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Register a new userDetection the Antioxidant Activity of some local yeasts extracts
تحديد النشاط المضاد للأكسدة لمستخلصات بعض الخمائر المحلية
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Aِl-Baath University ورقة بحثية
Publication date
2015
fields
Biology
and research's language is
العربية
Created by
Shamra Editor
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تم استزراع خمائر محلية سبق و ان صنفت في قسم علم الحياة النباتية بكلية العلوم-جامعة دمشق، من أجناس Pichia, Candida, Geotrichum, Rhodotorula, Phaffia على وسط شابك الصلب Czhapek agar, و بعد حضنها في الشروط الملائمة أظهرت مستعمراتها ألواناً مختلفة دليلا على إنتاجها لأصبغة متنوعة. ثم استزرعت على وسط شابك السائل لزيادة كتلتها الحيوية و بعدها فصلت الأجناس المنتجة للصباغ بشكل داخل خلوي عن الوسط بالترشيح و أضيف لها الإيتانول و فُجِّرت الخلايا بوساطة الأمواج فوق الصوتية للحصول على الصباغ، أما الأجناس المنتجة للصباغ بشكل خارج خلوي فصلت عن الوسط السائل بالترشيح أيضاً، لكن المذيب أضيف للوسط الحاوي على الصباغ.
Classified locality Yeasts have been culturing in plantology department at the Faculty of Science-University of Damascus, from genera of Pichia, Candida, Geotrichum, Rhodotorula, Phaffia on solid Czhapek agar medium, and after incubated in appropriate conditions their colonies showed evidence of different colors to produce a variety of pigments. Then it cultured in liquid Czhapek medium to increase biomass and the genera producing endocellular pigment separated from the medium by filtration with added ethanol on them and blasting cells by ultrasonic waves to get a pigment, while the genera producing exocellular pigment is separated about the liquid medium also by filtration but the solvent was added to the medium containing the pigment.
References used
Ashok BT, Ali R, 1999- The aging paradox: free radical theory of aging. Experimental GerontologyVolume 34, Issue 3, June 1999, Pages 293–303 Mini-Reviews
Banzatto D, Lidyane A, Rossini M, Marcia J, 2013- Carotenoid production by Rhodotorula rubra cultivated in sugarcane juice, molasses, and syrup. Source: Food Science and Technology, Volume: 33, Pages: 14-18
Dufossé L, 2006- Microbial Production of Food Grade Pigments. Food Technol Biotechnol, 44 (3) 313–321
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