Background& Objective: Onychomycosis is a frequent nail disease caused by dermatophytes, yeasts, and
non-dermatophyte molds. Far more than being a simple cosmetic problem, infected nail serves as a
chronic reservoir, which can give rise to repeated
mycotic infections.
This study aim to identify the common fungal species which are responsible for onychomycosis in
clinically suspected patients of onychomycosis in Dermatology and Venereal Diseases Hospital in
Damascus between October 2010 and July 2011.
Materials & Methods: Both direct microscopy and culture of the nail material were performed to identify
the causative fungi of onychomycosis.
Results: Culture positivity was obtained in 65 of the 76 clinically suspected samples, etiological fungal
agents were (%61.5) dermatophytes, (24.6%) yeasts, and (13.9 %) non-dermatophyte molds . The isolated
fungal species were (43.1%) Trichophyton Rubrum and (16.9%) T. Interdigitale (1.5%) T.Verrucosum
Aspergillus Versicolor which was the most frequent non dermatophyte molds. Females were affected in
fingernails more frequently than males, while males were affected in toenails more frequently than
females, and in both sexes those most infected were between 20-40 years of age.
Conclusion: Dermatophytes, in particular T. rubrum, but also T. Interdigitale, are the most frequently
isolated causative agents in onychomycosis in patients seen in Dermatology and Venereal Diseases
Hospital in Damascus . In addition, yeasts may be isolated relatively frequently, while molds are
uncommon.
Classified locality Yeasts have been culturing in plantology
department at the Faculty of Science-University of Damascus,
from genera of Pichia, Candida, Geotrichum, Rhodotorula, Phaffia
on solid Czhapek agar medium, and after incubated in appropr
iate
conditions their colonies showed evidence of different colors to
produce a variety of pigments. Then it cultured in liquid Czhapek
medium to increase biomass and the genera producing
endocellular pigment separated from the medium by filtration with
added ethanol on them and blasting cells by ultrasonic waves to
get a pigment, while the genera producing exocellular pigment is
separated about the liquid medium also by filtration but the solvent
was added to the medium containing the pigment.
Rhodotorula yeast was isolated from various local sources. Fifty isolates
were collected during the years 2012-2013 where 13 were isolated from soil, 23
from tree leaves and 14 from food. The isolates AUX system. Were classified
into three Rhodoto
rula species: R. mucilaginosa, R. glutinis and R. minuta with
76%, 20% and 4% respectively using API 20c. Six methods were applied in
breaking down the yeast cells and extracting the carotenoids. It was shown the
quantity of carotenoides extracted with the modified method by adding DMSO
to the yeast biomass and incubating for 24 hrs at 4˚C yielded in higher
quantities when compared with the other five methods. All isolates were able to
produce carotenoides but they varied in their efficiency where the isolate A24
(R.mucilaginosa) isolated from food was distinguished by its high level of
production which reached 658.23 μg/g dry weight compared with the others.
Thirty randomized samples of sugar materials were collected from different
places (Tartous, Damascus, Homs) in Syria for isolation and identification of
some yeasts. These yeasts were classified physiologically by API technique
to identify the spe
cies of Yeasts found in these materials. Results showed that
the isolated materials contained 17 isolated yeasts distributed in 3 genus and 7
species and named as follows: One isolated yeast from Geotrichum capitatum,
Candida krusei,Candida guilliermondi, Candida fumata and Candida zeylanoides
and 4 isolated yeasts from Candida lusitaniae and 7 isolated yeasts from
Saccharomyces cerevisiae. The classified yeasts were screened to investigate the
activity of internal and external invertase produced by them using
spectrophotometer at 540 nm. The results showed that there were differences in
the ability of yeasts to produce invertase enzyme. Candida Lusitaniae-2 and
Saccharomyces cerevisiae-4 were found the best yeasts for production of
external invertase, whereas Candida Lusitaniae1 was the best for internal
invertase.
Sixty yeasts spp. were isolated from samples of whey which collected from
different locations in Syria. These yeasts were classified morphologically
according to Barnett’s and physiologically by API technique to identify the
species of Yeasts, whe
re had found 10 classified yeasts as follows:
Candida krusei is the most of yeasts which founded in whey with 21.66 %,
and in the Second rank is Candida lusitaniae with 16.66 %. the third rank came
(Geotrichum capitatum, Saccharomyces cerevisiae, Rhodotorula minuta) with
11.66%, after that the Candida magnolia is ranked in the fourth rank by
distributing with 8.33%. as soon as the Candida famata came in the fifth rank
with 6.66%, and in the sixth rank we founded Candida lipolytica with 5%,
finally the Rhodotorula. mucilaginosa and Candida. kefyr came in the last rank
with 3.33%, so we noticed the variety of the yeasts in the studied locations in
Syria.
Since the beginning of the twentieth century, researchers have isolated and
dentified spreaded and adapted yeasts to variow environmental conditions and
the types of the mediums in which they graw. The industrial features of the
isolated genera, e
specially those used in the food processing and the stability of
their genetic characteristics were studied. Yeasts in medical and industrial
fields were also improved.
The objective of this research was to study the causes , symptoms
and diagnosis of otitis externa in cats, and to investigation the effect
, breed, season on the rate of affection in the veteriniary faculity in
hama university.
The number of case
s was( 346) wich was broght to the
veterinary teaching hosbital in Hama university and from special
clinics.
