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Register a new userThe effect of sucrose on the chlorophyll content of healthy and vitrificated plants of some species of Gypsophylla panicolata micropropagated in vitro
تأثير السكروز في محتوى اليخضور للنباتات السليمة و المصابة بالشفافية لبعض أصناف نبات الجبسوفيلا المكاثر مخبرياً
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درس تأثير استخدام ثلاثة تراكيز (10،30،50 غ/ل) من السكروز فـي محتـوى اليخـضور لثلاثـة أصناف من الجبسوفيلا (Golantwo ،Planshnet ،وNewlove) و أجريت المقارنـة بـين النباتـات السليمة و النباتات الشفافة. بينت النتـائج تفـوق المعاملـة 10 غ/ل سـكروز فـي تخفـيض الـشفافية (p > 01.0) إلى 78.36 % بالمقارنة مع معاملة الشاهد (30 غ/ل) البالغة 57.62 % كمـا وجـد فـرق معنوي بين الأصناف (p > 01.0) ؛ حيث تفوق الصنف Planshnet بمحتواه من اليخضور في كل مـن النباتات الشفافة و النباتات السليمة، و رافق ذلك تفوق المعاملة (10)غ/ل بزيادة محتوى اليخضور مقارنة مع باقي المعاملات، إذ بلغ محتوى اليخضور الكلي عند النباتات السليمه 46.17 ميكروغرام/مل بالمقارنه مع النباتات الشفافه (26.14 ميكروغرام/مل)، في الصنف Planshnet.
This study was conducted at the laboratories of Horticultural Department, Faculty of Agriculture, Damascus University to determine the effect of using three levels of sucrose (10, 30, 50 g /l) on chlorophyll content between healthy and vitrified plants of three varieties of Gypsophylla panicolata (Golantwo, Planshnet, Newlove). Results showed superiority of treatment 10 g / l sucrose in reducing vitrification percent to 36.78% compared with 62.57% for control (30 g/l) and with significant differences between varieties. Planshnet was the best variety in chlorophyll content either in healthy or in vitrified plantlets. Moreover, the treatment with sucrose of 10 g/l caused significant increment of chlorophyll content compared with other treatments. The high total chlorophyll content was 17.46 micrograms/ ml in healthy plants compared with 14.26 micrograms / ml in vitrification plants for Planshnet at 10 g/l sucrose.
Artificial intelligence review:
Research summary
تناولت الدراسة تأثير استخدام تراكيز مختلفة من السكروز (10، 30، 50 غ/ل) على محتوى اليخضور في نباتات الجبسوفيلا المكاثرة مخبرياً، سواء كانت سليمة أو مصابة بالشفافية. أظهرت النتائج أن التركيز المنخفض من السكروز (10 غ/ل) كان الأكثر فعالية في تقليل نسبة الشفافية إلى 36.78% مقارنة بالشاهد (30 غ/ل) الذي بلغت نسبة الشفافية فيه 62.57%. كما أظهرت الدراسة أن الصنف Planshnet تفوق في محتواه من اليخضور في كل من النباتات السليمة والمصابة بالشفافية، حيث بلغ محتوى اليخضور الكلي في النباتات السليمة 17.46 ميكروغرام/مل مقارنة بالنباتات المصابة بالشفافية (14.26 ميكروغرام/مل) عند استخدام تركيز 10 غ/ل من السكروز. وأظهرت الدراسة أيضاً وجود علاقة ارتباط عكسية قوية بين تركيز السكروز ومحتوى اليخضور، حيث انخفض محتوى اليخضور مع زيادة تركيز السكروز في الوسط.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة في مجال زراعة الأنسجة النباتية، حيث تسلط الضوء على تأثير تراكيز السكروز المختلفة على محتوى اليخضور ونسبة الشفافية في نباتات الجبسوفيلا. ومع ذلك، يمكن توجيه بعض النقد البناء للدراسة. أولاً، كان من الممكن توسيع نطاق الدراسة لتشمل أنواع نباتية أخرى لزيادة تعميم النتائج. ثانياً، لم تتناول الدراسة بشكل كافٍ تأثير العوامل البيئية الأخرى مثل درجة الحرارة والرطوبة والإضاءة على نسبة الشفافية ومحتوى اليخضور. ثالثاً، كان من الممكن استخدام تقنيات تحليلية أكثر تقدماً لقياس محتوى اليخضور بدقة أكبر. وأخيراً، كان من الممكن تقديم توصيات عملية أكثر وضوحاً للمزارعين بناءً على النتائج.
Questions related to the research
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ما هو الهدف الرئيسي من الدراسة؟
الهدف الرئيسي من الدراسة هو تحديد تأثير تراكيز مختلفة من السكروز على محتوى اليخضور ونسبة الشفافية في نباتات الجبسوفيلا المكاثرة مخبرياً.
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ما هي التراكيز المستخدمة من السكروز في الدراسة؟
استخدمت الدراسة ثلاثة تراكيز من السكروز: 10 غ/ل، 30 غ/ل، و50 غ/ل.
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ما هو الصنف الذي تفوق في محتواه من اليخضور؟
الصنف Planshnet تفوق في محتواه من اليخضور في كل من النباتات السليمة والمصابة بالشفافية.
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ما هي العلاقة بين تركيز السكروز ومحتوى اليخضور؟
أظهرت الدراسة وجود علاقة ارتباط عكسية قوية بين تركيز السكروز ومحتوى اليخضور، حيث انخفض محتوى اليخضور مع زيادة تركيز السكروز في الوسط.
References used
Ault, J. R. 1992. In vitro propagation of a Silene hybrid (S.polyPlanshnettala * virginica). Hort Sience, 27:1226
Capellades, M., R. Lemeur and P. C. Debergh. 1991. Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro. Plant Cell. Tiss and Org Cult, 25:19-20
Demeester, J. J., D. G. Matthÿs., B. Pascat and P. C. Debergh. 1995. Toward a controllable headspace composition. Growth, development, and headspace of a micropropagated Prunus rootstock in different containers. In Vitro Cell. Dev. Biol, 31:105-112
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