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Study the effects of some plant growth regulators on multiplication and rooting in vitro of (Ceartonia siliqua L.)

دراسة تأثير بعض منظمات النمو في مرحلتي الإكثار و التجذير لنبات الخرنوب .Ceratonia siliqua L في الزجاج

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 Publication date 2013
  fields Biology
and research's language is العربية
 Created by Shamra Editor




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This research was conducated on Ceratonia siliqua L. in vitro to study the effect of some growth regulators in both multiplication and rooting stage. And determine the best concentrations wich lead the highest rate of multiplication (interms of number of shoots and length), and the best rooting (interms of percentage of rooting, number of roots and length).


Artificial intelligence review:
Research summary
أجريت هذه الدراسة على نبات الخرنوب (Ceratonia siliqua L.) في المختبر لدراسة تأثير بعض منظمات النمو في مرحلتي الإكثار والتجذير. هدفت الدراسة إلى تحديد التراكيز المثلى لمنظمات النمو التي تؤدي إلى أعلى معدل إكثار (من حيث عدد النموات المتشكلة وطولها) وأفضل تجذير (من حيث نسبة التجذير وعدد الجذور وطولها). أظهرت النتائج أن أفضل تركيبة للإكثار كانت باستخدام 3.11µM BAP و1µM IBA، حيث بلغ متوسط عدد النموات 3.3 وطولها 3.4 سم. أما في مرحلة التجذير، فكانت أفضل النتائج باستخدام 4.9µM IBA، حيث بلغت نسبة التجذير 90% ومتوسط طول الجذور 2.7 سم. كما بلغت نسبة نجاح عملية الأقلمة 95%.
Critical review
دراسة نقدية: الدراسة قدمت نتائج قيمة حول تأثير منظمات النمو على إكثار وتجذير نبات الخرنوب في المختبر، مما يسهم في الحفاظ على هذا النبات المهم. ومع ذلك، كان يمكن تحسين الدراسة من خلال تضمين مزيد من التفاصيل حول الظروف البيئية المحددة التي تم فيها إجراء التجارب، وكذلك من خلال إجراء تجارب ميدانية للتحقق من نتائج المختبر. بالإضافة إلى ذلك، كان يمكن توسيع نطاق الدراسة ليشمل تأثير منظمات النمو الأخرى أو تراكيز مختلفة للحصول على صورة أكثر شمولية. بشكل عام، الدراسة تعد خطوة إيجابية نحو تحسين تقنيات زراعة الخرنوب، ولكن هناك مجال للتحسين والتوسع في الأبحاث المستقبلية.
Questions related to the research
  1. ما هي أفضل تركيبة لمنظمات النمو لتحقيق أعلى معدل إكثار لنبات الخرنوب؟

    أفضل تركيبة لمنظمات النمو لتحقيق أعلى معدل إكثار كانت باستخدام 3.11µM BAP و1µM IBA، حيث بلغ متوسط عدد النموات 3.3 وطولها 3.4 سم.

  2. ما هي نسبة نجاح عملية التجذير باستخدام 4.9µM IBA؟

    نسبة نجاح عملية التجذير باستخدام 4.9µM IBA بلغت 90%، مع متوسط طول الجذور 2.7 سم.

  3. ما هي نسبة نجاح عملية الأقلمة للنباتات بعد التجذير؟

    نسبة نجاح عملية الأقلمة للنباتات بعد التجذير بلغت 95%.

  4. ما هي أهمية دراسة تأثير منظمات النمو على نبات الخرنوب؟

    أهمية دراسة تأثير منظمات النمو على نبات الخرنوب تكمن في تحسين تقنيات إكثاره وتجذيره في المختبر، مما يسهم في الحفاظ على هذا النبات المهم وزيادة إنتاجيته.


References used
Allen, O. N. and Allen, E. K. (1981). The leguminoceae. The university of Wisconsin press Madison, W1.812 pages
(Battle, I and Tous, J. (1998). Carob tree (Ceratonia siliqua L.) promoting the conservation and use of under-utilised and neglected crops. Roma: Institute of plant genetics and crop plant research; Gatersleben: International plant genetic resource institute(IPGRI
Brugaletta, M.; Lamalfa, S.; Gentile, A.; Almeida, R. and Romano, A. (2007). In vitro culture establishment of ceratonia siliqua(L) mature trees from cultivars of different Mediterranean countries. ISHS Actahorticulturae 812:lll international symposium on a cclimatization and establishment of micropropagated plants
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This study aimed to obtain seedlings balanced and superior in growth, by searching the effect of seed size on the growth and development of seedlings of carob tree ( Ceratonia siliqua L.) developing in containers Minirhizotron in the growth room in Research Center in Bouka-Lattakia. Seeds were divided into three categories (large-size LS, control CS, small-sized SS) and studied several quantities indicators of growth and development of the total shoot and root of the seedlings for a period of three months. For a radical system, results indicated a high significant superiority in the large seeds values for the main root of the overall growth rate such as length (cm) and the speed of growth (cm/3days) and secondary roots (total number, total length/cm) height to control and small seeds. And the significant superiority of the seeds of the control on the small seeds. It has also given large seeds higher values regarding all indicators related to the shoot system such as average diameter and average height and average number of phalanges stem and leaves, and ending with the dry weight of shoots. Results for the study of periodic growth curves for the main root and main stem of the three treatments indicated the presence of a great similarity in the overall appearance of these curves, which refers that the appearance is not affected by this change in the amount of seed size. On the other hand, the phenomenon was observed in contrast growth Antagonism clearly between the totals shoot and root with any end to the growth of main root accompanied by a junior at the end of the main stem growth and vice versa.
Ziziphora canescens is a species of important medicinal plants in Syria due to its medicinal properties as antibiotic, flavors and spices in various foods. This plant is important, especially in folk medicine in some areas (Kalamoon) on the one ha nd, and retreat of its spread which may lead eventually to its extinction on the other hand, so a protocol for rapid micropropagation has been developing by using lateral and apical buds on nutrient media MS supplemented with different types and concentrations of plant growth regulators.
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