Do you want to publish a course? Click here

The e effect of different concentrations of chloride sodium in multiplication and rooting in vitro micro- propagated of B41 grape rootstock

تأثير تراكيز مختلفة من كلوريد الصوديوم في إكثار أصل العنب B41 و تجذيره مخبريا (vitro In)

1432   0   35   0 ( 0 )
 Publication date 2013
and research's language is العربية
 Created by Shamra Editor




Ask ChatGPT about the research

This current study was carried out on B41 grape rootstock micropropagated in vitro at the General Commission for Scientific Agricultural Research (GCSAR), Syria to study the effect of different concentrations of sodium chloride on the multiplication and rooting stages. The results showed that the highest average of survival plants (98%), the number of new buds (8.43) and plant length (8.58 cm) were obtained in the control treatment after 4 weeks from multiplication stage. The treatment with 50 mM of sodium chloride led to reduce the multiplication rates with significant differences, while the lowest plant multiplication had occurred with 100 mM of sodium chloride and no survival plants were remained by the treatment with 150 mM of sodium chloride during multiplication stage. The highest rooting rates (% 85.30), the number of roots (4.67) and root length (6.28 cm) were also obtained in the control treatment after 4 weeks from rooting stage. The treatment with 50 mM of sodium chloride led to reduce the rooting process with significant differences. The lowest rooting results were observed with 100 mM of sodium chloride and no rooting rate was observed when plants were treated with 150 mM of sodium chloride.


Artificial intelligence review:
Research summary
أجريت هذه الدراسة على أصل العنب B41 الذي تم إكثاره مخبرياً في الهيئة العامة للبحوث العلمية الزراعية بدمشق، لدراسة تأثير تراكيز مختلفة من كلوريد الصوديوم على مرحلتي الإكثار والتجذير. أظهرت النتائج أن أعلى نسبة نباتات متبقية (98%) وأكبر متوسط عدد للبراعم (8.43) وأكبر متوسط طول للنبات (8.58 سم) تم الحصول عليها في معاملة الشاهد بعد أربعة أسابيع من مرحلة الإكثار. بينما أدت المعاملة بتركيز 50 mM من كلوريد الصوديوم إلى خفض نتائج الإكثار بفروق معنوية عن معاملة الشاهد، وتم الحصول على أدنى النتائج بالمعاملة بتركيز 100 mM من كلوريد الصوديوم، في حين لم يتم الحصول على أي نسبة نباتات متبقية في المعاملة بتركيز 150 mM من كلوريد الصوديوم. في مرحلة التجذير، تم الحصول على أعلى نسبة تجذير (85.30%) وأعلى متوسط عدد جذور (4.67) وأكبر متوسط طول للجذور (6.28 سم) في معاملة الشاهد بعد أربعة أسابيع. بينما أدت المعاملة بتركيز 50 mM من كلوريد الصوديوم إلى خفض نتائج التجذير بفروق معنوية عن معاملة الشاهد، وتم الحصول على أدنى نتائج التجذير بالمعاملة بتركيز 100 mM من كلوريد الصوديوم، في حين لم يتم الحصول على أي نسبة تجذير في المعاملة بتركيز 150 mM من كلوريد الصوديوم.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة لفهم تأثير الملوحة على إكثار وتجذير أصل العنب B41، إلا أن هناك بعض النقاط التي يمكن تحسينها. أولاً، كان من الممكن استخدام مجموعة أوسع من التراكيز لتحديد النقطة المثلى بدقة أكبر. ثانياً، لم تتناول الدراسة تأثير الملوحة على الجوانب الأخرى للنبات مثل الإنتاجية والجودة. ثالثاً، كان من الممكن إجراء تجارب ميدانية للتحقق من النتائج المخبرية. وأخيراً، لم يتم مناقشة الآليات الفسيولوجية التي تؤدي إلى التأثيرات المرصودة بشكل كافٍ، مما يحد من فهمنا الكامل للتأثيرات الملاحية على هذا الأصل.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي هو تحديد درجة تحمل أصل العنب B41 للملوحة من خلال دراسة تأثير تراكيز مختلفة من كلوريد الصوديوم على مرحلتي الإكثار والتجذير المخبري.

  2. ما هي أعلى نسبة نباتات متبقية تم الحصول عليها في الدراسة؟

    أعلى نسبة نباتات متبقية تم الحصول عليها كانت 98% في معاملة الشاهد بعد أربعة أسابيع من مرحلة الإكثار.

  3. ما هو تأثير تركيز 150 mM من كلوريد الصوديوم على نسبة التجذير؟

    تركيز 150 mM من كلوريد الصوديوم أدى إلى عدم الحصول على أي نسبة تجذير.

