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Register a new userComparative btween traditional and modern methods that using in classification Saccharomyces cerevisiae which isolated from apple juice concentrate
مقارنة بين الطرائق التقليدية و الحديثة في تشخيص خميرة الخبز المعزولة من مركزات عصير التفاح
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Aِl-Baath University ورقة بحثية
Publication date
2017
fields
Food Sciences
and research's language is
العربية
Created by
Shamra Editor
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نفذ هذا البحث في مخابر قسم علوم الأغذية بكلية الزراعة بجامعة دمشق، و بمخبر الميكروبيولوجيا و المناعيات في هيئة الطاقة الذرية، حيث أخذ 20 كغ من مركز عصير التفاح بتركيز 70 % من معمل عصير الجبل الطبيعي في محافظة السويداء و منه حضر كل من التركيزيين 35%-15% بإضافة الماء المقطر و المعقم حسب طريقة بيرسون.
This research was conducted at the Department of Food Science, Faculty of Agriculture and National commission for posterity energy. Twenty kg of apple juice concentrate (70%) were tooked from company of Natural Aljabal Juice from AL-suidaa Governorate and prarerd two concentarate (15% and 35%) by using distilled –sterlization water according to pirson .
References used
Barnett, j.,A, Payne,R.,W,andYarrow,D. (2000). Yeasts: characteristics and identification.3 rd edition. Cambridge.university press .England
Bentley, R. W., Leigh, J. A., and Collins M. D. (1991). ”Intrageneric structure of Streptococcus based on comparative analysis of small-subunit rRNA sequences” Int. J. Syst. Bacteriol. (41):487-494
CAB International, (2005). Crop Protection Compendium. Wallingford, UK
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Twenty kg of apple juice concentrate (70%) were tooked from company
of Natural Aljabal Juice from AL-suidaa Governorate and prarerd two
concentarate (15 and 35%) by using distilled –sterlization water . these
samples stoered at (4-20-30-40)ºc for
3 months, chemical and microbial
tests were assayed in the every month average triple to every concentrate
and all different temperatures and recored the average, The chemical
analysis results show: The acidity relaise in all samples that storage at 20
ºc beacause it is agreeable for oraganisms to grow.
In this research, the
antioxidant capacity(DPPH method), and total phenolic content (assayed
by Folin-Ciocalteu method), ,flavonoids, anthocyanins (pH differential
method) and ascorbic acids(2,6-dichlorophenol indophenol) in three fruit
tissues (
peel, pulp and whole fruit), of four apple cultivars commonly in
Syria(Red Delicious, Rome Beauty, Starking Delicious, Golden
Delicious), were studied.
This study was conducted at the laboratories of the Faculty of Agriculture,
Damascus University and the National Commission of Biotechnology in 2012 to
investigate he possibility of Production of bioethanol using local isolate of
Saccharomyces cer
evisiae from raw sugar refining molasses and to determine
the effect of different temperatures, pH and concentration of Dry matter (Brix
degree) in molasses on the amount of ethanol produced. A local isolate (R1) of
Saccharomyces cerevisiae selected previously due to its superiority in ethanol
production was taken from wine brewing residues and exposed to different
temperatures (20, 25, 30 and 35OC), pH (4, 4.5, 5 and 5.5) and the Brix degrees
(16, 20, 24 and 28%) during fermentation.
Results revealed that the best amount of ethanol was produced at
temperature of 30 º C, pH 5, and Brix degree in molasses 16%.
Forty-five Bacillus isolates were obtained from different foods (spices,
grains, dried milk), the isolates were identified using traditional method from
Bergey's manual based on morphological tests (gram staining, spores staining),
physiological t
ests (optimum growth temperature, pH, NaCl concentration) and
biochemical tests (Voges-Proskauer, gelatin hydrolysis, esculin hydrolysis, β-
Galactosidase test, Lysine decarboxylase and Ornithine decarboxylase,
production of acid from different sugars). Bacterial suspensions were prepared
from the isolates and FTIR spectrums were obtained using ATR unit, which
consists of ZnSe prism, by scanning at the range of 4000-400 cm-1. Results
revealed that the isolates represented 5 species of Bacillus (B. pumilus, B.
subtilis, B. lentus, B. megaterium, B. cereus) and the potential of Fourier
transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR)
method as a reliable method for discrimination among Bacillus isolates, which
is accurate, rapid, easy to apply and cheap.
The study investigated the survival ability of probiotic Lactobacillus
casei in apple juice to produce functional juice ,as well as,
optimizing the growth conditions of L.casei in apple juice including
concentration, temperature ,inoculum size usi
ng Response Surface
Methodology as statistical method.
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