Classified locality Yeasts have been culturing in plantology
department at the Faculty of Science-University of Damascus,
from genera of Pichia, Candida, Geotrichum, Rhodotorula, Phaffia
on solid Czhapek agar medium, and after incubated in appropr
iate
conditions their colonies showed evidence of different colors to
produce a variety of pigments. Then it cultured in liquid Czhapek
medium to increase biomass and the genera producing
endocellular pigment separated from the medium by filtration with
added ethanol on them and blasting cells by ultrasonic waves to
get a pigment, while the genera producing exocellular pigment is
separated about the liquid medium also by filtration but the solvent
was added to the medium containing the pigment.
Antimicrobial compounds have been used for decades in poultry diets to increase
performance and decrease morbidity particularly in broiler chickens. However, consumer
pressure related to the potential development of antibiotic-resistant bacteria ha
s resulted in
the development of non-antibiotic feed additives that may also improve broiler erformance.
In recent years, aromatic plants and their extracts have received attention as growth and
health promoters. Such products have several advantages over commonly used commercial
antibiotics since they are residue free and they are also, generally recognized as safe and
commonly used items in the food industry. The antimicrobial properties of medical plants
and their extracts have been widely reported because they consist of many active
ompounds such as carvacrol, eugenol,which partition lipids in the bacterial cell wall and
mitochondria, disturbing the structures and rendering them more permeable.
Generally,medical plants are slightly more active against Gram-positive than Gramnegative
bacteria. Medical plants are well known for their antioxidant activity and show a
considerable effect on increasing the antioxidant capacity of chicken meat because they
consist of many of active compounds such as tocopherol, carvacrol. Spices or their active
principles have been reported to stimulate digestive secretions such as lipase, amylase and
proteases.because they consist of many active compounds such as anothol, eugenol.
Variable effects of medical plants and their extracts on the productive performance of
broiler have been reported. The majority of experimental results indicate improved body
weight gain or final body weight and feed conversion ratio.
The aim of this research, is to use Liquid–liquid extraction to separate
phenolic compounds from olive mill wastewater resulted from three-phase
centerfuge extraction (OMWW). Four solvents were used: Methyl iso butyl
ketone (MIBK), Ethyl acetate (
EtOAc), Octanol-1 (C8H18O), and Noctanoylpyrrolidine
(OPOD) which was synthesized in our lab. The effects of
pH and Extraction stages on the efficiency of total phenolic compound
extracted from OMWW were determined by Folin - Ciocalteu
spectrophotometric method. Ferulic acid (FA) and Caffeic acid (CA) were
determined in extractants by HPLC. The COD value in water phase of the four
extracts was measured together with the reducing power and radicalscavenging
effects for the extracts. We compared the antioxidant activity of
MIBK, OPOD extracts, with ferulic acid (FA), caffeic acid (CA), ascorbic acid
standards and rosemary.
The results showed that MIBK had the highest extraction efficiency and
gave the maximum quantity of phenols from OMWW, than OPOD, C8H18O,
EtOAc. The COD value was decreased about %94.5 by MIBK, %83.7 by
OPOD,%81 by C8H18O, and%72 by EtOAc. The phenols extracted by MIBK
could be added to eatable oils as natural antioxidants, because they were
superiority on rosemary, and nearly to caffeic acid (CA) standard.
