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This current study was conducted on gardenia plant (Gardenia jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect of some plant growth regulators on multiplication and rooting, for the aim of developing a complete in v itro microprpagation system of the studied cv. Results demonstrated that, the best medium for in vitro micropropagation of the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73 cm, and average leaf number of 4.36. These shoots were then transferred to a rooting media containing a half-strength of the multiplication basal media with NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of 97% with average root number of 9.67, and average root length of 5.35 cm, and average stem length of 9.80 cm, and average leaf number of 5.07. Increasing NAA concentration in rooting media to 1.5 mg/L resulted in increasing root length average to 6.28 cm with significant difference in comparison to the previous treatment. Rooted Plantlets were acclimatized gradually in a growth room conditions to ex vitro conditions with 85 % efficiency.
This study aimed to investigate Four levels of IBA concentration: (0 (control), 1000, 2000, 4000 ppm) and three kinds of rooting media: sand, peatmoss, sand+sawdust in ratio of 1:1 in a factorial complete random design. The results indicated that the concentration of 2000 ppm was superior at rooting percentage 76%, root number (20 roots) and root length 13 cm over the other levels of concentration. Besides, the media of (Sand+Sawdust) was superior at rooting percentage 72%, rooting number (19 roots), root length 11 cm, physical and chemical charactaristics and the charactaristics of the rooted cuttings.
This study has been conducted to show the influence of some factors to the rooting of semi-wooden cuttings of boxwood Boxus suffruticosa L. in order to know the best concentration of indol biotryc acid (IBA) and the best rooting media for the root ing of boxwood cuttings . The experiment was designed in the sub-plots method and semi-wooden Boxwood cuttings were used. The cuttings were treated with three concentrations of IBA: 3000, 5000, 10000 p.p.m. using fast dipping. Then the cuttings were planted (distributed) in five rooting media, namely: sand, peatmoss, khaffan, (sand+peat-moss) in the rate of (1:1), (khaffan+ perlite) in the rate of 1:5 respectively. under protected conditions. The experiment has continued for 8 weeks.
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