استخدمت في هذه التجربة عقل ساقية نصف قاسية في الموعدين الخريفي و الربيعي (أيلول و آذار) و التي عوملت بأربعة تراكيز من الأوكسين IBA) 2000, 4000, 6000,8000) جزء بالمليون بطريقة الغمس السريع إضافة إلى الشاهد، ثم زرعت بشكل عشوائي على أوساط تجذير مختلفة.
أشارت النتائج إلى تفوق الوسط (فيرميكولايت+ بتموس) معنوياً على كافة الأوساط المستخدمة عدا وسط البرليت في نسبة التجذير (90.5%)، و في متوسط طول الجذور عدا وسط البتموس منفرداً (10.9 سم)، و في عدد الجذور المتشكلة لكن على جميع الأوساط المدروسة (7.19 جذر/عقلة)، و ذلك باستخدام التراكيز 6000 و 4000 و 8000 جزء بالمليون على التوالي.
تفوق موعد أخذ العقل في الخريف معنوياً على موعد أخذ العقل في الربيع في نسبة التجذير عند استخدام التركيز 6000 جزء بالمليون، إذ وصلت إلى أقصاها (100%)، و في متوسط عدد الجذور الذي بلغ ( 10.30 جذر/عقلة) عند استخدام التركيز 8000 جزء بالمليون. و أيضاً في متوسط طول الجذور عند التركيز 4000 جزء بالمليون (11.69سم)، حيث يمكن ربط ذلك بالحالة الفسيولوجية للعقل التي تختلف حسب الفصول.
كان للتراكيز الهرمونية تأثيرٌ واضحٌ في نسبة تفتح البراعم و التجذير و نسبة تشكل الكالس على قواعد العقل المعاملة، حيث ازدادت قيمها بازدياد التركيز الهرموني عدا نسبة التجذير التي انخفضت نسبياً عند8000 جزء بالمليون.
أظهرت العقل المجذرة قدرة عالية على التأقلم مع ظروف البيت الزجاجي ثم مع ظروف الوسط الخارجي، حيث بلغت نسبة نجاح التقسية 90% بعد شهرين من نقلها عند النوع المدروس.
This study was conducted during 2012-2013 in order to efficiently micropropagate
viburnum (Viburnum tinus) bushes using tissue culture techniques. The shoots were
cultured on a Murashige and Skoog medium supplemented with 30 gl-1 sucrose, 7 gl-1 ag
ar
- agar, citric acid as an anti-oxidant (300 mgl-1), and different concentrations of benzyl
amino purine and Naphthalene Acetic Acid. A media of Murashige and Skoog was used
for laboratory rooting after reducing the major mineral elements to the half, reducing the
sucrose to 20 gl-1, and addingindole-3-butyric acid (IBA) of different concentrations (0,
0.5, 1, 1.5 mgl-1). The results showed that it is necessary to have auxin and cytokinin in
culture to improve the value of open buds and the number of shoots per initial explants.
The concentrations 0.25 mgl-1 from NAA with 1 mgl-1 from BAP gave the highest value of
open buds (93.33%) and the maximum number of shoot per initial explants (1.57). To
improve the number and length of the shoots produced, the solution mineral (MS) was
replaced with another: media woody plants (WPM) which gave better elongation for the
resulting growths (3.21 cm) and a better number of shoots (2.72 Growth/explant)
compared to the media (MS) using the same compatibility hormone (0.25 mgl-1 of NAA
and 1 mgl-1 of BAP).
The results also show that the highest percentage of rooting reached (84.44%) with
(0.5 mgl-1) IBA which was better than (1.5 mgl-1) IBA and better than the treatment of the
control. Results also showed that the best medium for the length and number of roots
formed was (2.7cm, 3.82root) when the concentration was (0.5 mgl-1) surpassing the
control. The success rate of the acclimatization of the resulting laboratory plantlets under
glasshouse conditions reached (73.33%) one month and a half after the transplanting.
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
This study was conducted at glasshouse of Citrus and Tropical Research Department
in Tartus governorate, and Olive Nursery in Latakia governorate, during 2015 to
determine the influence of Indol-3-Butyric Acid (IBA) doses on hardwood and
softwood
top cuttings of three types of M. alba L. (B-1, B-5, KH-8) and three types
of M. nigra L. (M-4, KH-9, KH-1) and one fruitless type (B-3), which spreads
in different locations in Tartus, Syria. Hardwood cuttings were prepared during
February from one-year-old shoots, and the softwood top cuttings were prepared
during August in 2015. The cuttings were treated with different doses of IBA (1000,
2000 and 4000 ppm) in addition to the control application 0 ppm. The cuttings were
planted in the glasshouses in order to root.
