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Ten medicinal plants were chosen from various plants in Syria which used as spices or cooked and Syria traditional medicine. Their names Rosemary, Damask rose, Chamomile, Sumac, Silver linden, Black cumin, Thyme, Mint, Laurel and lemon balm. Mois ture, yield of extraction, total phenolic content (TPC) and antioxidant activities of methanol, ethanol, acetone and aqueous extracts of medicinal plants were investigated. The TPC were measured by Folin-Ciocalteu method, and the antioxidant activity was assessed by the scavenging activity towards 2, 2′-diphenyl- 1-picryl hydrazyl (DPPH)-radical.
Classified locality Yeasts have been culturing in plantology department at the Faculty of Science-University of Damascus, from genera of Pichia, Candida, Geotrichum, Rhodotorula, Phaffia on solid Czhapek agar medium, and after incubated in appropr iate conditions their colonies showed evidence of different colors to produce a variety of pigments. Then it cultured in liquid Czhapek medium to increase biomass and the genera producing endocellular pigment separated from the medium by filtration with added ethanol on them and blasting cells by ultrasonic waves to get a pigment, while the genera producing exocellular pigment is separated about the liquid medium also by filtration but the solvent was added to the medium containing the pigment.
Functional foods are similar to conventional food and demonstrate beneficial effects beyond their nutritional value. These effects are attributed to the presence of functional ingredients. Functional ingredients can either be found naturally in a c ertain food or added to it. There’s a number of functional food products available in the market. For example, natural functional juices containing polyphenols. The aim of this study is to determine total phenolic content and antioxidant activity in some local functional juices. Total phenolic content was determined according to Folin- Ciocalteu method. The highest in content was mulberry juice, while the lowest was red grape juice. The best conditions for eliminating ascorbic acid were also determined which were heating for 30 min at 75°C. Antioxidant activity was subsequently determined using potassium ferricyanide. The highest in phenolic content among the studied juices were the highest in antioxidant activity. Thus a strong correlation was found between total phenolic content and antioxidant activity.
Antimicrobial compounds have been used for decades in poultry diets to increase performance and decrease morbidity particularly in broiler chickens. However, consumer pressure related to the potential development of antibiotic-resistant bacteria ha s resulted in the development of non-antibiotic feed additives that may also improve broiler erformance. In recent years, aromatic plants and their extracts have received attention as growth and health promoters. Such products have several advantages over commonly used commercial antibiotics since they are residue free and they are also, generally recognized as safe and commonly used items in the food industry. The antimicrobial properties of medical plants and their extracts have been widely reported because they consist of many active ompounds such as carvacrol, eugenol,which partition lipids in the bacterial cell wall and mitochondria, disturbing the structures and rendering them more permeable. Generally,medical plants are slightly more active against Gram-positive than Gramnegative bacteria. Medical plants are well known for their antioxidant activity and show a considerable effect on increasing the antioxidant capacity of chicken meat because they consist of many of active compounds such as tocopherol, carvacrol. Spices or their active principles have been reported to stimulate digestive secretions such as lipase, amylase and proteases.because they consist of many active compounds such as anothol, eugenol. Variable effects of medical plants and their extracts on the productive performance of broiler have been reported. The majority of experimental results indicate improved body weight gain or final body weight and feed conversion ratio.
Additive migration levels in food simulants from polymeric materials that are intended to be into contact with food can be affected by additive stability under the migration test conditions. In this work, the stability of four antioxidants: Butyle ted hydroxy toluene (BHT), Bisphenol A (BPA), Pentaerythritol tetrakis (3,5-di-tert-butyl-4-hydroxyhydrocinnamate) (Irganox 1010), and Octadecyl 3-(3,5-di-tert-butyl-4-hydroxyphenyl) propionate (Irganox 1076), was studied in aqueous food simulants A, B, C and ethanol 95% (as a substitute for olive oil- which is a fatty food simulant- simulant D), at different temperatures 20, 40 and 70 ºC, during 20 days. Samples were analyzed by high performance liquid chromatography (HPLC) with UV detector. In general, the studied compounds appeared to be more stable in both Ethanol (95%) and simulant B than in simulants A and C.
