obtained in the
control treatment after 4 weeks from rooting stage. The treatment with 50 mM
of sodium chloride led to reduce the rooting process with significant
differences. The lowest rooting results were observed with 100 mM of sodium
chloride and no rooting rate was observed when plants were treated with 150
mM of sodium chloride.
The study showed that IAA treated cuttings were rooted earlier and bud
development was delayed. On the other hand, IAA treatment increased rooting
percentage and reduced the percentage of bud development. Increasing
NaCl salinity to ٠,١ M decrease
d the rooting ability in cuttings, while the
use of IAA could mitigate the adverse effect of salinity. The best results of
rooting were obtained in treatment of (٠,٠٥ M).
Despite being one of the reasons of production decline, salinity is still a tool
to improve production quality in some hybrids. this research was conducted at
the Faculty of Agriculture, University of Damascus during 2012 season. A salt
stress was
applied on two tomato hybrids, Bonaparte and Marmara by
increasing the value of the electrical conductivity of irrigating water from 4 to
13 ds / m, at the stage of the third true leaf to the end of growth of the two
tomato plants.
Results indicated that salt stress led to a reduction in the fruit weight of
both hybrids but it helped in improving some chemical and physical
characteristics relating to fruit quality, such as increasing the fruit content of
soluble solids in both hybrids. Salt stress did not affect the lycopene content of
fruits in Bonapart, but it was increased by 2 folds inMarmara. Some fruit color
indices (a* and b*) were greater in Bonaparte while L* indice was higher in
Marmara. However, the color indices of fruits were not affected by salinity in
both hybrids studied.
This study was conducted at the laboratories of Horticultural Department, Faculty
of Agriculture, Damascus University to determine the effect of using three levels of
sucrose (10, 30, 50 g /l) on chlorophyll content between healthy and vitrified pl
ants of
three varieties of Gypsophylla panicolata (Golantwo, Planshnet, Newlove). Results
showed superiority of treatment 10 g / l sucrose in reducing vitrification percent to
36.78% compared with 62.57% for control (30 g/l) and with significant differences
between varieties. Planshnet was the best variety in chlorophyll content either in healthy
or in vitrified plantlets. Moreover, the treatment with sucrose of 10 g/l caused significant
increment of chlorophyll content compared with other treatments. The high total
chlorophyll content was 17.46 micrograms/ ml in healthy plants compared with 14.26
micrograms / ml in vitrification plants for Planshnet at 10 g/l sucrose.
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