  4. ما هي التراكيز المختلفة من كلوريد الصوديوم التي تم اختبارها في الدراسة؟

    التراكيز المختلفة من كلوريد الصوديوم التي تم اختبارها هي 0 (شاهد)، 50 mM، 100 mM، و150 mM.


References used
Alam, S. M., M. A. Khan. S. M. Mujtaba and A. Shereen, 2004. Influence of aqueous leaf extract of common lambsquarters and NaCl salinity on the germination, growth, and nutrient content of wheat, Acta Physiologiae Plantarum. 24 (4): 359-364
Barlass, M and K. G. M. Skene. 1981. Relative NaCl tolerances of grapevine cultivars and hybrids in vitro, Z Pflanzenphysiol. 102: 147-161
Bavaresco, L., M. Fregoni and E. Gambi. 1993. In vitro method to screen grapevine genotypes for tolerance to lime-induced chlorosis, Vitis. 32:145- 148
rate research

Read More

This current study was conducted on gardenia plant (Gardenia jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect of some plant growth regulators on multiplication and rooting, for the aim of developing a complete in v itro microprpagation system of the studied cv. Results demonstrated that, the best medium for in vitro micropropagation of the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73 cm, and average leaf number of 4.36. These shoots were then transferred to a rooting media containing a half-strength of the multiplication basal media with NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of 97% with average root number of 9.67, and average root length of 5.35 cm, and average stem length of 9.80 cm, and average leaf number of 5.07. Increasing NAA concentration in rooting media to 1.5 mg/L resulted in increasing root length average to 6.28 cm with significant difference in comparison to the previous treatment. Rooted Plantlets were acclimatized gradually in a growth room conditions to ex vitro conditions with 85 % efficiency.
In this study, the effect of different concentrations of sodium chloride (NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some growth indicators of SO4 grape rootstock propagated in vitro was studied at the laboratories of Gen eral Commission for Scientific Agricultural Research (GCSAR), Damascus/Syria. Results indicated that the increase of NaCl concentration up to 100 mM in culture medium led to reduce the growth indicators (survival plants, average of the plant length and average number of buds) with significant differences when compared with the control plants after 4 weeks from culturing on multiplication media, while treatment with 150 mM of sodium chloride led to death of all plants. Using the concentrations 50 and 100 mM of NaCl also caused decreasing the total chlorophyll content in the leaves.
The trial was carried out during the season 2014, on grapevines of Al-Baladi. Al Baladi, 20 years old, grafted onto rootstock, and trained in the trellis way, to study the influence of different levels of 4 times/season soil applied seaweed extract , on some qualitative and quantitative characteristics of Al-Baladi grapes.
This investigation was conducted on Ru140 grape rootstock at the General Commission for Scientific Agricultural Research (GCSAR), Damascus with the aim of in vitro vegetatively micropropagation using some plant growth regulators on multiplication and rooting to determine the best combinations and concentrations of plant growth regulators that result in the best multiplication rate, and best rooting crekeria (rate and roots number and length). Results demonstrated that, the best medium for in vitro micropropagation of the studied rootstock was the modified MS medium supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72 new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were transferred for 4 weeks to among elongation medium containing the same medium with the addition of Kinetine at a concentration of 2.22 μM instead of BA which led to a shoot elongation rate of 7.87 cm, then these shoots were transferred to rooting medium for rooting, It was shown that using auxin IBA at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with the largest number of roots (7.56) when using the auxin IBA concentration μM 4.44 compared with the rest of other transactions and with the control as well. However, The highest length of roots (6.29 cm) was observed on medim contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized gradually to ex vitro conditions with 70 % efficiency.
This research was conducted at the Agriculture Faculty, Damascus university to determine some factors affecting rooting of some grape rootstocks (B41, Ru140, SO4) by hardwood cutting during two subsequent seasons. The results showed that Rooting p ercentage ranged between 50 to 65%. with significant differences between tested rootstocks. The highest rooting percentage was obtained on B41 rootstock reached 65.47% followed by SO4 and then by Ru140 which gave the lowest percentage 50.44%. The treatment with hormone IBA increased rooting proportion and all the parameters (number and length of root, number of leaves and shoot length) were significantly higher than parallel parameters of the control. The storage of cuttings at 4°C for 50 days increased significantly proportion of rooting and number of roots compared with the storage under field condition. There were significant differences among dates of planting where the third date (mid February) showed superiority in all parameters studied over other dates. The cuttings from basal and middle of annual growth gave a significant superiority in the average root length (5.26–4.32 cm, successively) compared to the terminal cuttings while there was no significant effect to the cuttings position on rooting proportion.
comments
Fetching comments Fetching comments
Sign in to be able to follow your search criteria
mircosoft-partner

هل ترغب بارسال اشعارات عن اخر التحديثات في شمرا-اكاديميا