This research was conducated on Ceratonia siliqua L. in vitro to study the effect of
some growth regulators in both multiplication and rooting stage. And determine the best
concentrations wich lead the highest rate of multiplication (interms of number of shoots
and length), and the best rooting (interms of percentage of rooting, number of roots and
length).
This study was conducted at the laboratories of Horticultural Department, Faculty
of Agriculture, Damascus University to determine the effect of using three levels of
sucrose (10, 30, 50 g /l) on chlorophyll content between healthy and vitrified pl
ants of
three varieties of Gypsophylla panicolata (Golantwo, Planshnet, Newlove). Results
showed superiority of treatment 10 g / l sucrose in reducing vitrification percent to
36.78% compared with 62.57% for control (30 g/l) and with significant differences
between varieties. Planshnet was the best variety in chlorophyll content either in healthy
or in vitrified plantlets. Moreover, the treatment with sucrose of 10 g/l caused significant
increment of chlorophyll content compared with other treatments. The high total
chlorophyll content was 17.46 micrograms/ ml in healthy plants compared with 14.26
micrograms / ml in vitrification plants for Planshnet at 10 g/l sucrose.
In this study, we used plant tissue culture techniques for micropropagation
of the endangered Red Doumani cultivar. The explants which were the apex
tips and axillary buds (0.5-1 cm) have been cultured on the free of hormones
initial medium WPM (W
oody plant medium), then moved to the
micropropagation media. The results showed that the medium containing 4.44
mM BA + 0.58 mM IAA had the best effect on number of new shoots formed
(2.6) and the growth started after 9.22 days, the best elongation was on the
medium supplemented with 4.6 mM KIN + 0.58 mM IAA and the rate of
multiplication reached 5.15 shoots.
