A study of Some Factors Affecting the Vegetative Micropropagation of Ornamental Viburnum Bush (Viburnum Tinus L.)

This study was conducted during 2012-2013 in order to efficiently micropropagate viburnum (Viburnum tinus) bushes using tissue culture techniques. The shoots were cultured on a Murashige and Skoog medium supplemented with 30 gl-1 sucrose, 7 gl-1 agar - agar, citric acid as an anti-oxidant (300 mgl-1), and different concentrations of benzyl amino purine and Naphthalene Acetic Acid. A media of Murashige and Skoog was used for laboratory rooting after reducing the major mineral elements to the half, reducing the sucrose to 20 gl-1, and addingindole-3-butyric acid (IBA) of different concentrations (0, 0.5, 1, 1.5 mgl-1). The results showed that it is necessary to have auxin and cytokinin in culture to improve the value of open buds and the number of shoots per initial explants. The concentrations 0.25 mgl-1 from NAA with 1 mgl-1 from BAP gave the highest value of open buds (93.33%) and the maximum number of shoot per initial explants (1.57). To improve the number and length of the shoots produced, the solution mineral (MS) was replaced with another: media woody plants (WPM) which gave better elongation for the resulting growths (3.21 cm) and a better number of shoots (2.72 Growth/explant) compared to the media (MS) using the same compatibility hormone (0.25 mgl-1 of NAA and 1 mgl-1 of BAP). The results also show that the highest percentage of rooting reached (84.44%) with (0.5 mgl-1) IBA which was better than (1.5 mgl-1) IBA and better than the treatment of the control. Results also showed that the best medium for the length and number of roots formed was (2.7cm, 3.82root) when the concentration was (0.5 mgl-1) surpassing the control. The success rate of the acclimatization of the resulting laboratory plantlets under glasshouse conditions reached (73.33%) one month and a half after the transplanting.

Effect of some growth regulators on in vitro micropropagation of Ru140 grape rootstock

This investigation was conducted on Ru140 grape rootstock at the General Commission for Scientific Agricultural Research (GCSAR), Damascus with the aim of in vitro vegetatively micropropagation using some plant growth regulators on multiplication and rooting to determine the best combinations and concentrations of plant growth regulators that result in the best multiplication rate, and best rooting crekeria (rate and roots number and length). Results demonstrated that, the best medium for in vitro micropropagation of the studied rootstock was the modified MS medium supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72 new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were transferred for 4 weeks to among elongation medium containing the same medium with the addition of Kinetine at a concentration of 2.22 μM instead of BA which led to a shoot elongation rate of 7.87 cm, then these shoots were transferred to rooting medium for rooting, It was shown that using auxin IBA at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with the largest number of roots (7.56) when using the auxin IBA concentration μM 4.44 compared with the rest of other transactions and with the control as well. However, The highest length of roots (6.29 cm) was observed on medim contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized gradually to ex vitro conditions with 70 % efficiency.

Effects of some factors influencing on in vitro tuberization of potato cv. «Draga»

Potato (Solanum tuberosum L.) is among the most economically important crops world-wide and in Syria as well. It is highly responsive to different tissue culture techniques. In vitro culture of potato cv. 'Draga' (mid-early) was established through meristem tip culture.

In Vitro Multiplication of Bitter Almond (Amygdalus communis L.)

In vitro rooting was significantly increased by adding indolebutyric acid (IBA) to medium and rooting was improved by adding ١,٠ g/L activated charcoal or ٠,١ g/L polyvinylpyrrolidone (PVP). Indoleacetic acid (IAA) (٠,٠ to ٤,٠ mg/L) was not effective for rooting except when used with ٠,١ g/L PVP. A ٩٥٪ survival was achieved when plants were acclimatized ex vitro. Such procedures could help significantly in clonally propagating bitter almond and conserving its germplasm.

In Vitro Propagation and In Vivo Acclimatization of Sour Orange (Citrus aurantium L.)

Some factors which affect in vitro propagation of sour orange (Citrus aurantium. L.) were studied on Murashige and Skoog medium. Medium containing ١,٠ mg/L ٦-Benzylaminopurine (BA) was satisfactory for shoot multiplication, Dichlorophenoxyacetic acid (٢،٤-D) was not effective for shoot proliferation and it enhanced callus formation.