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Register a new userStudy the effects of some plant growth regulators on multiplication and rooting in vitro of (Ceartonia siliqua L.)
دراسة تأثير بعض منظمات النمو في مرحلتي الإكثار و التجذير لنبات الخرنوب .Ceratonia siliqua L في الزجاج
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Damascus University ورقة بحثية
Publication date
2013
fields
Biology
and research's language is
العربية
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Shamra Editor
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أجري هذا البحث على نبات الخرنوب .Ceratonia siliquaL المكاثر مخبرياً بهدف دراسة تأثير بعض منظمات النمو في مرحلتي الإكثار و التجذير، و من ثم تحديد التراكيز الفُضلى منها التي تؤدي إلى أعلى معدل إكثار (من حيث عدد النموات المتشكلة و طولها)، و أفضل تجذير (من حيث نسبة التجذير و عدد الجذور و طولها).
This research was conducated on Ceratonia siliqua L. in vitro to study the effect of some growth regulators in both multiplication and rooting stage. And determine the best concentrations wich lead the highest rate of multiplication (interms of number of shoots and length), and the best rooting (interms of percentage of rooting, number of roots and length).
References used
Allen, O. N. and Allen, E. K. (1981). The leguminoceae. The university of Wisconsin press Madison, W1.812 pages
(Battle, I and Tous, J. (1998). Carob tree (Ceratonia siliqua L.) promoting the conservation and use of under-utilised and neglected crops. Roma: Institute of plant genetics and crop plant research; Gatersleben: International plant genetic resource institute(IPGRI
Brugaletta, M.; Lamalfa, S.; Gentile, A.; Almeida, R. and Romano, A. (2007). In vitro culture establishment of ceratonia siliqua(L) mature trees from cultivars of different Mediterranean countries. ISHS Actahorticulturae 812:lll international symposium on a cclimatization and establishment of micropropagated plants
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Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus
communis L.) grown in the field under natural conditions at Damascus
countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected
by 70% Ethanol an
d Clorox containing 5.25 % Sodium Hypoclorite
with a drop of Tween 20 for different periods and concentrations before being
placed onto MS basal medium. Established cultures were then transferred onto
MS basal medium containing a combination of growth regulators at different
concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM
or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of
12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44
μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
The effect of different treatments on carob seed germination was studied.
The results showed that, seed scarification was the most effective method in
increasing germination rate as well as the use of concentrated sulfuric acid for 30 minutes
reac
hing 100%, followed by the treatment with alcohol for 60 minutes, and immersing
seeds in hot water for 60 minutes.
The results also indicated that the speed of germination when seeds were scarified
was the best (I.D.=14.5), followed by the treatment with concentrated sulfuric acid for 30
minutes (I.D.=21.5), alcohol for 60 minutes (I.D.= 38) and immersing seeds in hot water
for 60 minutes (ID.=60).
This study aimed to obtain seedlings balanced and superior in growth, by searching
the effect of seed size on the growth and development of seedlings of carob tree
( Ceratonia siliqua L.) developing in containers Minirhizotron in the growth room in
Research Center in Bouka-Lattakia. Seeds were divided into three categories (large-size
LS, control CS, small-sized SS) and studied several quantities indicators of growth and
development of the total shoot and root of the seedlings for a period of three months.
For a radical system, results indicated a high significant superiority in the large
seeds values for the main root of the overall growth rate such as length (cm) and the speed
of growth (cm/3days) and secondary roots (total number, total length/cm) height to control
and small seeds. And the significant superiority of the seeds of the control on the small
seeds.
It has also given large seeds higher values regarding all indicators related to the shoot
system such as average diameter and average height and average number of phalanges
stem and leaves, and ending with the dry weight of shoots.
Results for the study of periodic growth curves for the main root and main stem of
the three treatments indicated the presence of a great similarity in the overall appearance of
these curves, which refers that the appearance is not affected by this change in the amount
of seed size. On the other hand, the phenomenon was observed in contrast growth
Antagonism clearly between the totals shoot and root with any end to the growth of main
root accompanied by a junior at the end of the main stem growth and vice versa.
Ziziphora canescens is a species of important medicinal plants in Syria due
to its medicinal properties as antibiotic, flavors and spices in various foods.
This plant is important, especially in folk medicine in some areas (Kalamoon)
on the one ha
nd, and retreat of its spread which may lead eventually to its
extinction on the other hand, so a protocol for rapid micropropagation has
been developing by using lateral and apical buds on nutrient media MS
supplemented with different types and concentrations of plant growth
regulators.
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
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