Subscribe to the gold package and get unlimited access to Shamra Academy
Register a new userEffect of Plant Growth Regulators on In vitro micropropagation of Myrtle (Myrtus communis L.)
تأثير منظمات النمو في الإكثار الدقيق لنبات الآس العطري Myrtus communis L
1016
2 18
0
(
0
)
Authors
سليم حسين زيد( باحث )
Created by
Shamra Editor
Ask ChatGPT about the research
اِستُخدمت عقل صغيرة بطول 5.1 سم (العقدة مع برعمها) من أغصان إحدى أشجار الآس العطـري في منطقة عين الفيجة بدمشق، حيث عقمت بالكحول 70 % و بتراكيز متعددة مـن الكلـوركس التجـاري (المحتوي على هيبوكلوريت الصوديوم بتركيز 25.5 %كمادة فعالة) مع إضافة بضع قطرات من محلـول توين 20 خلال مدد زمنية مختلفة. بعد ذلك استنبتت على وسط موراشيج و سكوج (MS) الـذي أضـيف إليه، بغية الإكثار و التجذير، تراكيز متنوعة من منظمات النمو الآتية: البنزيـل أدنـين (BA) و نفتـالين حمض الخل (NAA) أو أندول حمض الزبدة (IBA) و حمض الجبريللين (GA3) .
Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus communis L.) grown in the field under natural conditions at Damascus countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected by 70% Ethanol and Clorox containing 5.25 % Sodium Hypoclorite with a drop of Tween 20 for different periods and concentrations before being placed onto MS basal medium. Established cultures were then transferred onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of 12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44 μM BA with 1.47 μM IBA and GA3 at 0.58 μM.
References used
Gardeli C., Papageorgiou V., Mallouchos A., Theodosis K., Komaitis M. (2008). Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts. Science Direct , Food Chemistry 107: 1120-1130
Jones O., pontikis A., Hopgood, M. (1979). Propagation in vitro of five apple scion cultivares . J. Hort. Sci . 54 (2): 155-185
Khosh-Khvl, M., Shekafandeh, A., Azarakhsh, H. (1984). Micropropagation of Myrtle. Scientia Horticulturae, 22 (2/1) :134-146
rate research
Read More
This research was carried out to determine the quantity of essential oil extracted
from myrtle Myrtus communis L leaves, by hydro distillation, and to identify the
major and minor components, besides to determine its quantities in the essential oil
by Gas Chromatography-Mass Spectrometry GC-MS. The samples were collected
on November 2013 from eight different climatic locations (Aleppo, Zarzour, Alzenea,
Katra AL-Rayhan, Al- Rayhana, Kasab, Mashta al-Holou and Mosiaf). The
quantity and composition of the essential oil were estimated on the basis of the
leaves dry weight. Oil quantity varied between the studied genotypes from different
locations, which range from 0.46 to 0.55 ml, with non-significant differences.
Eighteen compounds were identified in myrtle essential oil, and there were
significant differences in 13 compounds. 1,8-Cineole performed the highest rate
(18.85%) compared with other components, followed by α-Pinene (16.93%). While
the lowest percentage was (0.31%) of Myrcene. The
Ziziphora canescens is a species of important medicinal plants in Syria due
to its medicinal properties as antibiotic, flavors and spices in various foods.
This plant is important, especially in folk medicine in some areas (Kalamoon)
on the one ha
nd, and retreat of its spread which may lead eventually to its
extinction on the other hand, so a protocol for rapid micropropagation has
been developing by using lateral and apical buds on nutrient media MS
supplemented with different types and concentrations of plant growth
regulators.
This research was conducated on Ceratonia siliqua L. in vitro to study the effect of
some growth regulators in both multiplication and rooting stage. And determine the best
concentrations wich lead the highest rate of multiplication (interms of number of shoots
and length), and the best rooting (interms of percentage of rooting, number of roots and
length).
Olive trees are grown in many regions of Syria, especially in wet regions
where the olive knot disease (Pseudomonas savastanoi pv.savastanoi ) prevails
.Symptoms similar to those found on olive trees have been observed on myrtle
shrubs (Myrtus com
munis) growing naturally in some olive-grown regions. This
study aimed at identifying the pathogen isolated from myrtle plant, and testing
the pathogenicity of these isolates on olive trees. Morphological, biochemical
and serological tests of bacteria isolated from myrtle showed similarity to
those from olive and other hosts. Pathogenicity tests showed that the myrtle
isolates were pathogenic on both myrtle and olive trees. Myrtle could be
considered as a source of inoculum for the olive knot disease.
This investigation was conducted on Ru140 grape rootstock at the General
Commission for Scientific Agricultural Research (GCSAR), Damascus with the
aim of in vitro vegetatively micropropagation using some plant growth
regulators on multiplication
and rooting to determine the best combinations
and concentrations of plant growth regulators that result in the best
multiplication rate, and best rooting crekeria (rate and roots number and
length). Results demonstrated that, the best medium for in vitro
micropropagation of the studied rootstock was the modified MS medium
supplemented with 4.44 μM BA + 0.49 μM IBA with multiplication rate of 7.72
new shoots every 4 weeks, and shoots lengt of 5.54 cm. These shoots were
transferred for 4 weeks to among elongation medium containing the same
medium with the addition of Kinetine at a concentration of 2.22 μM instead of
BA which led to a shoot elongation rate of 7.87 cm, then these shoots were
transferred to rooting medium for rooting, It was shown that using auxin IBA
at a concentration of 4.44 μM resulted at the highest rate of rooting (87%) with
the largest number of roots (7.56) when using the auxin IBA concentration μM
4.44 compared with the rest of other transactions and with the control as well.
However, The highest length of roots (6.29 cm) was observed on medim
contained lower IBA concentration (2.22 μM). Rooted Plants were acclimatized
gradually to ex vitro conditions with 70 % efficiency.
Log in to be able to interact and post comments
comments
Fetching comments
Sign in to be able to follow your search criteria
