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Register a new userStudy the effect of some growth regulators and number of subcultures on micropropagation of medicinal plant Ziziphora canescens Benth
دراسة تأثير بعض منظمات النمو و عدد الزراعات الثانوية في الإكثار الدقيق لنبات الزيزيفورا الطبي Ziziphora canescens Benth
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يعد النوع Ziziphora clinopodioides Lam) Ziziphora canescens) من النباتات الطبية المهمة في سورية لما يتمتع به من خصائص طبية مهمة كمضادات حيوية و منكهات و توابل في الأطعمة المختلفة. و نظراً إلى أهمية هذه النباتات و خاصة في الطب الشعبي في بعض المناطق (القلمون) من ناحية، و لتراجع مساحات انتشاره التي قد تؤدي بالنهاية إلى انقراضه من ناحية أخرى، فقد وضع بروتوكول للإكثار السريع باستعمال تقانة زراعة الأنسجة النباتية؛ و ذلك انطلاقاً من البراعم الجانبية و القيمة على وسط MS المزود بأنواع و تراكيز مختلفة من منظمات النمو النباتية.
Ziziphora canescens is a species of important medicinal plants in Syria due to its medicinal properties as antibiotic, flavors and spices in various foods. This plant is important, especially in folk medicine in some areas (Kalamoon) on the one hand, and retreat of its spread which may lead eventually to its extinction on the other hand, so a protocol for rapid micropropagation has been developing by using lateral and apical buds on nutrient media MS supplemented with different types and concentrations of plant growth regulators.
References used
Al-Sane, K. O. Shibli, R. A. Freihat, N. M. and Hammouri, M. K., 2005. Cell Suspension Culture and Secondary Metabolites Production in African Violet (Saintpaulia ionantha Wendl.). Jordan Journal of Agricultural Sciences.1(1): 84-92
Anzabi, Y. Aghdam, B. V. Makoui, H. M. Anvarian, M. and Mousavinia, N. M., 2013. Evaluation of antibacterial Properties of Edible Oils and Extracts of A Native Plant, Ziziphora Clinopodioides (Mountains’ Kakoty), on Bacteria Isolated From Urinary Tract Infections. Life Science Journal. 10: 121-127
Baser, K. H.C. Sezik, E. and Tumen, G., 1991. Composition of the essential oil of Ziziphora clinopodioides Lam. J. Essential oil Res. 3: 237-239
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Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus
communis L.) grown in the field under natural conditions at Damascus
countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected
by 70% Ethanol an
d Clorox containing 5.25 % Sodium Hypoclorite
with a drop of Tween 20 for different periods and concentrations before being
placed onto MS basal medium. Established cultures were then transferred onto
MS basal medium containing a combination of growth regulators at different
concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM
or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of
12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44
μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
This research was conducated on Ceratonia siliqua L. in vitro to study the effect of
some growth regulators in both multiplication and rooting stage. And determine the best
concentrations wich lead the highest rate of multiplication (interms of number of shoots
and length), and the best rooting (interms of percentage of rooting, number of roots and
length).
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
Investigate studied the activity of some enzymes in zea maize plant seedlings
cells, (Ghota 82) under the influence of phytohormone Auxin & growth
regulators 2,4-D and IBA.
Maize plant seeds were germinated in water medium for six days in
darknes
s and temperature of 260C.
Then stem cells of the developing seedling were incupated in darkness with
water or in different solutions of auxin, 2,4-D & IBA (50 mg/l) for 20 houre in
30 0C. Then magared the activity of α-Amylase, RNAase, Catalayse, Guaiacol
Peroxidase, Ascorbate oxidase in the stem cells
The results showed that the incupation has defferent effects.on hydrolysis
and oxidative enzymes in zea maize plant seedlings cells.
This research aims to study the proteins transformation in zea maize plant
seedlings cells, (GHOTA 82) under the influence of phyto hormone Auxin &
grothregulators 2,4-D and IBA.
Maize plant seeds were germinated in water medium for six days in
d
arkness and temperature of 25 ºC.
The quantity different groups of proteins, were analyzed in the stem cells of
the developing seedling mesochotyles after incupation in darkness water and in
different solutions of auxin, 2, 4D & IBA (in concentration 50 mg/l) for 20
hours in 26 ºC.
The results showed that had inhibition effects on hydrolysis of protein
groups in zea maize plant seedlings cells.
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