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Register a new userStudying the Presence and Expression of P27 Gene in Promastigotes of Leishmania tropica
دراسة تواجد جين P27 في الليشمانية المدارية L.tropica و دراسة تعبيرها الجيني في مشيقات Promastigote الليشمانية المدارية
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Aِl-Baath University ورقة بحثية
Publication date
2017
fields
Pharmacy
and research's language is
العربية
Created by
Shamra Editor
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في هذه الدراسة قمنا بدراسة التعبير الجيني عن P27 في الشكل المشيقي لليشمانية المدارية بواسطة تقانة Reverse Transcriptase Polymerase Chain RT-PCR) Reaction) مستخدمين زوجاً من المشارع النوعية التي تم تصميمها يدوياً بالاعتماد على تسلسل هذه الجين في الأنواع الأخرى من الليشمانية بعد تحديد و معرفة المناطق المصانة من التسلسل، و أظهرت النتائج إثبات تواجد جين P27 في جينوم الليشمانية المدارية إلى جانب وجود تعبير جيني لها في الشكل المشيقي.
In our study, the presence and expression of P27 gene in L.tropica evaluated in promastigotes by PCR and RT-PCR using specific primer pairs that manually designed after determine the consensus sequence of forward and reverse region of primers between species of Leishmania by using MUSCLE (Multiple Sequence Comparison by Log- Expectation) tool for alignment. The results proved the presence of P27 gene in Leishmania tropica, beside presence the mRNA of P27 gene that confirm that P27 expressed in promastigote form of L.tropica
References used
Bari, A. U. and S. B. Rahman (2008). "Cutaneous leishmaniasis: an overview of parasitology and hostparasite- vector inter relationship." JPAD 18: 42-48
Desjeux, P. (2004). "Leishmaniasis: current situation and new perspectives." Comp Immunol Microbiol Infect Dis 27(5): 305-318
Dey, R., et al. (2010). "Characterization of a Leishmania stage-specific mitochondrial membrane protein that enhances the activity of cytochrome c oxidase and its role in virulence." Mol Microbiol 77(2): 399-414
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The leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania tropica parasite , the difficulty of
controlling the reservoirs of parasites, and there is no specific ,safe
and active therapy .Due to lack of studies
of Leishmania tropica
genome , as initial step we detect the presence of SW3 gene.
the leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania Tropica parasite , the hardness of
controlling the reservoirs of parasites, and there is no specific ,safe
active therapy. A Heat shock protein 20 HSP2
0 belongs to the
small HSP (sHSP) family.
Kinetoplastid membrane protein-11 (KMP-11), a protein present in all kinetoplastid
protozoa studied up to date, is considered a potential vaccine candidate for Leishmaniasis.
Such vaccine molecules must be expressed in amastigotes which represent t
he infective
forms for mammals, while promastigotes are the flagellate forms found in the insect hosts.
However, the expression of KMP-11 in amastigotes is still a subject of controversy. In this
study, a strain of L. tropica was isolated, cultivated, and genotyped. The expression of
KMP-11 gene in this strain was evaluated in promastigotes and in amastigotes by RT-PCR
using specific primer pairs. The results proved the presence of mRNA of KMP-11 in both
promastigotes and amastigotes forms of L. tropica. The expression of this molecule in
amastigotes is consistent with the previously demonstrated immunoprotective capacity of
KMP-11 DNA vaccine as well as the presence of humoral and cellular immune responses
against KMP-11 in Leishmania-infected animals.
This study evaluated the in vitro
antileishmanial activity of some Phenolic compounds including:
Ferulic acid, Mehtyl Ferulate, Ethyl Ferulate, and Sinapic acid
against leishmanial tropica promastigotes. The parasite viability
was determined using XTT proliferation Kit.
The Syrian flora contains several species belonging to the genus Inula, but, to our knowledge, no integrated scientific study has been published on the types of the species and the type of glutinous species in particular. Therefore, it was necessary to shed light on this important species spread in the Syrian environment.
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