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Register a new userDetection of enterotoxin in staphylococcus aureus
الكشف عن الذيفانات المعوية في العنقوديات الذهبية
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أجري البحث على 450 عينة ( 150 عينة لحم صدر – 150 عينة لحم فخد – 150 عينة لحم جناح ) ، جمعت من محلات بيع لحم الدجاج المنتشرة في مدينة حماة للكشف عن تلوثها بالعنقوديات، حيث أظهرت نتائج العزل الجرثومي و الاختبارات الكيمياحيوية وجود 407 عينة إيجابية للعنقوديات منها 73 عزلة ( 16.2 % ) من العنقوديات الذهبية و 334 عزلة ( 83.8 % ) من العنقوديات سالبة المخثراز. كما أظهرت نتائج اختبار تفاعل البوليميراز المتسلسل المتعدد Multiplex PCR على 73 عزلة من العنقوديات الذهبية، وجود 37 ( 50.7% ) عزلة تحتوي على الأقل على مورث واحد مرمز للذيفانات المعوية, و ان المورث sea هو الأكثر مصادفة.
This study were conducted on 450 samples of raw chicken meat ( 150 samples of thighs- 150 samples of breasts -150 samples of wings ) were collected from retail market in Hama city, to detect contamination of staphylococcus. Bacteriological and biochemical tests results showed 407 samples positive for staphylococcus . contained S. aureus 73 (16.2% ) isolated and coagulase-negative Staphylococcus 334 (83.8%) isolated out of total samples. The results of Multiplex PCR test conducted on S. aureus isolates, showed that 37(50.7%) isolates harbored at least one enterotoxin gene, with sea being the most frequently encountered ones.
References used
Al-Khafaji, Marwa Hameed Mtashar, 2013 Detection of Enterotoxins Genes in Staphylococci Isolated from Milk and Cheese, College of Science .J. University of Baghdad
ALVAREZ,A, M., CAPITA, R., ALONSO-CALLEJA, C., MOREONO, B. AND GARCIA-FERNANDEZ, M. C. 2002. Microbiological quality of retail chicken by-products in Spain. Meat Science 62:45-50
AKBAR,A ;ANAL, A, K (2013) PREVALENCE AND ANTIBIOGRAM STUDY OF SALMONELLA AND STAPHYLOCOCCUS AUREUS INPOULTRY MEAT , ASIAN PAC J TROP BIOMED, 3(2): 163-168
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This study were conducted on 450 samples of raw chicken meat
( 150 samples of thighs- 150 samples of breasts -150 samples of
wings ) were collected from retail marketin Hama city,to detect
contamination of staphylococcus.
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In August 2015 the collected leaf of Arctostaphylos uva-ursi were
gathered. Then the volatile oil from fresh samples was extracted
with n-hexane and other samples with steam distillation.
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