Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus
communis L.) grown in the field under natural conditions at Damascus
countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected
by 70% Ethanol an
d Clorox containing 5.25 % Sodium Hypoclorite
with a drop of Tween 20 for different periods and concentrations before being
placed onto MS basal medium. Established cultures were then transferred onto
MS basal medium containing a combination of growth regulators at different
concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM
or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of
12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44
μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
This present study was conducted to develop a detailed in vitro propagation
system for the medicinal shrub Capparis spinosa L.
Single nodes with one bud and a small part of stem of 1-1.5 cm long were
used as initial explants which were collected f
rom a shrubs grown under field
conditions at Damascus suburb., (Doumar). Explants were surface-disinfected
by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or
HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100
ml disinfectant solution, where after, they were placed onto MS basal medium
containing a combination of growth regulators at different concentrations (BA
at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the
growth room at 23±1 c and light intensity of 3000 lux at the cultures level.
Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks
on the optimal MS medium (MS+8.88μM BA+0.49μM IBA).
The described method has potential to produce large numbers of plantlets
within a short period of time to expand its cultivation for medicinal uses.
A successful and detailed in vitro propagation system for rapid
micropropagation of three apple rootstocks: MM ١٠٦, EM ٧ and M ٢٦ has
been developed. Shoot tips and axillary buds excised from field-grown trees
were used as explants, which were surface-disinfected with mercury bichloride,
or with solution of sodium or calcium hypochlorite followed by three rinses
with sterile distilled water.
