Spices are becoming increasingly important world-wide due to their effectiveness as natural antibiotics, the thing that makes the world nowadays tend to use spices instead of chemical drugs. However, this doesn’t mean they are free of some dangerous
pathogens such as Klebsiella spp. which can lead to a wide range of illnesses, notably pneumonia, urinary tract infections (UTIs), and bacteremia. Although we don’t know the natural habitat of this bacterium we find that it thrives in herbs and spices, concluding thus that plants may be its natural habitat.
The primary isolation of this bacterium was based first on its growth on general media and then isolation on selective media. Colonies of the microorganisms were counted on different media then Klebsiella was isolated from a selective media. The isolated colonies of Klebsiella were circular, dome-shaped, 3-4 mm in diameter, with mucoid sticky edges, and surrounded by capsule. After this we chose the typical colony and identified it using Polymerase chain reaction PCR and biochemical tests. Our results showed that 32% of examined specimen contained Klebsiella spp.
Economic losses resulting from the impact of brucellosis on animals have
increased, which is reflected in turn on humans. Brucella is pathogenic bacteria
shared by humans and pet animals. They still pose a significant risk that
threatens health an
d economics in Syria. Many efforts have focused to prevent
the spread of brucellosis disease, by resorting to the use of vaccines prepared
from Brucella strains, where now the target is preparation of vaccine
components proteins of Brucella can be used as a vaccine or an improved
traditional vaccine.
Thirteen viable and post - frozenthawed Shami cows embryos obtained by
super-ovulation and artificial insemination technique in order to determine sex
of embryos. Biopsies (1-2 cells) of embryos at morula or blastocyst stage were
taken using micro
manipulation under inverted microscope.
It was concluded that aspiration
of 1-2 blastomere (s) from embryos at morula or blastocyst stage was enough to
determine sex of embryo using PCR and it is recommended that using this
technique can be a useful tool in splitting and transferring of desired sex
embryos in genetic improvement program.
This study aimed to investigate the possibility of rapid identification of
isolated bacteria Leuconostoc from Syrian white fresh cheese made of cow and
ewe milk using Fourier transform infrared spectroscopy (FTIR). 20 isolates (14
from ewe milk ch
eese and 6 from cow milk cheese) were isolated. These isolates
were pretasted with PCR technique using specific primers. Results indicated
that these isolates are belonged to the genus Leuconostoc and 12 of them are
belonged to Ln. mesenteroides species. The results of FTIR technique showed in
comparison to device library that all isolates are belonged to genus
Leuconostoc, and 4 of these isolates are belonged to Ln. citreum species which
could not be identified using PCR method (with the used primers), and helped
to identify the following subspecies: Ln.mesenteroides subsp mesenteroides and
Ln. mesenteroides subsp dextranicum while PCR technique described them
belonging only to a Ln. mesenteroides species. Moreover, FTIR technique was
able to give the results within 25 hours, thus it can be used in identification of
food bacteria more quickly.
This study was conducted in the years 2008-2009-2010, it has analyzed for
Staph. aureus and isolated from 100 samples of traditional ice cream sold to
customers it has had collected from different regions parts of Damascus and his
countryside. The
study has been found that there was no violation of the Syrian
standard for Staph. aureus. And the results were also diagnosed using the
techniques of API Staph and PCR equal.
This research was conducted at the Department of Food Science,
Faculty of Agriculture and National commission for posterity
energy. Twenty kg of apple juice concentrate (70%) were tooked
from company of Natural Aljabal Juice from AL-suidaa
Govern
orate and prarerd two concentarate (15% and 35%) by using
distilled –sterlization water according to pirson .
The aim of this research was detection Listeria in chopped
prepared to consumption beef. (100) samples were collected
randomly from Damascus and its countryside, Kenitra and
Sweida stores and slaughterhouses, from February (2015) to
February (2016).
Chronic periodontitis (CP) is an inflammatory disease, characterized by gingivitis,
and affecting tooth supporting tissues, forming periodontal pockets with associated
attachment loss, alveolar bone resorption. It is common in adults, but can also
occur at any
age. The rate of disease progression may be influenced by local, systemic conditions,
and/or environmental factors that alter the normal host response to bacterial plaque, and
affect the susceptibility to disease.It is suggested that periodontitis is partially associated
by genetic factors, that many genes are involved in inflammation susceptibility, mainly
include the vitamin D receptor (VDR) gene that is implicated in bone metabolism and the
host immune response.
80 Syrian subjects were recruited for vitamin D receptor gene polymorphism study,
and allocated in two groups: 50 diagnosed with CP and mean age was (64 ± 0.722) years,
30 matched controls. DNA was isolated from peripheral blood cells, and genotyping was
performed by polymerase chain reaction (PCR) method and restriction fragment length
polymorphism analysis (RFLP) by using FokI enzyme.
By using Chi square test, no significant differences were found between the study
groups in the frequencies of alleles and genotypes at FokI position of VDR gene, age and
sex. These findings suggest that the investigated factors are not associated with periodontal
disease in this studied sample of Syrian population.
