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Register a new userEffect of Some Growth Hormones on Multiplication and Rooting in vitro Micro- Propagated of Gardenia Plant (Gardenia jasminoides.L.) cv. ellis.
تأثير بعض هرمونات النمو في إكثار نبات الغاردينيا (jasminoides Gardenia) صنف ellis و تجذيره في المخبر الزجاجي (In vitro)
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Damascus University ورقة بحثية
Publication date
2010
fields
Groves
and research's language is
العربية
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أجري هذا البحث على نبات الغاردينيا (.L jasminoides Gardenia) صنف ellis الذي تـم إكثـاره مخبرياً في الزجاج (vitro in) بهدف دراسة تأثير بعض منظمات النمو في مرحلة الإكثار و التجـذير بغيـة تطوير تقنية كاملة للإكثار الخضري الدقيق للصنف المدروس. أوضحت النتائج أن أفضل وسط زرع للإكثـار 1 mg/L BA + 0.1 mg/L IBA: علـى و المحتـوي MS وسـط هو المدروس للصنف الدقيق المخبري و بمعدل اكثار بلغ 33.5 نمواً خضرياً جديداً، مع متوسط طول بلغ 73.4 سم، و عـدد أوراق بلـغ 36.4. نقلت بعدها هذه النموات إلى أوساط التجذير المحتوية على نصف تركيز العناصر الكبـرى مـن الوسـط الأساس المستخدم للإكثار مع الأكسين NAA بتركيز L/1mg حيث تم الحصول على أعلى معدل تجـذير بفعالية بلغت 97 % مع أكبر عدد للجذور بلغ 67.9 ، و متوسط طول جذري بلغ 35.5 سم، و متوسط طول ساق بلغ 80.6 سم، و متوسط عدد أوراق بلغ 07.5 . و أدت زيادة تركيز الأوكسين في أوساط التجذير إلى L/mg 5.1 إلى ازدياد متوسط طول الجذور إلى 28.6 سم و بفرق معنوي مقارنة بالمعاملة السابقة. تـم تقسية النموات المجذرة تدريجياً في شروط غرفة النمو لشروط الوسط الخارجي و بنـسبة نجـاح بلغـت %85.
This current study was conducted on gardenia plant (Gardenia jasminoides.L.) cv. ellis. which was micropropagated in vitro to study the effect of some plant growth regulators on multiplication and rooting, for the aim of developing a complete in vitro microprpagation system of the studied cv. Results demonstrated that, the best medium for in vitro micropropagation of the studied cv. was MS medium supplemented with 1 mg/L BA + 0.1 mg/L IBA with a multiplication rate of 5.33 new shoots, with average shoot height of 4.73 cm, and average leaf number of 4.36. These shoots were then transferred to a rooting media containing a half-strength of the multiplication basal media with NAA at a concentration of 1mg/L which resulted in highest rooting efficiency of 97% with average root number of 9.67, and average root length of 5.35 cm, and average stem length of 9.80 cm, and average leaf number of 5.07. Increasing NAA concentration in rooting media to 1.5 mg/L resulted in increasing root length average to 6.28 cm with significant difference in comparison to the previous treatment. Rooted Plantlets were acclimatized gradually in a growth room conditions to ex vitro conditions with 85 % efficiency.
References used
Abdallah, G.; Alkateeb, A.and Seragali, M. (2003). Effect of some plant growth regulatores on in vitro micro propagation of gardenia plant .Soltan Kaboos Univ. J.of Agric.and Sealife Scie.18 (1) : 35-40
AL-Juboory, K. H.; Skirvin, R. M. and Williaams, D. J. (1998). Callus induction and adventitious shoot regeneration of gardenia (Gardenia jasminoides Ellis) leaf explants. Scienta horticulturae,72(4):171-178
Bradshow, J. (2003). Gardenias. Florida Cooperative Extension Service, University of Florida . CIR, 1098
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Read More
This current study was carried out on B41 grape rootstock
micropropagated in vitro at the General Commission for Scientific Agricultural
Research (GCSAR), Syria to study the effect of different concentrations of
sodium chloride on the multiplicati
on and rooting stages. The results showed
that the highest average of survival plants (98%), the number of new buds
(8.43) and plant length (8.58 cm) were obtained in the control treatment after 4
weeks from multiplication stage. The treatment with 50 mM of sodium chloride
led to reduce the multiplication rates with significant differences, while the
lowest plant multiplication had occurred with 100 mM of sodium chloride and
no survival plants were remained by the treatment with 150 mM of sodium
chloride during multiplication stage. The highest rooting rates (% 85.30), the
number of roots (4.67) and root length (6.28 cm) were also obtained in the
control treatment after 4 weeks from rooting stage. The treatment with 50 mM
of sodium chloride led to reduce the rooting process with significant
differences. The lowest rooting results were observed with 100 mM of sodium
chloride and no rooting rate was observed when plants were treated with 150
mM of sodium chloride.
Gardenia jasminoides is considered as a plant of tropical and suptrofical regions.
This plant has a great importance in Germany and Syria. Despite that this research has
been done in Germany, it is possible to generalize it for Syria as it was cond
ucted in
stable laboratory conditions. The experiment was conducted in the environment of
greenhouse no. 5 at the Higher Gardening Institute- Branch of Ornamental plants in
Berlin. Cuttings imported from Denmark were used after being stored in an atmosphere
of high relative humidity reaching 95-100%.
This research was conducated on Ceratonia siliqua L. in vitro to study the effect of
some growth regulators in both multiplication and rooting stage. And determine the best
concentrations wich lead the highest rate of multiplication (interms of number of shoots
and length), and the best rooting (interms of percentage of rooting, number of roots and
length).
In this study, the effect of different concentrations of sodium chloride
(NaCl) (0, 50, 100, 150 mM) on some physiological characteristics and some
growth indicators of SO4 grape rootstock propagated in vitro was studied at the
laboratories of Gen
eral Commission for Scientific Agricultural Research
(GCSAR), Damascus/Syria. Results indicated that the increase of NaCl
concentration up to 100 mM in culture medium led to reduce the growth
indicators (survival plants, average of the plant length and average number of
buds) with significant differences when compared with the control plants after
4 weeks from culturing on multiplication media, while treatment with 150 mM
of sodium chloride led to death of all plants. Using the concentrations 50 and
100 mM of NaCl also caused decreasing the total chlorophyll content in the
leaves.
This research aims to study the proteins transformation in zea maize plant
seedlings cells, (GHOTA 82) under the influence of phyto hormone Auxin &
grothregulators 2,4-D and IBA.
Maize plant seeds were germinated in water medium for six days in
d
arkness and temperature of 25 ºC.
The quantity different groups of proteins, were analyzed in the stem cells of
the developing seedling mesochotyles after incupation in darkness water and in
different solutions of auxin, 2, 4D & IBA (in concentration 50 mg/l) for 20
hours in 26 ºC.
The results showed that had inhibition effects on hydrolysis of protein
groups in zea maize plant seedlings cells.
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