The aim of this work was determining the role of
Protein kinase C (PKC) signaling pathway in apoptosis of
Leishmania tropica promastigotes, through studying effects of PKC
specific inhibitor Staurosporine (STS) on the parasite. Our results
showed
that treated L. tropica with Staurosporine was
enough to inhibit PKC pathway completely, while viability test
showed that the concentration of STS to inhibit cell growth by 50%
(IC50 = 2.146μM).
P53 is the guardian of genome from any alteration, its gene is called tumor suppressor gene . p53 checks
any alteration in cell cycle ,and if the alteration happened p53 resolves or shares in activation proteins
important in resolving , if the cell
couldn't resolve the alteration p53 leads it to apoptosis . goingthe cell
out of this nature cycle and continuing its resistance and proliferation makes it a tumor celland that make
p53 very important study.
The Bcl-2 family of proteins has been implicated as a necessary factor in the
death caused by a large number of different agents of a wide variety of cell
types. Cystine is a precursor for the biosynthesis of the intracellular Thiol,
(glutathione)
GSH.
The mechanism by which Bcl-2 resist cystine deprivation induced apoptosis
was investigated in hepatocyte Hepz/Bcl-2 and Hepz/pEF cell lines.
