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The e effect of different concentrations of chloride sodium in multiplication and rooting in vitro micro- propagated of B41 grape rootstock

تأثير تراكيز مختلفة من كلوريد الصوديوم في إكثار أصل العنب B41 و تجذيره مخبريا (vitro In)

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 Publication date 2013
and research's language is العربية
 Created by Shamra Editor




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This current study was carried out on B41 grape rootstock micropropagated in vitro at the General Commission for Scientific Agricultural Research (GCSAR), Syria to study the effect of different concentrations of sodium chloride on the multiplication and rooting stages. The results showed that the highest average of survival plants (98%), the number of new buds (8.43) and plant length (8.58 cm) were obtained in the control treatment after 4 weeks from multiplication stage. The treatment with 50 mM of sodium chloride led to reduce the multiplication rates with significant differences, while the lowest plant multiplication had occurred with 100 mM of sodium chloride and no survival plants were remained by the treatment with 150 mM of sodium chloride during multiplication stage. The highest rooting rates (% 85.30), the number of roots (4.67) and root length (6.28 cm) were also obtained in the control treatment after 4 weeks from rooting stage. The treatment with 50 mM of sodium chloride led to reduce the rooting process with significant differences. The lowest rooting results were observed with 100 mM of sodium chloride and no rooting rate was observed when plants were treated with 150 mM of sodium chloride.


Artificial intelligence review:
Research summary
أجريت هذه الدراسة على أصل العنب B41 الذي تم إكثاره مخبرياً في الهيئة العامة للبحوث العلمية الزراعية بدمشق، لدراسة تأثير تراكيز مختلفة من كلوريد الصوديوم على مرحلتي الإكثار والتجذير. أظهرت النتائج أن أعلى نسبة نباتات متبقية (98%) وأكبر متوسط عدد للبراعم (8.43) وأكبر متوسط طول للنبات (8.58 سم) تم الحصول عليها في معاملة الشاهد بعد أربعة أسابيع من مرحلة الإكثار. بينما أدت المعاملة بتركيز 50 mM من كلوريد الصوديوم إلى خفض نتائج الإكثار بفروق معنوية عن معاملة الشاهد، وتم الحصول على أدنى النتائج بالمعاملة بتركيز 100 mM من كلوريد الصوديوم، في حين لم يتم الحصول على أي نسبة نباتات متبقية في المعاملة بتركيز 150 mM من كلوريد الصوديوم. في مرحلة التجذير، تم الحصول على أعلى نسبة تجذير (85.30%) وأعلى متوسط عدد جذور (4.67) وأكبر متوسط طول للجذور (6.28 سم) في معاملة الشاهد بعد أربعة أسابيع. بينما أدت المعاملة بتركيز 50 mM من كلوريد الصوديوم إلى خفض نتائج التجذير بفروق معنوية عن معاملة الشاهد، وتم الحصول على أدنى نتائج التجذير بالمعاملة بتركيز 100 mM من كلوريد الصوديوم، في حين لم يتم الحصول على أي نسبة تجذير في المعاملة بتركيز 150 mM من كلوريد الصوديوم.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة لفهم تأثير الملوحة على إكثار وتجذير أصل العنب B41، إلا أن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن استخدام مجموعة أوسع من التراكيز لتحديد النقطة المثلى بدقة أكبر. ثانياً، لم تتناول الدراسة تأثير الملوحة على الجوانب الأخرى للنبات مثل الإنتاجية والجودة. ثالثاً، كان من الممكن إجراء تجارب ميدانية للتحقق من النتائج المخبرية. وأخيراً، لم يتم مناقشة الآليات الفسيولوجية التي تؤدي إلى التأثيرات المرصودة بشكل كافٍ، مما يحد من فهمنا الكامل للتأثيرات الملاحية على هذا الأصل.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي هو تحديد درجة تحمل أصل العنب B41 للملوحة من خلال دراسة تأثير تراكيز مختلفة من كلوريد الصوديوم على مرحلتي الإكثار والتجذير المخبري.

  2. ما هي أعلى نسبة نباتات متبقية تم الحصول عليها في الدراسة؟

    أعلى نسبة نباتات متبقية تم الحصول عليها كانت 98% في معاملة الشاهد بعد أربعة أسابيع من مرحلة الإكثار.

  3. ما هو تأثير تركيز 150 mM من كلوريد الصوديوم على نسبة التجذير؟

    تركيز 150 mM من كلوريد الصوديوم أدى إلى عدم الحصول على أي نسبة تجذير.

  4. ما هي التراكيز المختلفة من كلوريد الصوديوم التي تم اختبارها في الدراسة؟

    التراكيز المختلفة من كلوريد الصوديوم التي تم اختبارها هي 0 (شاهد)، 50 mM، 100 mM، و150 mM.


References used
Alam, S. M., M. A. Khan. S. M. Mujtaba and A. Shereen, 2004. Influence of aqueous leaf extract of common lambsquarters and NaCl salinity on the germination, growth, and nutrient content of wheat, Acta Physiologiae Plantarum. 24 (4): 359-364
Barlass, M and K. G. M. Skene. 1981. Relative NaCl tolerances of grapevine cultivars and hybrids in vitro, Z Pflanzenphysiol. 102: 147-161
Bavaresco, L., M. Fregoni and E. Gambi. 1993. In vitro method to screen grapevine genotypes for tolerance to lime-induced chlorosis, Vitis. 32:145- 148
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