In this
search we characterized 8 double purposes olive varieties (Mawi
Istanbuli, Khilkhali Khishen, Dan, Mniekri, Doaibli, Khilkhali
Saghir, Karamani Modabal, Sourani), which cultivated in ACSAD
Gene bank in Gellin by ISSR molecular markers to
determine the
relationship between these varieties, we noticed that these varieties
are so close to each others depending on quantitive traits, which
ranged from 60% and 85%, it was noticed that Mneikri was apart
from the other varieties in its traits, polymorphic rate was 92.94%.
Seven plant samples were collected from some locations of Syrian juniper
(Juniperus drupacea Labill.) in Syria for molecular characterization and to
determine the genetic relationships between them using ISSR technique (Inter
Simple Sequence Repea
ts). Twenty three ISSR primers were used for this
purpose, twelve primers showed polymorphism between studied samples and
gave 89 bands, with polymorphism percentage of 95.5%. The band number
resulted from each primer ranged between 4 bands for primers ISSR5 and
ISSR9, and 12 bands for the primer ISSR1, with an average of 7.42 bands per
primer. The minimum polymorphic percentage was 25% for primer ISSR9,
and the maximum polymorphic percentage was 100% for the all primers
except the primer ISSR9. The study showed correlation between the genetically
converged samples and the collection sites (geographic correlation), the highest
genetic relationship (93%) was within Latakia samples (Komat Alnabi Yonis –
Jobet Bergal) and the lowest genetic relationship (42%) was between samples
from Hamah (Jeb Alahmer) and Latakia (Almakamat) which refers to high
genetic variation. The cluster analysis showed that the samples from nearby
locations were gathered.
This research was conducted to detect the morphological and molecular
differences between golden henbane (Hyoscyamus aureus L) collected from
south Syria. Seeds were collected from six locations, then sterilized and grown
In Vitro. Grown plants we
re described for some morphological characters. The
molecular characterization was carried out using the method of Inter Simple
Sequence Repeats (ISSR) to detect genetic variations. The plants showed
significant (p<0.01) morphological differences among studied locations, Plant
height ranged from 14.97 to 18.97 cm and stem color ranged from red to green.
Trichome density also ranged between medium, high and very high.
Furthermore, significant differences in leaves dimensions were observed
according to the plant geographical location. The main root length ranged from
6.09 to 8.37 cm. Molecular analysis by ISSR produced a total of 56 bands, 49 of
which were polymorphic (87.5%), resulted from using 11 non-specific primers.
A genetic distance dendrogram was drawn. based on the results of genetic
characterization of plant from different locations. The plants collected from
Salkhad separated with a genetic distance of 0.312 while the plants collected
from other locations were grouped together, at the same time, they were
divided into two sub groups, the first group comprised plants collected from
Qalamoun and Dimas regions with a genetic distance of 0.032, whereas sub
group II content Bosra plants which separated clearly from those of Dara' dam
and Wadi Al-Zaidi by a genetic distance of 0.017, These results may indicate a
relationship between genetic characters of this plant species with geographic
distribution.
The research was carried out in the biotechnology Laboratory at the
Faculty of Agriculture, Damascus University, in cooperation with the General
Commission For Scientific Agricultural Research (GCSAR), during the year
2012. Eleven genotypes of cul
tivated chickpea were planted to determine their
genetic diversity and the degree of genetic similarity using the ISSR technique.
16 primers were used for this purpose. Results indicated that Nine primers
proved their effectiveness in showing polymorphism among the genotypes
examined and the primers gave a total of 91 alleles with a polymorphic
percentage of 93.4%. The number of bands for each primer varied from a
minimum of 2 bands for the primers ISSR7-ISSR13 to a maximum of 16 bands
for the primer ISSR11, with an average of 9.1 bands per a primer. The results
also showed that the degree of genetic similarity ranged between 46 and 78%,
where the highest was 78% between the genotypes of Afghanistan – Morocco,
then 77% between the genotypes Tunisia-Afghanistan and Tunisia- Iran and
followed by Spain- Iran and Spain- Pakistan (50%) and the lowest was 46%
between genotypes Afghanistan- Pakistan.
This investigation was carried out at the Laboratory of Biotechnology at
General Commission for Scientific Agricultural Research (GCSAR), during the
season 2010-2011. The aim of this research was to study the genetic diversity
among twenty individ
ual plants of seven species and to determine the degree of
genetic similarity using the technique ISSR (Inter Simple Sequence Repeats)
and 10 primers were used for this purpose. All primers proved their
effectiveness in showing polymorphism between the studied species, primers
gave a total 195 allele with a polymorphic percentage 100%. The number of
bands for each primer varied from a minimum of 12 bands for the primer
(ISSR-4) to a maximum of 27 bands for the primer (ISSR-862) in an average of
19.5 bands for each primer, cluster analysis and Dendrogram showed the
highest degree of genetic similarity between accession A.leucoclada1 and
A.leucoclada2 (0.64), while it was low between species A.leucoclada3 and
A.glauca2 (0.10). Results showed vast genetic diversity among the studied
species.
This research was carried out in molecular biology laboratory
faculty of agriculture /Damascus University during the agriculture
season 2015-2016, to study the genetic diversity and determine the
degree of genetic similarity among eleven 11 cultiv
ated vetch
(Aleppo, Idleb, Kamishly, Hassekeh, Tartous, Lattakia, Homs,
Hama, Sweida, Damascus,Daraa) by using the technique ISSR.
