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This research aims to study the proteins transformation in zea maize plant seedlings cells, (GHOTA 82) under the influence of phyto hormone Auxin & grothregulators 2,4-D and IBA. Maize plant seeds were germinated in water medium for six days in d arkness and temperature of 25 ºC. The quantity different groups of proteins, were analyzed in the stem cells of the developing seedling mesochotyles after incupation in darkness water and in different solutions of auxin, 2, 4D & IBA (in concentration 50 mg/l) for 20 hours in 26 ºC. The results showed that had inhibition effects on hydrolysis of protein groups in zea maize plant seedlings cells.
Investigate studied the activity of some enzymes in zea maize plant seedlings cells, (Ghota 82) under the influence of phytohormone Auxin & growth regulators 2,4-D and IBA. Maize plant seeds were germinated in water medium for six days in darknes s and temperature of 260C. Then stem cells of the developing seedling were incupated in darkness with water or in different solutions of auxin, 2,4-D & IBA (50 mg/l) for 20 houre in 30 0C. Then magared the activity of α-Amylase, RNAase, Catalayse, Guaiacol Peroxidase, Ascorbate oxidase in the stem cells The results showed that the incupation has defferent effects.on hydrolysis and oxidative enzymes in zea maize plant seedlings cells.
Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus communis L.) grown in the field under natural conditions at Damascus countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected by 70% Ethanol an d Clorox containing 5.25 % Sodium Hypoclorite with a drop of Tween 20 for different periods and concentrations before being placed onto MS basal medium. Established cultures were then transferred onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of 12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44 μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
A successful and detailed in vitro propagation system for rapid micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has been developed. Shoot tips and axillary buds excised from field-grown trees were used as explants, which were surface-disinfected with mercury bichloride, or with solution of sodium or calcium hypochlorite followed by three rinses with sterile distilled water.
A study on the propagation of female kiwi trees (Hayward variety) by wooden cuttings, using plant growth regulators, was conducted at Latakia Agricultural Research Centre during the seasons 2009, 2011 and 2012. Two dates for collecting cuttings (J anuary and February) from kiwi trees which were selected. On each date, the cuttings were divided into three groups according to cutting location on the shoot (basal, middle and apical). The NAA and IBA growth regulators were applied at several concentrations, in addition to two treatments of the mixture of both growth regulators.
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