تــم فــــي هــــــــــذا البحــث دراســــــة تأثيـــــر تركيـــــــب الوســـط المغــــــذي فــــــــــي إنبــات و نمـــــــــــو بـــــــــــــذور النـــــــــــــــــوع Heliotropium hirsutissimium Grauer (نبات الزريقاء اللبدية) المزروعة مخبرياً في الأنابيب In vitro.
حيث تم استخدام الأوساط التالية (الماء، وسط MS جامد Murashige & Skoog، وسط 1/2 MS جامد) مع تراكيز مختلفة من منظم نمو الجبريلين GA3 1- 0.1- 0.01- 0.001)مغ/ل) أو بدونه. أظهرت النتائج تفوق الوسط MS الجامد بوجود الجبريلين 0.1 مغ/ل على باقي التراكيز و الأوساط الأخرى، حيث سجل أعلى نسبة إنبات بمعدل 80.5%، و بمتوسط طول جذر بلغ 20 مم، و متوسط طول ساق 35 مم بعد أسبوعين من الزراعة. كما تحسنت نسبة الإنبات في الوسط 1/2 MS الجامد مضافاً إليه الجبريلين بتركيز 0.1 مغ/ل، حيث بلغت 75.44%، و سجل متوسط طول الجذر 19مم، و الساق 24 مم. كما أدى إضافة الجبريلين 0.1 مغ/ل إلى الماء، إلى الحصول على إنبات 65.33% بعد أسبوعين من الزراعة، و بمتوسط طول جذر 12.66 مم، و متوسط طول ساق 20 مم. زرعت بعض البادرات الناتجة من أفضل وسط في أصص تحوي تربة مغذية (تورب) و تمت أقلمتها حتى الحصول على نباتات بحالة خضرية جيدة أخذت نتائجها بعد شهر من الزمن.
The effect of different culture medias (water, MS solid, MS 1/2 solid) on germination
and growth of heliotropiun hirsutissimum Grauer. in vitro was studied with different
concentrations of gibberellic acid (0.001-0.01-0.1 and 1 mg/l).
The results showed that best germination rate (80.5%) and root (20mm) and shoot
(35mm) growth were obtained in MS solid medium with 0.1mg/l GA3.
Addition of 0.1mg/l GA3 to MS1/2 solid medium improved germination rate
(75.44%), root (19mm), and shoot (24mm)growth.
When GA3 (0.1 mg/l) was added to water medium, germination rate reached
(65.33%), as well as root and shoot growth (12.66mm and20mm) alternatively after 2
weeks of planting.
Seedling of MS solid medium were transferred into pots contained torp medium to
adapting them with outside environment, and then surviving their growth until maturity
after 4 weeks.
References used
AL-SELAWY, R.L.A. Response of growth and yield of some Rice cultivars to the seed Enhancement. P.H.D. Dissertation. Field crops DEPT., College of agriculture, University of Baghdad, Iraq, 2011, 106
ALWAHIBI, M.; BUKHARY, N. Anatomical study of four species of heliotropium L. (Boraginaveae) from Saudi Arabia, African journal of plant science, 7(1), Saudi Arabia, 2013, 35-42
BAGADEKAR, A.N.; JAYARAJ, M. In vitro rhizogenesis from leaf and stem callus of heliotropium indicum, L.- medicinal herb, international journal of plant, animal and environmental sciences, 1(2), India, 2011, 1-5
The investigation was carried out during 2011-2012 at the laboratories of Plant
Protection Dep., Faculty of Agriculture, and Chemistry Dep., Damascus
University. The fatty acid composition in the seed and flower of Chinaberry
(Melia azedaracht L.)
The objective of this study is to compare the effect of five different Nacl
concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two
cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile
This study was conducted at the biotechnology laboratory of AnimalWealth
Research Administration, General Commission for Agricultural Research in
order to compare the effect of different media on In vitro maturation (oocytes
matured at 39 C° under
The experiment was carried out to assess the germinability and seedling
growth rate of some treated vegetable seeds (i.e. onion, carrot, and tomato) with
aerated - aqueous solutions.
Seeds were treated for 24 hours with 0.1% of both succinic acid
The objective of this study is to compare the effect of five different Nacl concentration (0,50,100,150,200 mM) on seeds germination and seedling growth of two cultivars of soybean (sb-44,sb-172).
Seeds were germinated in petri dishes with sterile