This research was conducated on Ceratonia siliqua L. in vitro to study the effect of
some growth regulators in both multiplication and rooting stage. And determine the best
concentrations wich lead the highest rate of multiplication (interms of number of shoots
and length), and the best rooting (interms of percentage of rooting, number of roots and
length).
This study was conducted during 2012-2013 in order to efficiently micropropagate
viburnum (Viburnum tinus) bushes using tissue culture techniques. The shoots were
cultured on a Murashige and Skoog medium supplemented with 30 gl-1 sucrose, 7 gl-1 ag
ar
- agar, citric acid as an anti-oxidant (300 mgl-1), and different concentrations of benzyl
amino purine and Naphthalene Acetic Acid. A media of Murashige and Skoog was used
for laboratory rooting after reducing the major mineral elements to the half, reducing the
sucrose to 20 gl-1, and addingindole-3-butyric acid (IBA) of different concentrations (0,
0.5, 1, 1.5 mgl-1). The results showed that it is necessary to have auxin and cytokinin in
culture to improve the value of open buds and the number of shoots per initial explants.
The concentrations 0.25 mgl-1 from NAA with 1 mgl-1 from BAP gave the highest value of
open buds (93.33%) and the maximum number of shoot per initial explants (1.57). To
improve the number and length of the shoots produced, the solution mineral (MS) was
replaced with another: media woody plants (WPM) which gave better elongation for the
resulting growths (3.21 cm) and a better number of shoots (2.72 Growth/explant)
compared to the media (MS) using the same compatibility hormone (0.25 mgl-1 of NAA
and 1 mgl-1 of BAP).
The results also show that the highest percentage of rooting reached (84.44%) with
(0.5 mgl-1) IBA which was better than (1.5 mgl-1) IBA and better than the treatment of the
control. Results also showed that the best medium for the length and number of roots
formed was (2.7cm, 3.82root) when the concentration was (0.5 mgl-1) surpassing the
control. The success rate of the acclimatization of the resulting laboratory plantlets under
glasshouse conditions reached (73.33%) one month and a half after the transplanting.
This research was conducted to detect the morphological and molecular
differences between golden henbane (Hyoscyamus aureus L) collected from
south Syria. Seeds were collected from six locations, then sterilized and grown
In Vitro. Grown plants we
re described for some morphological characters. The
molecular characterization was carried out using the method of Inter Simple
Sequence Repeats (ISSR) to detect genetic variations. The plants showed
significant (p<0.01) morphological differences among studied locations, Plant
height ranged from 14.97 to 18.97 cm and stem color ranged from red to green.
Trichome density also ranged between medium, high and very high.
Furthermore, significant differences in leaves dimensions were observed
according to the plant geographical location. The main root length ranged from
6.09 to 8.37 cm. Molecular analysis by ISSR produced a total of 56 bands, 49 of
which were polymorphic (87.5%), resulted from using 11 non-specific primers.
A genetic distance dendrogram was drawn. based on the results of genetic
characterization of plant from different locations. The plants collected from
Salkhad separated with a genetic distance of 0.312 while the plants collected
from other locations were grouped together, at the same time, they were
divided into two sub groups, the first group comprised plants collected from
Qalamoun and Dimas regions with a genetic distance of 0.032, whereas sub
group II content Bosra plants which separated clearly from those of Dara' dam
and Wadi Al-Zaidi by a genetic distance of 0.017, These results may indicate a
relationship between genetic characters of this plant species with geographic
distribution.
Experiments were conducted to study effect of radiation and salt levels on plant
regeneration from callus for two cultivars of potato i.e. Riviera and Burren, under
in vitro condition. Results showed that the efficiency of radiation in induced
reg
eneration from callus in Riviera cultivar at salt level of (10 dS m-1) and Burren
cultivar at salt levels (8, 12 dS m-1). For the purpose of making sure inheritance
of salinity tolerant, mutant clones (plants induced from salt tolerant calli, which
planted at salt levels of 8, 10, 12 dS m-1 and plants induced from non salt tolerant
calli, which planted at salt level of 6 dS m-1) and their parental cultivars (Riviera
and Burren) by exposing to salt stress conditions and comparing them with control
(6 dS m-1). Results revealed that the lowest percentage of reduction in plant height,
number of nods per plant and tuberization were observed in salt tolerant mutants.
Plant height and number of nodes/plant can be considered as selective morphological
markers for in vitro salt tolerance .
