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Register a new userStuding the Effect some of plant growth regulators on the activity of hydrolysis and oxidation enzymes in the seedling of Zea mays stems cells
دراسة تأثير بعض منظمات النمو في فعالية عدد من إنزيمات الحلمهة و إنزيمات الأكسدة في خلايا ساق نبات الذرة الصفراء Zea may
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جرى في البحث تحري تأثير كلٍ من منظمات النمو النباتية الآتيـة: حمـض أنـدول الخـل (IAA ( و حمض 4.2 -ثنائي كلورو فينوكسي الخل (D-4,2 ،( و حمض اندول الزبدة (IBA ،( في فعالية كل مـن إنزيمي الحلمهة: α-الأميلاز و الريبونوكليآز و في فعالية ثلاثة من إنزيمات الأكسدة و الإرجاع: الكاتـالاز و الغوياكول بيروكسيداز و الأسكوربات أوكسيداز في خلايا مقاطع ساق نبات الذرة الـصفراء مـن نـوع غوطة- 82 المستنبتة مدة ستة أيام في الظلام و بدرجة حرارة 26ºC؛ و ذلك بعد20 ساعة مـن عزلهـا و حضنها بالماء و في محاليل منظمات النمو المبينة أعلاه بتركيز (50 ملغ/ل) و في الدرجـة 30ºC .و قـد أظهرت النتائج التي تم الوصول إليها أن حضن المقاطع بالمـاء المقطـر أو بمحاليـل منظمـات النمـو المدروسة بتركيز (50 ملغ/ل)، قد أثر تأثيراً متفاوتاً في فعالية الإنزيمات المدروسة.
Investigate studied the activity of some enzymes in zea maize plant seedlings cells, (Ghota 82) under the influence of phytohormone Auxin & growth regulators 2,4-D and IBA. Maize plant seeds were germinated in water medium for six days in darkness and temperature of 260C. Then stem cells of the developing seedling were incupated in darkness with water or in different solutions of auxin, 2,4-D & IBA (50 mg/l) for 20 houre in 30 0C. Then magared the activity of α-Amylase, RNAase, Catalayse, Guaiacol Peroxidase, Ascorbate oxidase in the stem cells The results showed that the incupation has defferent effects.on hydrolysis and oxidative enzymes in zea maize plant seedlings cells.
References used
Junqi Wang, Yubing Li, Sze Wan Lo, Stefan Hillm, Sam Uel S. M. Sun, Sun, David G. Robinson, and Liwen Jiang. 2007. Protein Mobilization in Germinating Mung Bean Seeds Involves Vacuolar Sorting Receptors and Multivesicular Bodies, Sciences, University of Heidelberg, plant physiology 143:1628-1639
Marihelen Glass, Rick Parker. 2009. Fundamentals of plant science. Delmar, Cengage Learning
Husen, A. 2008. Clonal propagation of Dalbergia sissoo Robx. And associated metabolic changes during adventitious root primordium development. New Forests 36,13-27
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This research aims to study the proteins transformation in zea maize plant
seedlings cells, (GHOTA 82) under the influence of phyto hormone Auxin &
grothregulators 2,4-D and IBA.
Maize plant seeds were germinated in water medium for six days in
d
arkness and temperature of 25 ºC.
The quantity different groups of proteins, were analyzed in the stem cells of
the developing seedling mesochotyles after incupation in darkness water and in
different solutions of auxin, 2, 4D & IBA (in concentration 50 mg/l) for 20
hours in 26 ºC.
The results showed that had inhibition effects on hydrolysis of protein
groups in zea maize plant seedlings cells.
In this work we study the kinetic of Catalase and Guaiacol peroxidase and the effect of : pH, temperature, enzyme concentration and substrate concentration on the activity of these enzymes in the mesocotyles of Zea mays ( Ghoto82 ) 6 day old.
This research aims to study the effects of some oxidoreductase enzymes
activity in the seedlings of maize plant stem cells, which Cultured in the present
of the following cations: Na+, K+, Mg2+, and Ca2+.
(GHOTA 82) yallow corn seeds were used, an
d cultured for six days in
water and in solution of cations with different concentrations.
In brief, preparation and titration processes consist of extraction of
enzymatic solutions from plant stem cells seedlings by buffer solutions (at
appropriate PH for each enzyme).
The results show that the activity of used enzymes increases with the
increasing concentrations of the used cations from 1 to 6 g/l by comparison with
those of cultured samples in water.
Besides the difference in the effect of cations was limited by the
concentrations range between 6-9 g/ l.
This study was carried out in the farm of the Faculty of Agriculture
University of Damascus during the growing season 2014, to study the
productivity of the maize cultivar ("Gouta 82) and the hybrids (Basel
1 and Basel 2) under the influence of tw
o plant densities 17.53
thousand plant. ha-1 (70x20cm) and 53.11 thousand plant. ha-1
(70x40cm).
The aim of this research is to determine the effect of some heavy metal ions
on total protien metabolism in seeds and seedlings of maize plant (GHOTA 82)
and study the changes in the morphological specifications of plant, after
cultivating for 6 d
ays in solutions at different concentrations of water and
heavy metal ions Ni2+, Cu2+, Zn2+, Cd2+, Hg2+ and Pb2+.
The results showed that heavy metal ions have inhibitory effect on total
protien metabolism, which decreased their amount in seedlings, While
accumulated in the seeds. This inhibitory effect depends on the type and
concentration of heavy metal ions.
The results also showed morphological changes in the maize plant seedlings,
which decreased their growth due to the effect of these ions.
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