Samples of Capparis spinosa plant were collected from 6 sites in Aleppo and Lattakia
provinces. The genetic variations were studied using the AFLP technique in order to determine the
genotypes of the studied types using 3 primers, which showed ampl
ification. Statistical analyses
were conducted using the dissimilarity coefficient and genetic diversity coefficient. The A.C.P. and
the statistical -F were calculated, and the similarity dendrogram was constructed. The results
showed the following:
The presence of a certain number of specific alleles (descriminates) for each province.
The presence of genetic and reproductive isolation deterrent to gene flow between the two
provinces.
The heterozygoty average was a little higher in Lattakia (0.486) than in Aleppo (0.481). The
mean of genetic diversity coefficient of primers and individuals was a little higher in Aleppo
(0.677) than in Lattakia (0.653). The population mean was very close: (0.759) in Aleppo and
(0.760) in Lattakia. This may be explained on the basis of silent and specific alleles due to deletion
mutations, different selective effects as well as the reproduction system in the two cities.
The greatest genetic distance in Aleppo was (0.381) noted between (Al Dahea and Al Shekh
saaed), and in Lattakia (0.38) noted between (Al Amroniah and Jabla). However, the greatest
genetic similarity in Aleppo was (0.637) noted between (Al Shekh Saaed and Turkman Bareh), and
in Lattakia (0.675) noted between (Wata Deirzenon and Jabla). The variation between these values
was graduated.
The study of statistical –F showed the effect of the similarity factor in some populations.
This was more significant in Aleppo than in Lattakia, which indicates that the reproduction system
is more closed in Aleppo, and this refers as well to inbreeding or self pollination which showed
high genetic variations in these populations when compared with Lattakia.
The results of this study helped in determining primers that can be used as molecular
markers in a breeding program for Capparis spinosa as a medicinal plant. This technique showed
high efficiency in studying the similarity relationships between these two cities.
The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorp
hic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
This research was conducted to detect the morphological and molecular
differences between golden henbane (Hyoscyamus aureus L) collected from
south Syria. Seeds were collected from six locations, then sterilized and grown
In Vitro. Grown plants we
re described for some morphological characters. The
molecular characterization was carried out using the method of Inter Simple
Sequence Repeats (ISSR) to detect genetic variations. The plants showed
significant (p<0.01) morphological differences among studied locations, Plant
height ranged from 14.97 to 18.97 cm and stem color ranged from red to green.
Trichome density also ranged between medium, high and very high.
Furthermore, significant differences in leaves dimensions were observed
according to the plant geographical location. The main root length ranged from
6.09 to 8.37 cm. Molecular analysis by ISSR produced a total of 56 bands, 49 of
which were polymorphic (87.5%), resulted from using 11 non-specific primers.
A genetic distance dendrogram was drawn. based on the results of genetic
characterization of plant from different locations. The plants collected from
Salkhad separated with a genetic distance of 0.312 while the plants collected
from other locations were grouped together, at the same time, they were
divided into two sub groups, the first group comprised plants collected from
Qalamoun and Dimas regions with a genetic distance of 0.032, whereas sub
group II content Bosra plants which separated clearly from those of Dara' dam
and Wadi Al-Zaidi by a genetic distance of 0.017, These results may indicate a
relationship between genetic characters of this plant species with geographic
distribution.
Shami goat in Syria is one of the important domestic breeds, because of its
high productivity and breeding capacity under the hard environmental
conditions and it is still being used in crossbreeding with Mountain goats as a
donor for milk produci
ng traits. This breed is still far away from scientific
investigations even it has been exposed to a random crossbreeding with
Mountain goats by goat breeders. This has lead to the possibility of losing some
of its genetic characteristics which are not detected yet. In this research the
genetic diversity among groups of pure Shami goats was determined using
ISSR-PCR on blood and results indicated that there was a molecular genetic
diversity among studied goats and polymorphic rate was register to 100%.
Random Amplified Polymorphic DNA (RAPD) was used to evaluate genetic
diversity among 11 Yemeny pomegranate types. Samples were collected in 2004
from three main regions: east, middle and north of the country (Rada, Hada
and Saada). PCR amplificati
on of pomogranate DNA with 30 random primers
generated 1013 bands, 118 of them were polymorphic 11.5%. Results showed
that the genetic diversity within the tested types was limited. Similarity values
ranged between 59 –96 % with a mean of 79 %. Statistical analysis showed
that the types can be divided into two clusters: cluster 1 with Orkobi and
Mellies types found in the Hada region; cluster 2 includes nine types belonging
to the Saada and Radaa regions .High similarity (90–96%) was obtained with
Khazemy, Liessy 1, Liessy 2, Taefi, Ahmer and Balady types. Whereas, some
types could be considerd as separate cultivars due to their high genetic
diversity such as, Orkoby and Mellies (Hada region) in addition to Bohssom,
Sommaty and Liessy 3 in the Saada region.
يعد الماعز الشامي في سورية من الحيوانات الزراعية المحلية الهامة لما يملكه من مزايا ومواصفات تمكنه من الإنتاج والتناسل تحت الظروف البيئية القاسية لهذا فقد استخدم في برامج الخلط التربوي مع الماعز الجبلي كمعطي لمورثات صفة إنتاج الحليب الجيدة للحيوانات الخليطة
The study was conducted on the type of peach, Prunus cerasia, which is widespread in the wild in Lattakia , at the Scientific Agricultural Research Center in Lattakia during the years (2012-2014) AD.
