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This study was carried out on 90 broiler chicks which divided into three groups. The first group has been given 0.75 g/L potassium nitrate and the second group was given 1 g/L potassium nitrate with drinking water for 30 days, While the third group w as regarded as a control. The birds were reared in same keeping house and feeded with the same ration without any additives and no vaccinations were applied. All birds were sacrificed and examined by naked eye. Then the thymus gland was fixed by formalin and stained microscopic sections were examined by microscope. The histological changes in the birds which dosed by 1 g/L and 0.75 g/L of nitrate were similar and the observed changes were the small size of cortex in some lobules, and the cortex were separated from bulb with increase the number of Hassall bodies in the bulb region, also there were vacuoles and reticular cells in different stages of degeneration and edema in the connective tissues in comparison with control group. These results confirmed that nitrate had toxic effects on the tissues of thymus, and as a sequence reduced the number of T cells which lead to decrease the resistance and Immune response of the birds and retarded the growth rate.
This study aimed to determine the effect of anticoccidial drug (Sulphaquinoxaline), that deployed in poultry farms in The Syrian Arab Republic, on some blood parameters, of broiler chickens for the commercial type(Ross), that available in Syria. We u sed drug being tested by two doses(therapeutic dose and a double therapeutic dose), as the following: (125, 250)ppm, respectively .The experience birds that braved /120/ chick at aged one day were divided to three equal distribution groups (Control, group(1), Sulphaquinoxaline, group(2) at concentration(125)ppm, Salafaquinoxaline, group(3) at concentration(250)ppm). The experience period continued for(45) days. The anticoccidial drug were presented continuously with fodder, and the blood samples were collected three times during this period. The blood samples were taken from broilers at the age(15 - 30 - 45) days. 10 samples were taken of each group from indentified three groups, then lab tests were done on the blood picture. These Tests included determination of the values of total protein, albumin and Globulin in blood serum, and also it included determination the values of some mineral elements in blood serum. These elements contained[Ca- P- Mg ] in serum.
This study aimed to determine the effect of antifungal drug (copper Sulphate) on blood picture of broiler chickens Vaccinated with Newcastle Disease Vaccine, We used the drug being tested by two doses: (50, 100) ppm from the age of 7 till 45 Days. We used /150/ chick were divided into three equal groups.
Two hundred fecal samples were randomly collected from various aged broiler flocks farms, which suffered from diarrhea or respiratory problem or both in Tartous and Latakia Provinces of Syria. This study demonstrated the infection of the broiler flocks with Cryptosporidium in Tartous Province of Syria with a rate of 5.6% only. The results of tests based on morphology and size of Cryptosporidium oocysts showed that the parasite is probably C. baileyi which ranged in size about (6 μm X 4.5 μm).
A reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to detect infectious bronchitis virus (IBV) in the commercial broiler flocks in Syria. 50 tissue samples were taken from tracheal tissue, trachea and kidney of the broil ers suspected of infectious bronchitis (IB) from different governorates of Syria i.e. Latakia, Tartous, Hama and Damascus countryside. RNA was extracted directly from the tissue samples and then RNA was converted to cDNA by RT-PCR technology; PCR reaction and Nested PCR interaction were carried out sequentially. The primers used in the RT-PCR reaction were selected from the S1 gene (spike), where mutations of the virus genome were concentrated in this region (the hypervariable region). Some positive samples (10) were injected at an age of 9-11 days old SPFEE-specific pathogen free embryonated eggs according to the methods adopted in virology. This research was carried out at the laboratories of Latakia Research Center, General Commission for Scientific Agricultural Research GCSAR, in cooperation with the PCR laboratory at the Faculty of Veterinary Medicine in Hama. The results showed the existence of 37 positive case for RT-PCR (74%), and the infectious embryos showed clear and characteristic anatomical lesions of the infectious bronchitis virus after 5-6 days post injection, delayed and undeveloped fetal (dwarfism), fingertip entanglement and hemorrhage compared with the negative control. The results also showed the sensitivity and speed of the RT-PCR test in the detection of the IBV virus.
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