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Effect of Salinity and Radiation on Regeneration of Two Potato (Solanum tuberosum L.) Genotypes Callus In vitro

تأثير الملوحة و التشعيع في إخلاف صنفين من البطاطا (Solanum tuberosum L.) من الكالّس خارج الجسم الحي

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 Publication date 2018
and research's language is العربية
 Created by Shamra Editor




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Experiments were conducted to study effect of radiation and salt levels on plant regeneration from callus for two cultivars of potato i.e. Riviera and Burren, under in vitro condition. Results showed that the efficiency of radiation in induced regeneration from callus in Riviera cultivar at salt level of (10 dS m-1) and Burren cultivar at salt levels (8, 12 dS m-1). For the purpose of making sure inheritance of salinity tolerant, mutant clones (plants induced from salt tolerant calli, which planted at salt levels of 8, 10, 12 dS m-1 and plants induced from non salt tolerant calli, which planted at salt level of 6 dS m-1) and their parental cultivars (Riviera and Burren) by exposing to salt stress conditions and comparing them with control (6 dS m-1). Results revealed that the lowest percentage of reduction in plant height, number of nods per plant and tuberization were observed in salt tolerant mutants. Plant height and number of nodes/plant can be considered as selective morphological markers for in vitro salt tolerance .


Artificial intelligence review:
Research summary
تناولت هذه الدراسة تأثير الملوحة والتشعيع على إخلاف نباتات البطاطا من الكالَس لصنفي Riviera وBurren خارج الجسم الحي. أظهرت النتائج أن التشعيع كان فعالاً في تحفيز إخلاف النباتات من كالَس الصنف Riviera عند مستوى ملوحة 10 ديسي سيمنز/م، ومن كالَس الصنف Burren عند مستويات ملوحة 8 و12 ديسي سيمنز/م. تم تقييم سلوك السلالات الطافرة المستحدثة من الكالَس المتحمل للملوحة وغير المتحمل للملوحة تحت ظروف الإجهاد الملحي، ومقارنتها مع النباتات الأصلية. أظهرت النتائج أن أقل نسبة انخفاض في ارتفاع النبات وعدد العقد وتكوين الدرينات كانت في السلالات الطافرة المتحملة للملوحة. يمكن اعتماد ارتفاع النبات وعدد العقد/نبات كمعيار انتخابي لتحمل الملوحة خارج الجسم الحي.
Critical review
دراسة نقدية: تعتبر هذه الدراسة مهمة في مجال تحسين تحمل النباتات للملوحة باستخدام تقنيات زراعة الأنسجة والتشعيع. ومع ذلك، يمكن توجيه بعض الانتقادات البناءة. أولاً، كان من الأفضل توسيع نطاق الدراسة لتشمل أصناف أخرى من البطاطا لتحسين تعميم النتائج. ثانياً، لم يتم التطرق بشكل كافٍ إلى الآليات الجزيئية التي تساهم في تحمل الملوحة، مما يحد من فهمنا الكامل للعمليات البيولوجية المعنية. أخيراً، كان من الممكن تحسين تصميم التجارب من خلال زيادة عدد المكررات لضمان دقة النتائج وتقليل التباين الإحصائي.
Questions related to the research
  1. ما هو الهدف الأساسي من هذه الدراسة؟

    الهدف الأساسي من هذه الدراسة هو الحصول على سلالات خضرية متحملة للملوحة وتقييم سلوكها للتأكد من توريث صفة تحمل الملوحة.

  2. ما هي الأصناف التي تم دراستها في هذه التجربة؟

    الأصناف التي تم دراستها هي Riviera وBurren.

  3. ما هي المعايير التي يمكن اعتمادها كمعيار انتخابي لتحمل الملوحة خارج الجسم الحي؟

    يمكن اعتماد ارتفاع النبات وعدد العقد/نبات كمعيار انتخابي لتحمل الملوحة خارج الجسم الحي.

  4. ما هي النتائج الرئيسية التي توصلت إليها الدراسة؟

    النتائج الرئيسية هي أن التشعيع كان فعالاً في تحفيز إخلاف النباتات من كالَس الصنف Riviera عند مستوى ملوحة 10 ديسي سيمنز/م، ومن كالَس الصنف Burren عند مستويات ملوحة 8 و12 ديسي سيمنز/م، وأن أقل نسبة انخفاض في ارتفاع النبات وعدد العقد وتكوين الدرينات كانت في السلالات الطافرة المتحملة للملوحة.


References used
Ahloowalia, B. S. (1997). In vitro selection of mutants. In: Somaclonal variation and mutagenesis in plant improvement and in vitro selection of mutants. 15th IAEA/FAO Interregional Training Course on Advances in Technologies for Induced Mutation in Crops. Siebersdorf, Vienna, Austria. 1-6
Al-Hatab, Z.; W. Quadhy; and D. Al-Ani (1992). Chromosomal changes of irradiated hexaploid whe�- at calli. Pp.465- 469. First Arab Conference on The Peaceful Uses of Atomic Energy, 23-rd Feb 1992, Tripoli, Libya
Azami, M.A.; M. Torabi; and F. Shekari (2010). Response of some tomato cultivars to sodium chloride stress under in vitro culture condition. Afr j Agric Res., 5(18):2589- 2592
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