In our study, the
presence and expression of P27 gene in L.tropica evaluated in
promastigotes by PCR and RT-PCR using specific primer pairs
that manually designed after determine the consensus sequence of
forward and reverse region of primers bet
ween species of
Leishmania by using MUSCLE (Multiple Sequence Comparison by
Log- Expectation) tool for alignment. The results proved the
presence of P27 gene in Leishmania tropica, beside presence the
mRNA of P27 gene that confirm that P27 expressed in
promastigote form of L.tropica
the leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania Tropica parasite , the hardness of
controlling the reservoirs of parasites, and there is no specific ,safe
active therapy. A Heat shock protein 20 HSP2
0 belongs to the
small HSP (sHSP) family.
This study evaluated the in vitro
antileishmanial activity of some Phenolic compounds including:
Ferulic acid, Mehtyl Ferulate, Ethyl Ferulate, and Sinapic acid
against leishmanial tropica promastigotes. The parasite viability
was determined using XTT proliferation Kit.
The leishmaniasis is a serious health problem in Syria, due to the
wide spreading of Leishmania tropica parasite , the difficulty of
controlling the reservoirs of parasites, and there is no specific ,safe
and active therapy .Due to lack of studies
of Leishmania tropica
genome , as initial step we detect the presence of SW3 gene.
The Syrian flora contains several species belonging to the genus Inula, but, to our knowledge, no integrated scientific study has been published on the types of the species and the type of glutinous species in particular. Therefore, it was necessary to shed light on this important species spread in the Syrian environment.
