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Register a new userPhenols extraction from olive mill wastewater using different solvents and studying the antioxidant activity of the extractants
استخلاص الفينولات من مياه عصير الزيتون بمحلات مختلفة و دراسة فعالية المستَخلصات كمضادات أكسدة
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هدفَ هذا البحث إلى استخدام تقانة الاستخلاص سائل – سائل لفصل المركبات الفينوليـة المرافقـة للمياه الناتجة مباشرة من فرازة عصر ثمرة الزيتون بطريقة الطرد المركزي ثلاثي الطور (OMWW). اِستُخدمت أربعة أنواع مختلفة من المحلاّت العضوية و هي خلات الإيتيل (EtOAc) و ميتيل إيزو بوتيـل كيتون (MIBK) و الأوكتانول-1) C8H18O (وN -أوكتانويل بيروليدين (OPOD) و الأخيـر اِصـطُنع مخبرياً. درس تأثير تغير pH الوسط و عدد مرات الاستخلاص في الكمية الكلية للفينولات المستَخلصة من OMWW و ذلك باستخدام طريقة فولين- سيوكالتيو. حددت كمية كل من حمض الكافئيك CA و حمض الفيروليك FA الموجودة في المستَخلصات باستخدام تقانة الـ HPLC .درِسـت فعاليـة الاسـتخلاص بالمحلات الأربعة على تخفيض الملوثات العضوية الناتجة عن مياه عصير الزيتون بطريقة تحديـد الــ COD ،درست فعالية المستخلصات كمضادات أكسدة عن طريق تعيين قدرتها الإرجاعية و قدرتها علـى كبح الجذور الحرة، كما استخدمت طريقة الأكسجين الفعال للمقارنة بين فعاليـة مستخلـصي MIBK و OPOD كمضادات أكسدة و مقارنتهما بحمضي الكافئيك و الفيروليك العياريين و مضاد الأكـسدة الطبيعـي إكليل الجبل (Rosemary) المسوق تجارياً.
The aim of this research, is to use Liquid–liquid extraction to separate phenolic compounds from olive mill wastewater resulted from three-phase centerfuge extraction (OMWW). Four solvents were used: Methyl iso butyl ketone (MIBK), Ethyl acetate (EtOAc), Octanol-1 (C8H18O), and Noctanoylpyrrolidine (OPOD) which was synthesized in our lab. The effects of pH and Extraction stages on the efficiency of total phenolic compound extracted from OMWW were determined by Folin - Ciocalteu spectrophotometric method. Ferulic acid (FA) and Caffeic acid (CA) were determined in extractants by HPLC. The COD value in water phase of the four extracts was measured together with the reducing power and radicalscavenging effects for the extracts. We compared the antioxidant activity of MIBK, OPOD extracts, with ferulic acid (FA), caffeic acid (CA), ascorbic acid standards and rosemary. The results showed that MIBK had the highest extraction efficiency and gave the maximum quantity of phenols from OMWW, than OPOD, C8H18O, EtOAc. The COD value was decreased about %94.5 by MIBK, %83.7 by OPOD,%81 by C8H18O, and%72 by EtOAc. The phenols extracted by MIBK could be added to eatable oils as natural antioxidants, because they were superiority on rosemary, and nearly to caffeic acid (CA) standard.
References used
Vasundhara, S., Vijay, K. H. & Jagan. M. R. J. 2008. Influence of milk and sugar on antioxidant potential of black tea. Food Research International. 41(2) 124-129
Barlow, S. M. 1990. Toxicological aspects of antioxidants used as food additives. In Hudson. Food antioxidants. 253-307
Yang, L., Han, H., Nakamura, N., Hattori, M. Wang, Z. Bio-guided. 2007. Isolation of antioxidants from the stems of Dendrobium aurantiacum var. Phytotherapy Research. 21(7).696-698
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The phenolic compounds were extracted from leaves and flowers of Hops and grape
seeds from local production by soaking with different solvents (Methanol-ethanol-alcohol
and water mixture) . The polyphenols content in the methanol extracts for 100 g
dry matter
was 6.31 g for the grape seeds, 2.08 g for Hops leaves and 0.6 g for Hops flowers.
Methanol extracts were characterized by an antioxidant activity that appeared by their
ability to Reduce ions ferriques Fe+3, at a concentration of 2500 ppm, the Reducing Power
was 96.45% , 67.56% for the grape seeds and Hops leaves, respectively. The methanolic
extracts of grape seeds and Hops leaves at 80 ppm showed activity in scavenge free radical
DPPH. was 82.04% and 40.92% respectively. The methanolic extracts of grape seeds and
Hops leaves at 250 ppm gave the ability to protect linoleic acid from the oxidation process
of 78.98% and 48.41%, respectively. The antioxidant activity values of the extracts were
compared with standard samples of ascorbic acid and BHT.
A field experiment was conducted in Tartous Governorate, on
the Arachis hypogaea . L during tow growing season (2012-
2013)(2013-2014). Experiment was included 6 treatments with
three replicates: control (without fertilization), different rates of
Olive Mail Waste Water (OMWW) (5-10-15) l/m2 , organic
fertilizer (30 tn/h)and nitrogen fertilizer(30)kg/h.
The research aimed to know effect of these Additions on some
physiological groups of microorganisms, nodulation on plant
roots and nitrogen relative bacteria Arachis hypogaea . L. yield.
The antioxidant activity of pomegranate juices extracted by two methods
was determined using three methods: β- carotene, Linoleic acid emulsion, and
DiPhenel Picryl Hydrazyl (DPPH). The juice was extracted by separation arils
from fruits and squee
zed using Cyclone machine (T-1), while the second
extraction method included squeezing the whole halve fruit with previous
machine, (T-2). The potential compounds were determined using ethanol in a
comparison with Butylated hydroxyl Anisole (BHA) as reference antioxidant.
There were significant differences (P<0.05) in the antioxidant activity between
the treatments in all measuring methods. The means of antioxidant activity
amounted to 82.66% in T-1, 86.82% in T-2 and 86.98% in BHA. There was
significant relationship between total phenols and antioxidant activity in all
treatments.
Pretreatment is one of the most important stage in olive mill
wastewater (OMWW) plants. Statistically, the cost of chemicals in
pretreatment will be not more than 0.02 €/m³OMWW for some kind of
chemical coagulants such as CaO.
The effect of phenolic compounds were extracted from leaves of Dan
and Toufahi sorts of Syrian olives, spread particularly in Damascus
country, studied on the activity of Guaiacol peroxidise in the celles of
Kolioptyl Zea myse L. Solutions of thre
e different concentrations of
phenolic extracts are used in the work : 25% , 50% and 100%.
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