A reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used
to detect infectious bronchitis virus (IBV) in the commercial broiler flocks in
Syria. 50 tissue samples were taken from tracheal tissue, trachea and kidney of
the broil
ers suspected of infectious bronchitis (IB) from different governorates
of Syria i.e. Latakia, Tartous, Hama and Damascus countryside. RNA was
extracted directly from the tissue samples and then RNA was converted to cDNA
by RT-PCR technology; PCR reaction and Nested PCR interaction were carried
out sequentially. The primers used in the RT-PCR reaction were selected from
the S1 gene (spike), where mutations of the virus genome were concentrated in
this region (the hypervariable region). Some positive samples (10) were injected
at an age of 9-11 days old SPFEE-specific pathogen free embryonated eggs
according to the methods adopted in virology. This research was carried out at
the laboratories of Latakia Research Center, General Commission for Scientific
Agricultural Research GCSAR, in cooperation with the PCR laboratory at the
Faculty of Veterinary Medicine in Hama. The results showed the existence of 37
positive case for RT-PCR (74%), and the infectious embryos showed clear and
characteristic anatomical lesions of the infectious bronchitis virus after 5-6 days
post injection, delayed and undeveloped fetal (dwarfism), fingertip entanglement
and hemorrhage compared with the negative control. The results also showed the
sensitivity and speed of the RT-PCR test in the detection of the IBV virus.
Chronic periodontitis (CP) is an inflammatory disease, characterized by gingivitis,
and affecting tooth supporting tissues, forming periodontal pockets with associated
attachment loss, alveolar bone resorption. It is common in adults, but can also
occur at any
age. The rate of disease progression may be influenced by local, systemic conditions,
and/or environmental factors that alter the normal host response to bacterial plaque, and
affect the susceptibility to disease.It is suggested that periodontitis is partially associated
by genetic factors, that many genes are involved in inflammation susceptibility, mainly
include the vitamin D receptor (VDR) gene that is implicated in bone metabolism and the
host immune response.
80 Syrian subjects were recruited for vitamin D receptor gene polymorphism study,
and allocated in two groups: 50 diagnosed with CP and mean age was (64 ± 0.722) years,
30 matched controls. DNA was isolated from peripheral blood cells, and genotyping was
performed by polymerase chain reaction (PCR) method and restriction fragment length
polymorphism analysis (RFLP) by using FokI enzyme.
By using Chi square test, no significant differences were found between the study
groups in the frequencies of alleles and genotypes at FokI position of VDR gene, age and
sex. These findings suggest that the investigated factors are not associated with periodontal
disease in this studied sample of Syrian population.
This study was carried out to detect the bacteria which caused Carp
Erythrodermatitis in the governorate of Hama, by using bacterial
isolation ,and polymerase chain reaction (PCR) , the bacterial species
were defined by biochemical Tests
This research was conducted at the Department of Food Science,
Faculty of Agriculture and National commission for posterity
energy. Twenty kg of apple juice concentrate (70%) were tooked
from company of Natural Aljabal Juice from AL-suidaa
Govern
orate and prarerd two concentarate (15% and 35%) by using
distilled –sterlization water according to pirson .
In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
The aim of this research was detection Listeria in chopped
prepared to consumption beef. (100) samples were collected
randomly from Damascus and its countryside, Kenitra and
Sweida stores and slaughterhouses, from February (2015) to
February (2016).
Several protocols for DNA extraction from leaves of pepper Capsicum annuum
L. were evaluated to detect Begomoviruses. The extraction
methods comparison were based on DNA quantity and quality. The
DNA concentration absorbance for the evaluation of DNA concentration and contamination
were measured using spectrophotometer.
تقييم طريقة Multiplex PCR لكشف نسخ الرنا المرسال للجين المندمج BCR-AbL لدى مرضى الابيضاض النقوي المزمن
This study were conducted on 450 samples of raw chicken meat
( 150 samples of thighs- 150 samples of breasts -150 samples of
wings ) were collected from retail market in Hama city, to detect
contamination of staphylococcus. Bacteriological and bio
chemical
tests results showed 407 samples positive for staphylococcus .
contained S. aureus 73 (16.2% ) isolated and coagulase-negative
Staphylococcus 334 (83.8%) isolated out of total samples. The
results of Multiplex PCR test conducted on S. aureus isolates,
showed that 37(50.7%) isolates harbored at least one enterotoxin
gene, with sea being the most frequently encountered ones.
أجريت الدراسة في مخابر كلية الزراعة قسم علوم الاغذية ومخابر الميكروبيولوجيا والمناعيات بقسم البيولوجيا الجزيئية والتقانة الحيوية بهيئة الطاقة الذرية.
