The Mediterranean region is world’s leading olive growing area. A large amount of
liquid waste results from olive oil extraction these wastewaters have a different effects an
environmental . In this study, four fungal isolates from Samples of soil
Polluted by olive
mill wastewater ,this isolates follow to several types from deuteromycetes, they are follow
to this types : Humicola sp., paecilomyces javanicus, Aspergillus terreus, Aspergillus
flavus. They isolated them on tow media (PDA, CzA) and identify them on colony
character ,Biometric and compare them with reference studies.
The aim of this research, is to use Liquid–liquid extraction to separate
phenolic compounds from olive mill wastewater resulted from three-phase
centerfuge extraction (OMWW). Four solvents were used: Methyl iso butyl
ketone (MIBK), Ethyl acetate (
EtOAc), Octanol-1 (C8H18O), and Noctanoylpyrrolidine
(OPOD) which was synthesized in our lab. The effects of
pH and Extraction stages on the efficiency of total phenolic compound
extracted from OMWW were determined by Folin - Ciocalteu
spectrophotometric method. Ferulic acid (FA) and Caffeic acid (CA) were
determined in extractants by HPLC. The COD value in water phase of the four
extracts was measured together with the reducing power and radicalscavenging
effects for the extracts. We compared the antioxidant activity of
MIBK, OPOD extracts, with ferulic acid (FA), caffeic acid (CA), ascorbic acid
standards and rosemary.
The results showed that MIBK had the highest extraction efficiency and
gave the maximum quantity of phenols from OMWW, than OPOD, C8H18O,
EtOAc. The COD value was decreased about %94.5 by MIBK, %83.7 by
OPOD,%81 by C8H18O, and%72 by EtOAc. The phenols extracted by MIBK
could be added to eatable oils as natural antioxidants, because they were
superiority on rosemary, and nearly to caffeic acid (CA) standard.
The Bio-mass was isolated from the medium of the water olive
soaking , and was developed on two medium olive mill wastewater
OMW.
