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Effect of media culture composition on In Vitro germination and growth of seeds of Heliotropium hirsutissimum Grauer

تأثيــــر تركيب الوسط المغذي في إنبـــات و نمو نبات الزريقاء اللبديـــــة Heliotropium hirsutissimium Grauer المزروعـــــة مخبريـــــاً فـــي الأنابيب

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 Publication date 2016
  fields Biology
and research's language is العربية
 Created by Shamra Editor




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The effect of different culture medias (water, MS solid, MS 1/2 solid) on germination and growth of heliotropiun hirsutissimum Grauer. in vitro was studied with different concentrations of gibberellic acid (0.001-0.01-0.1 and 1 mg/l). The results showed that best germination rate (80.5%) and root (20mm) and shoot (35mm) growth were obtained in MS solid medium with 0.1mg/l GA3. Addition of 0.1mg/l GA3 to MS1/2 solid medium improved germination rate (75.44%), root (19mm), and shoot (24mm)growth. When GA3 (0.1 mg/l) was added to water medium, germination rate reached (65.33%), as well as root and shoot growth (12.66mm and20mm) alternatively after 2 weeks of planting. Seedling of MS solid medium were transferred into pots contained torp medium to adapting them with outside environment, and then surviving their growth until maturity after 4 weeks.


Artificial intelligence review:
Research summary
تناول البحث دراسة تأثير تركيب الوسط المغذي على إنبات ونمو بذور نبات الزريقاء اللبدية (Heliotropium hirsutissimium Grauer) المزروعة مخبرياً. تم استخدام ثلاثة أوساط مغذية مختلفة (الماء، وسط MS الجامد، وسط 1/2 MS الجامد) مع تراكيز مختلفة من منظم نمو الجبريلين (0.001-0.01-0.1-1 مغ/ل). أظهرت النتائج أن الوسط MS الجامد بتركيز 0.1 مغ/ل من الجبريلين حقق أعلى نسبة إنبات بلغت 80.5%، وأفضل نمو للجذور والسيقان. كما أظهرت النتائج تحسن نسبة الإنبات والنمو عند إضافة الجبريلين بتركيز 0.1 مغ/ل إلى وسط 1/2 MS الجامد والماء. تم نقل بعض البادرات الناتجة إلى أصص تحتوي على تربة مغذية وتمت أقلمتها حتى الحصول على نباتات بحالة خضرية جيدة بعد شهر من الزمن.
Critical review
دراسة نقدية: يعتبر البحث مهماً في مجال زراعة الأنسجة النباتية، حيث يقدم معلومات قيمة حول تأثير الأوساط المغذية ومنظم نمو الجبريلين على إنبات ونمو بذور نبات الزريقاء اللبدية. ومع ذلك، يمكن تحسين البحث من خلال تضمين تجارب إضافية باستخدام منظمات نمو أخرى، وكذلك دراسة تأثير العوامل البيئية المختلفة مثل درجة الحرارة والرطوبة على الإنبات والنمو. كما يمكن توسيع الدراسة لتشمل أنواع أخرى من نباتات الفصيلة البوراجينية للاستفادة من النتائج في تطبيقات زراعية وطبية أوسع.
Questions related to the research
  1. ما هو الهدف الرئيسي من البحث؟

    الهدف الرئيسي هو دراسة تأثير تركيب الوسط المغذي ومنظم نمو الجبريلين على إنبات ونمو بذور نبات الزريقاء اللبدية المزروعة مخبرياً.

  2. ما هي الأوساط المغذية التي تم استخدامها في الدراسة؟

    تم استخدام ثلاثة أوساط مغذية: الماء، وسط MS الجامد، وسط 1/2 MS الجامد.

  3. ما هي أفضل نسبة إنبات تم تحقيقها في الدراسة؟

    أفضل نسبة إنبات تم تحقيقها كانت 80.5% في الوسط MS الجامد بتركيز 0.1 مغ/ل من الجبريلين.

  4. ما هي التوصيات التي اقترحها الباحثون بناءً على نتائج الدراسة؟

    اقترح الباحثون دراسة تأثير تراكيز منظمات نمو أخرى، وإجراء دراسات معمقة على جنس Heliotropium للاستفادة من خلاصاته في التطبيقات الطبية.


References used
AL-SELAWY, R.L.A. Response of growth and yield of some Rice cultivars to the seed Enhancement. P.H.D. Dissertation. Field crops DEPT., College of agriculture, University of Baghdad, Iraq, 2011, 106
ALWAHIBI, M.; BUKHARY, N. Anatomical study of four species of heliotropium L. (Boraginaveae) from Saudi Arabia, African journal of plant science, 7(1), Saudi Arabia, 2013, 35-42
BAGADEKAR, A.N.; JAYARAJ, M. In vitro rhizogenesis from leaf and stem callus of heliotropium indicum, L.- medicinal herb, international journal of plant, animal and environmental sciences, 1(2), India, 2011, 1-5
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