The aim of this research, is to use Liquid–liquid extraction to separate phenolic compounds from olive mill wastewater resulted from three-phase centerfuge extraction (OMWW). Four solvents were used: Methyl iso butyl ketone (MIBK), Ethyl acetate ( EtOAc), Octanol-1 (C8H18O), and Noctanoylpyrrolidine (OPOD) which was synthesized in our lab. The effects of pH and Extraction stages on the efficiency of total phenolic compound extracted from OMWW were determined by Folin - Ciocalteu spectrophotometric method. Ferulic acid (FA) and Caffeic acid (CA) were determined in extractants by HPLC. The COD value in water phase of the four extracts was measured together with the reducing power and radicalscavenging effects for the extracts. We compared the antioxidant activity of MIBK, OPOD extracts, with ferulic acid (FA), caffeic acid (CA), ascorbic acid standards and rosemary. The results showed that MIBK had the highest extraction efficiency and gave the maximum quantity of phenols from OMWW, than OPOD, C8H18O, EtOAc. The COD value was decreased about %94.5 by MIBK, %83.7 by OPOD,%81 by C8H18O, and%72 by EtOAc. The phenols extracted by MIBK could be added to eatable oils as natural antioxidants, because they were superiority on rosemary, and nearly to caffeic acid (CA) standard.
Four varieties of grape raisins where made in traditional way and investigated for their chemical composition, mineral elements and total antioxidanst capacity. Results reveals that there were significant differences in the average percentage of m oisture and carbohydrate between varieties, whereas the protein, fat and ashes percentages were not significant. Results of minerals analysis showed that blue raisins had the highest amount of Calcium, phosphorous and potassium with a level of 66 mg, 193 mg and 989 mg in 100 g raisins respectively. In addition, the total polyphenol contents by Folin- Ciocalteau assay were higher in blue (257 mg) and red raisins (213mg) when compared to the others varieties and vitamin C was 45mg/100g and 34 mg/100g in blue and red raisins respectively. The antioxidant activity of raisins was assessed by the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay. The amount of raisins needed to scavenge 50% of DPPH radical (EC50) was similar for all varieties ranging from about 4.18 mg to 6.41 mg. Total antioxidants capacity were in descending order blue >red > white > green raisins. Therefore, the consumption of raisins is considered to contribute the intake of antioxidants and minerals in Syrian diet .
Some local natural sources of antioxidant such as orange peel extract (Citrus sinensis), pomegranate peel extract (Punica granatum) and ginger extract (Zingiber officinale L.) were evaluated during 5 days storage of refined palm oil at 63°C as com pared to the synthetic antioxidant such as propyl gallate. The above extracts were obtained with ethanol and oil (palm oil). Those extracts were added to the oil at two different levels (100 ppm and 200 ppm). Antioxidant activity of natural extracts, synthetic antioxidant and the control treatment without antioxidant under thermal treatment (Schaal test) was assessed by measuring acid value (AV), peroxide value (PV), iodine value (IV) and refractive index (RI) during 6 days storage of refined palm oil at temperature mentioned above. Antioxidant activity also measured by studying the stability of oil by the use of rancimat method. All treatments exhibited antioxidant activity. Statistical analysis results were shown that the pomegranate peel extract treatment was significantly (P<0.05) superior than the orange peel extract and ginger extract treatments whereas, it was not significant difference about propyl gallate treatment.
The Bcl-2 family of proteins has been implicated as a necessary factor in the death caused by a large number of different agents of a wide variety of cell types. Cystine is a precursor for the biosynthesis of the intracellular Thiol, (glutathione) GSH. The mechanism by which Bcl-2 resist cystine deprivation induced apoptosis was investigated in hepatocyte Hepz/Bcl-2 and Hepz/pEF cell lines.
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