Potato (Solanum tuberosum L.) is among the most
economically important crops world-wide and in Syria
as well. It is highly responsive to different tissue culture
techniques.
In vitro culture of potato cv. 'Draga' (mid-early) was
established thro
ugh meristem tip culture.
The effect of different culture medias (water, MS solid, MS 1/2 solid) on germination
and growth of heliotropiun hirsutissimum Grauer. in vitro was studied with different
concentrations of gibberellic acid (0.001-0.01-0.1 and 1 mg/l).
The results
showed that best germination rate (80.5%) and root (20mm) and shoot
(35mm) growth were obtained in MS solid medium with 0.1mg/l GA3.
Addition of 0.1mg/l GA3 to MS1/2 solid medium improved germination rate
(75.44%), root (19mm), and shoot (24mm)growth.
When GA3 (0.1 mg/l) was added to water medium, germination rate reached
(65.33%), as well as root and shoot growth (12.66mm and20mm) alternatively after 2
weeks of planting.
Seedling of MS solid medium were transferred into pots contained torp medium to
adapting them with outside environment, and then surviving their growth until maturity
after 4 weeks.
