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Register a new userThe e effect of different concentrations of chloride sodium in multiplication and rooting in vitro micro- propagated of B41 grape rootstock
تأثير تراكيز مختلفة من كلوريد الصوديوم في إكثار أصل العنب B41 و تجذيره مخبريا (vitro In)
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أجري البحث على أصل العنب B41 الذي تم إكثاره مخبرياً (vitro in) في الهيئة العامة للبحـوث العلمية الزراعية بدمشق لدراسة تأثير تراكيز مختلفة من كلوريد الصوديوم في مرحلة الإكثار و التجـذير. أوضحت النتائج أن أعلى نسبة نباتات متبقية (%98) و أكبر متوسط عدد للبراعم (43.8) و أكبر متوسـط طول للنبات (58.8 سم) تم الحصول عليها في معاملة الشاهد بعد أربعة أسابيع من مرحلة الإكثـار، فـي حين أدت المعاملة بـ mM 50 من كلوريد الصوديوم إلى خفض نتائج الإكثار و بفـروق معنويـة عـن معاملة الشاهد، و قد تم الحصول على أدنى النتائج بالمعاملة mM 100 من كلوريـد الـصوديوم، بينمـا المعاملة بـ mM 150 من كلوريد الصوديوم لم يتم الحصول فيها على أي نسبة نباتات متبقية بمرحلـة الإكثار. كما حصلً على أعلى نسبة تجذير (30.85%) و أعلى متوسط عدد جذور (67.4) و أكبر متوسط طول للجذور (cm 28.6) في معاملة الشاهد بعد أربعة أسابيع من مرحلة التجذير، في حين أدت المعاملة بـ mM 50 من كلوريد الصوديوم إلى خفض نتائج التجذير و بفروق معنوية عن معاملة الشاهد، و قد تـم الحصول على أدنى نتائج التجذير بالمعاملة mM 100 من كلوريد الصوديوم، في حين أدت المعاملة بــ mM 150 من كلوريد الصوديوم إلى عدم الحصول على أي نسبة تجذير.
This current study was carried out on B41 grape rootstock micropropagated in vitro at the General Commission for Scientific Agricultural Research (GCSAR), Syria to study the effect of different concentrations of sodium chloride on the multiplication and rooting stages. The results showed that the highest average of survival plants (98%), the number of new buds (8.43) and plant length (8.58 cm) were obtained in the control treatment after 4 weeks from multiplication stage. The treatment with 50 mM of sodium chloride led to reduce the multiplication rates with significant differences, while the lowest plant multiplication had occurred with 100 mM of sodium chloride and no survival plants were remained by the treatment with 150 mM of sodium chloride during multiplication stage. The highest rooting rates (% 85.30), the number of roots (4.67) and root length (6.28 cm) were also obtained in the control treatment after 4 weeks from rooting stage. The treatment with 50 mM of sodium chloride led to reduce the rooting process with significant differences. The lowest rooting results were observed with 100 mM of sodium chloride and no rooting rate was observed when plants were treated with 150 mM of sodium chloride.
References used
Alam, S. M., M. A. Khan. S. M. Mujtaba and A. Shereen, 2004. Influence of aqueous leaf extract of common lambsquarters and NaCl salinity on the germination, growth, and nutrient content of wheat, Acta Physiologiae Plantarum. 24 (4): 359-364
Barlass, M and K. G. M. Skene. 1981. Relative NaCl tolerances of grapevine cultivars and hybrids in vitro, Z Pflanzenphysiol. 102: 147-161
Bavaresco, L., M. Fregoni and E. Gambi. 1993. In vitro method to screen grapevine genotypes for tolerance to lime-induced chlorosis, Vitis. 32:145- 148
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This current study was conducted on gardenia plant (Gardenia
jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect
of some plant growth regulators on multiplication and rooting, for the aim of
developing a complete in v
itro microprpagation system of the studied cv.
Results demonstrated that, the best medium for in vitro micropropagation of
the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA
with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73
cm, and average leaf number of 4.36. These shoots were then transferred to a
rooting media containing a half-strength of the multiplication basal media with
NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of
97% with average root number of 9.67, and average root length of 5.35 cm, and
average stem length of 9.80 cm, and average leaf number of 5.07. Increasing
NAA concentration in rooting media to 1.5 mg/L resulted in increasing root
length average to 6.28 cm with significant difference in comparison to the
previous treatment. Rooted Plantlets were acclimatized gradually in a growth
room conditions to ex vitro conditions with 85 % efficiency.
In this study, the effect of different concentrations of sodium chloride
(NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some
growth indicators of SO4 grape rootstock propagated in vitro was studied at the
laboratories of Gen
eral Commission for Scientific Agricultural Research
(GCSAR), Damascus/Syria. Results indicated that the increase of NaCl
concentration up to 100 mM in culture medium led to reduce the growth
indicators (survival plants, average of the plant length and average number of
buds) with significant differences when compared with the control plants after
4 weeks from culturing on multiplication media, while treatment with 150 mM
of sodium chloride led to death of all plants. Using the concentrations 50 and
100 mM of NaCl also caused decreasing the total chlorophyll content in the
leaves.
The trial was carried out during the season 2014, on grapevines
of Al-Baladi. Al Baladi, 20 years old, grafted onto rootstock, and trained in the trellis way, to study the influence of different levels of 4 times/season soil applied seaweed extract , on some qualitative and quantitative characteristics of Al-Baladi grapes.
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
This research was conducted at the Agriculture Faculty, Damascus
university to determine some factors affecting rooting of some grape rootstocks
(B41, Ru140, SO4) by hardwood cutting during two subsequent seasons. The
results showed that Rooting p
ercentage ranged between 50 to 65%. with
significant differences between tested rootstocks. The highest rooting
percentage was obtained on B41 rootstock reached 65.47% followed by SO4
and then by Ru140 which gave the lowest percentage 50.44%. The treatment
with hormone IBA increased rooting proportion and all the parameters
(number and length of root, number of leaves and shoot length) were
significantly higher than parallel parameters of the control. The storage of
cuttings at 4°C for 50 days increased significantly proportion of rooting and
number of roots compared with the storage under field condition. There were
significant differences among dates of planting where the third date (mid
February) showed superiority in all parameters studied over other dates. The
cuttings from basal and middle of annual growth gave a significant superiority
in the average root length (5.26–4.32 cm, successively) compared to the
terminal cuttings while there was no significant effect to the cuttings position on
rooting proportion.
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