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Register a new userEffect of plant growth regulators on metabolism of proteins in the seedling of Maize plant stems cells
تأثير بعض منظمات النمو النباتية في استقلاب البروتينات في خلايا ساق نبات الذرة الصفراء
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درس في البحث المقدم تأثير مجموعة من منظمات النمو النباتية و هي حمض انـدول الخـل (IAA ( و حمض 2,4 ثنائي كلورو فنوكسي الخل (D-4,2 ،(و حمـض انـدول الزبـدة (IBA ،(فـي اسـتقلاب البروتينات الكلية في خلايا مقاطع ساق بادرات نبات الذرة الصفراء من نوع غوطة- 82 المستنبتة مـدة ستة أيام في الظلام و بدرجة حرارة 25ºc ،و ذلك بعد20 ساعة من عزلها و حضنها بالماء و فـي محاليـل منظمات النمو المبينة أعلاه بتركيز (50 ملغ/ل). كما درست التغيرات البنيوية الحاصلة في تراكيز كل من المجموعات البروتينية الأربع المكونة لبروتينات خلايا الساق فـي نبـات الـذرة، و هـي الألبومينـات، الغلوبولينات، البرولامينات، و الغلوتيلينات. بينت النتائج التي تم الوصول إليها أن تركيز البروتينات الكلي يتناقص عند عزل مقـاطع البـادرات و حضنها سواء بالماء المقطر أو بمحاليل منظمات النمو المدروسة بتركيز (50 ملغ/ل)، إلا أن منظمـات النمو عملت على التقليل من معاناة مقاطع النبات المعزولة؛ و ذلك بتثبيطها لعملية تفكك بروتينات الخلايـا في البادرات المحضونة مقارنة بما تعرضت له هذه البروتينات في المقاطع المحضونة بالماء المقطر.
This research aims to study the proteins transformation in zea maize plant seedlings cells, (GHOTA 82) under the influence of phyto hormone Auxin & grothregulators 2,4-D and IBA. Maize plant seeds were germinated in water medium for six days in darkness and temperature of 25 ºC. The quantity different groups of proteins, were analyzed in the stem cells of the developing seedling mesochotyles after incupation in darkness water and in different solutions of auxin, 2, 4D & IBA (in concentration 50 mg/l) for 20 hours in 26 ºC. The results showed that had inhibition effects on hydrolysis of protein groups in zea maize plant seedlings cells.
References used
Onwuka, C. Frank, Ikewuchi, C. Catherin. Jude & Ayalogu, O. Edward. 2009. Investigation on the effect of Germination on the Proximate Composition of African Yam Bean. Journal of Applied Sciences & Environmental.Vol. 13, No. 2, June .pp.59-61
Malo. A, Malek. A. 2010. The influence of Thuja oil, α- pinene and α- thujone emulsions on the proteins and lipids metabolism in the seedlings maize plant stems cells,Aleppo University Journal No73; 2010
Junqi Wang, Yubing Li, Sze Wan Lo, Stefan Hillm, Sam Uel S. M. Sun, Sun, David G. Robinson, and Liwen Jiang. 2007. Protein Mobilization in Germinating Mung Bean Seeds Involves Vacuolar Sorting Receptors and Multivesicular Bodies, Sciences, University of Heidelberg, plant physiology 143:1628-1639
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Investigate studied the activity of some enzymes in zea maize plant seedlings
cells, (Ghota 82) under the influence of phytohormone Auxin & growth
regulators 2,4-D and IBA.
Maize plant seeds were germinated in water medium for six days in
darknes
s and temperature of 260C.
Then stem cells of the developing seedling were incupated in darkness with
water or in different solutions of auxin, 2,4-D & IBA (50 mg/l) for 20 houre in
30 0C. Then magared the activity of α-Amylase, RNAase, Catalayse, Guaiacol
Peroxidase, Ascorbate oxidase in the stem cells
The results showed that the incupation has defferent effects.on hydrolysis
and oxidative enzymes in zea maize plant seedlings cells.
The aim of this research is to determine the effect of some heavy metal ions
on total protien metabolism in seeds and seedlings of maize plant (GHOTA 82)
and study the changes in the morphological specifications of plant, after
cultivating for 6 d
ays in solutions at different concentrations of water and
heavy metal ions Ni2+, Cu2+, Zn2+, Cd2+, Hg2+ and Pb2+.
The results showed that heavy metal ions have inhibitory effect on total
protien metabolism, which decreased their amount in seedlings, While
accumulated in the seeds. This inhibitory effect depends on the type and
concentration of heavy metal ions.
The results also showed morphological changes in the maize plant seedlings,
which decreased their growth due to the effect of these ions.
Objective of this work was to investigate how plant density and nitrogen
rate affects the growth , yield and its components in maize (Bassel 2 hybrid).
The experiment was carried out, at the agricultural research centre in Snoubar
Jableh, during t
he 2008 and 2009 seasons, included 12 treatments which were
the combination three plant population densities 71428/ha, 57142/ha, and
47619/ha and four N rates (0, 60, 120 and 180 kg/ha). The experimental design
was a split-plot, with different densities as main plots, randomized in three
complete blocks, and the fertilization as subplots. The data were analyzed by
using “Mstat” statistical package. Increasing planting population density
delayed anthesis and silking date, increased the anthesis-silking interval, plant
height, ear height, grain yield, agronomic nitrogen efficiency and nitrogen use
efficiency, but decreased ear leaf area, chlorophyll content, 1000 seed weight,
number of grains/ear, shelling percentage (%), and grain protein content.
Nitrogen fertilizer decreased tassilling and silking date, and nitrogen use
efficiency, increasing plant and ear height, ear leaf area, chlorophyll content,
number of grains/ear, ear weight,1000-grains weight, Shelling percentage,
grain yield up to 120 kg N/ha, agronomic nitrogen efficiency and grain protein
content.
Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus
communis L.) grown in the field under natural conditions at Damascus
countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected
by 70% Ethanol an
d Clorox containing 5.25 % Sodium Hypoclorite
with a drop of Tween 20 for different periods and concentrations before being
placed onto MS basal medium. Established cultures were then transferred onto
MS basal medium containing a combination of growth regulators at different
concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM
or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of
12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44
μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
This study was carried out in the farm of the Faculty of Agriculture
University of Damascus during the growing season 2014, to study the
productivity of the maize cultivar ("Gouta 82) and the hybrids (Basel
1 and Basel 2) under the influence of tw
o plant densities 17.53
thousand plant. ha-1 (70x20cm) and 53.11 thousand plant. ha-1
(70x40cm).
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