In this study, the effect of different concentrations of sodium chloride
(NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some
growth indicators of SO4 grape rootstock propagated in vitro was studied at the
laboratories of Gen
eral Commission for Scientific Agricultural Research
(GCSAR), Damascus/Syria. Results indicated that the increase of NaCl
concentration up to 100 mM in culture medium led to reduce the growth
indicators (survival plants, average of the plant length and average number of
buds) with significant differences when compared with the control plants after
4 weeks from culturing on multiplication media, while treatment with 150 mM
of sodium chloride led to death of all plants. Using the concentrations 50 and
100 mM of NaCl also caused decreasing the total chlorophyll content in the
leaves.
This current study was carried out on B41 grape rootstock
micropropagated in vitro at the General Commission for Scientific Agricultural
Research (GCSAR), Syria to study the effect of different concentrations of
sodium chloride on the multiplicati
on and rooting stages. The results showed
that the highest average of survival plants (98%), the number of new buds
(8.43) and plant length (8.58 cm) were obtained in the control treatment after 4
weeks from multiplication stage. The treatment with 50 mM of sodium chloride
led to reduce the multiplication rates with significant differences, while the
lowest plant multiplication had occurred with 100 mM of sodium chloride and
no survival plants were remained by the treatment with 150 mM of sodium
chloride during multiplication stage. The highest rooting rates (% 85.30), the
number of roots (4.67) and root length (6.28 cm) were also obtained in the
control treatment after 4 weeks from rooting stage. The treatment with 50 mM
of sodium chloride led to reduce the rooting process with significant
differences. The lowest rooting results were observed with 100 mM of sodium
chloride and no rooting rate was observed when plants were treated with 150
mM of sodium chloride.
This current study was conducted on gardenia plant (Gardenia
jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect
of some plant growth regulators on multiplication and rooting, for the aim of
developing a complete in v
itro microprpagation system of the studied cv.
Results demonstrated that, the best medium for in vitro micropropagation of
the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA
with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73
cm, and average leaf number of 4.36. These shoots were then transferred to a
rooting media containing a half-strength of the multiplication basal media with
NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of
97% with average root number of 9.67, and average root length of 5.35 cm, and
average stem length of 9.80 cm, and average leaf number of 5.07. Increasing
NAA concentration in rooting media to 1.5 mg/L resulted in increasing root
length average to 6.28 cm with significant difference in comparison to the
previous treatment. Rooted Plantlets were acclimatized gradually in a growth
room conditions to ex vitro conditions with 85 % efficiency.
Potato (Solanum tuberosum L.) is among the most
economically important crops world-wide and in Syria
as well. It is highly responsive to different tissue culture
techniques.
In vitro culture of potato cv. 'Draga' (mid-early) was
established thro
ugh meristem tip culture.
Tissue culture process of date palm propagation via bud proliferation is
fully described. Adventitious plantlets were obtained from shoot tips. axillary
buds and the bottom of young leaves of five date palm cultivars (Sefri, Zahdi,
Rizzez, Khalas
and Hillaly).
