This current study was conducted on gardenia plant (Gardenia
jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect
of some plant growth regulators on multiplication and rooting, for the aim of
developing a complete in v
itro microprpagation system of the studied cv.
Results demonstrated that, the best medium for in vitro micropropagation of
the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA
with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73
cm, and average leaf number of 4.36. These shoots were then transferred to a
rooting media containing a half-strength of the multiplication basal media with
NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of
97% with average root number of 9.67, and average root length of 5.35 cm, and
average stem length of 9.80 cm, and average leaf number of 5.07. Increasing
NAA concentration in rooting media to 1.5 mg/L resulted in increasing root
length average to 6.28 cm with significant difference in comparison to the
previous treatment. Rooted Plantlets were acclimatized gradually in a growth
room conditions to ex vitro conditions with 85 % efficiency.
This study aimed to investigate Four levels of IBA concentration: (0
(control), 1000, 2000, 4000 ppm) and three kinds of rooting media: sand,
peatmoss, sand+sawdust in ratio of 1:1 in a factorial complete random design.
The results indicated that
the concentration of 2000 ppm was superior at
rooting percentage 76%, root number (20 roots) and root length 13 cm over the
other levels of concentration. Besides, the media of (Sand+Sawdust) was
superior at rooting percentage 72%, rooting number (19 roots), root length 11
cm, physical and chemical charactaristics and the charactaristics of the rooted
cuttings.
This study has been conducted to show the influence of some factors to the
rooting of semi-wooden cuttings of boxwood Boxus suffruticosa L. in order to
know the best concentration of indol biotryc acid (IBA) and the best rooting
media for the root
ing of boxwood cuttings .
The experiment was designed in the sub-plots method and semi-wooden
Boxwood cuttings were used. The cuttings were treated with three
concentrations of IBA: 3000, 5000, 10000 p.p.m. using fast dipping. Then the
cuttings were planted (distributed) in five rooting media, namely: sand, peatmoss,
khaffan, (sand+peat-moss) in the rate of (1:1), (khaffan+ perlite) in the
rate of 1:5 respectively. under protected conditions. The experiment has
continued for 8 weeks.