This current study was conducted on gardenia plant (Gardenia
jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect
of some plant growth regulators on multiplication and rooting, for the aim of
developing a complete in v
itro microprpagation system of the studied cv.
Results demonstrated that, the best medium for in vitro micropropagation of
the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA
with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73
cm, and average leaf number of 4.36. These shoots were then transferred to a
rooting media containing a half-strength of the multiplication basal media with
NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of
97% with average root number of 9.67, and average root length of 5.35 cm, and
average stem length of 9.80 cm, and average leaf number of 5.07. Increasing
NAA concentration in rooting media to 1.5 mg/L resulted in increasing root
length average to 6.28 cm with significant difference in comparison to the
previous treatment. Rooted Plantlets were acclimatized gradually in a growth
room conditions to ex vitro conditions with 85 % efficiency.
The Madagascar periwinkle (Catharanthus roseus) produces numerous
indole alkaloids, several of which have an important pharmaceutical uses such
as ajmalicine. The relationship between hypoxia and ajmalicine production in a
cell medium culture of C
atharanthus roseus were investigated during the cycle
of cell culture, as well as the growth in fresh and dry matter. The results show
that the lack of oxygenation in C20D cells provokes a very strong inhibition in
accumulation of the alkaloids and of other possible substances. This can be
explained by the absence of some downstream enzymes involved in the
biosynthesis chain of alkaloids or by the blockage of the upstream terpenes to
the production stage. Reoxygenation did not restore hypoxia effect on the
alkaloid accumulation.
Moreover, the present study showed that the addition of the loganin in the
4th day, in the cell culture medium subjected to hypoxia restored the alkaloid
production.
Also, the results show that the BAP increases the ajmalicine production. The
effect of hypoxia was not shown in the presence of cytokinine. The BAP can
without doubt decrease the effects of the hypoxia and increases the ajmalicine
production.
Potato (Solanum tuberosum L.) is among the most
economically important crops world-wide and in Syria
as well. It is highly responsive to different tissue culture
techniques.
In vitro culture of potato cv. 'Draga' (mid-early) was
established thro
ugh meristem tip culture.
