In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
That study was based on a BLAST search analysis performed against the
complete genome sequences using as a query sequence some of the PSA protein
sequences reported in the literature. In our study, we have first isolated,
cultivated, typed and str
ain of L. major. Then we have investigated
experimentally the complete list of PSA genes coding for membrane proteins in
its genome, using PCR approach based on specific primers. In conclusion, we
have detected the physical presence of 22 different genes coding for membrane
proteins in the genome of Syrian strain of L. major.
In this study the total of ٤١٥ cases of cutaneous leishmaniasis was evaluated
from Dermatology hospital and General clinic in Damascus from February
٢٠٠١ to February ٢٠٠٢. Most of these cases was reported in Damascus and its
suburb, and when we ma
de the direct microscopic test, amastigotes were
detectable in ٢٧١ cases, and the positive cases was increased to ٣٦٤ cases by
realizing the parasite culture into diphasic N. N. N. media, which confirm the
diagnosis.
