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Register a new userتقييم طريقة Multiplex PCR لكشف نسخ الرنا المرسال للجين المندمج BCR-AbL لدى مرضى الابيضاض النقوي المزمن
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Damascus University رسالة ماجستير
Publication date
2016
fields
Pharmacy
and research's language is
العربية
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Shamra Editor
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تقييم طريقة Multiplex PCR لكشف نسخ الرنا المرسال للجين المندمج BCR-AbL لدى مرضى الابيضاض النقوي المزمن
No English abstract
References used
Advane As, Pendergast AM. Bcr-Abl variants: biological and clinical aspects . Leuk Res. 2002
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Serglycin is an intracellular proteoglycan in hematopoietic cells. It
has been studied in normal and tumor hematopoietic cell, so it was
suggested to have an important role in immunity and cancers as leukemia.
The challenge to make a diagnosis for
acute leukemia and to
differentiate between myeloid or lymphoblastic leukemia, is the reason
we go to assay concentration of serglyc in normal people and
patient with acute myeloid leukemia and acute lymphoblastic leukemia
from children and adult at hematology department in the
main hospitals in Damascus ( Pediatric’shopital, Almouasat and Al
asaad hospital) before beginning any kind of treatment and studying
the relation with white blood cells count. We found clear difference
between the concentration in patient with acute leukemia and normal
people. There was a high level of this marker in paint with
AML in contrast with ALL patients. We found a relation between
concentration of serglycin and the count of white blood cells.
Chronic Periodontitis is accompanied with subacute systemic inflammatory
responses and a procoagulant state which manifests in changes of some serological vascular thrombotic
markers levels. This study aimed to evaluate several vascular thrombotic markers levels in patients with
chronic periodontitis.
Several studies and reports have indicated the prevalence of hepatitis D virus
infection in many parts of the world at different rates , Ranging from areas of
epidemiological and areas with a low prevalence of it.
Till now there is no study in Syr
ia shows us the prevalence of viruse Delta in chronic
hepatitis B patients .
The aim of our study was to determine the current prevalence of hepatitis D in
chronic hepatitis B patients . And to take appropriate measures in dealing with these
patients due to the increased risk of liver disease in the event of injury by it .
77 patients with chronic hepatitis B certainly proved with PCR analysis
25 women and 52 men visiting the center of hepatitis in Latakia were tested for
antibody to hepatitis D virus (anti-HDV) by ELISA test .
The results indicate a positive antibody in three patients (women and 2 men) and the
prevalence was 3.8% , which point to a moderate prevalence of hepatitis D comparing with
neighboring countries.
In the present study, we measured DNMT3A levels, the
methylation enzyme, in blood samples from Syrian AML patients
and normal individuals using real-time quantitative polymerase
chain reaction, in order to determine the roles of DNMT3A in the
development of leukemia.
Gene regulation by microRNAs (miRNAs) is one of the most important regulatory
networks which control normal hematopoiesis. Disturbances in miRNAs levels lead to
proliferation diseases including leukemogenesis. MicroRNAs is a major topic of many
ca
ncer researches performed to discover noninvasive biomarkers used for diagnosis,
prognosis, and optimization of clinical decision.
This study, the first to be performed in Syria, aimed at monitoring miR-155 levels
compared to a normalizer gene RNU6-2 by quantitative reverse transcriptase -PCR (qRTPCR)
in a sample of newly diagnosed untreated AML patients at several hospitals in
Damascus in comparison with healthy controls. Changes in miR-155 gene expression
levels were calculated in patients and controls using the 2-ΔCt method.
The most important finding was the association of low and very high miR-155 levels
with poor prognosis reflected in failure to accomplish complete remission and high
mortality. In addition, high miR-155 levels were associated with M4 AML subtype,
although with large variance among patients. We hope our preliminary study pave the road
for many future research studies related to the applicability of microRNAs in supporting
diagnosis, predicting prognosis, and enhancing the personalized therapies which deal with
patients as individual cases.
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