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Effect of Plant Growth Regulators on In vitro micropropagation of Myrtle (Myrtus communis L.)

تأثير منظمات النمو في الإكثار الدقيق لنبات الآس العطري Myrtus communis L

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 Publication date 2011
and research's language is العربية
 Created by Shamra Editor




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Single nodes and axillary buds excised from adult trees of Myrtle (Myrtus communis L.) grown in the field under natural conditions at Damascus countryside (Ain Elfiegh) were used as primary explants, which were surfacedisinfected by 70% Ethanol and Clorox containing 5.25 % Sodium Hypoclorite with a drop of Tween 20 for different periods and concentrations before being placed onto MS basal medium. Established cultures were then transferred onto MS basal medium containing a combination of growth regulators at different concentrations (BA at 2.22 and 4.44 μM) each with NAA at 0.54, 1.62, 5.4 μM or 1.47 μM IBA with GA3 at 0.58 μM for all tratments. Multiplication rate of 12.8-fold was achieved every 4 weeks on MS medium supplemented with 4.44 μM BA with 1.47 μM IBA and GA3 at 0.58 μM.


Artificial intelligence review:
Research summary
تتناول هذه الدراسة تأثير منظمات نمو النبات على التكاثر الدقيق لنبات الآس (Myrtus communis L.) في المختبر. استخدمت العقد المفردة والبراعم الإبطية المأخوذة من أشجار الآس البالغة كنماذج أولية، وتم تطهيرها باستخدام الإيثانول بنسبة 70% وكلوروكس الذي يحتوي على 5.25% من هيبوكلوريت الصوديوم مع إضافة قطرة من توين 20 لفترات وتركيزات مختلفة قبل وضعها على وسط MS الأساسي. بعد ذلك، تم نقل الثقافات إلى وسط MS الأساسي المحتوي على مجموعة من منظمات النمو بتركيزات مختلفة. تم تحقيق معدل تكاثر يبلغ 12.8 ضعف كل 4 أسابيع على وسط MS المضاف إليه 4.44 ميكرومول من BA مع 1.47 ميكرومول من IBA و0.58 ميكرومول من GA3. تم تحقيق التجذير خلال 4 أسابيع على وسط يحتوي على 4.9 ميكرومول من IBA بكفاءة 95%. تم نقل النباتات المجذرة إلى أواني تحتوي على خليط من الخث والبيرلايت بنسبة 2:1 وتم تكييفها تدريجياً مع الظروف الحقلية خلال 4-5 أسابيع مع نسبة بقاء عالية. تشير الطريقة الموصوفة إلى إمكانية إنتاج مئات الآلاف من النباتات المجذرة والمكيفة في فترة زمنية قصيرة.
Critical review
دراسة نقدية: تعتبر هذه الدراسة ذات قيمة كبيرة في مجال تكاثر النباتات في المختبر، حيث تقدم طريقة فعالة وسريعة لإنتاج نباتات الآس بكميات كبيرة. ومع ذلك، هناك بعض النقاط التي يمكن تحسينها. أولاً، لم يتم توضيح تأثير الظروف البيئية المختلفة على نجاح عملية التكاثر والتجذير، مما قد يكون له تأثير كبير على النتائج في التطبيقات العملية. ثانياً، لم يتم مناقشة التكلفة الاقتصادية للطريقة المستخدمة، وهو عامل مهم يجب مراعاته عند التفكير في تطبيق هذه الطريقة على نطاق واسع. وأخيراً، يمكن أن تكون الدراسة أكثر شمولية إذا تم تضمين تجارب ميدانية لتقييم أداء النباتات المكاثرة في ظروف الحقل المختلفة.
Questions related to the research
  1. ما هو الهدف الرئيسي من هذه الدراسة؟

    الهدف الرئيسي هو دراسة تأثير منظمات نمو النبات على التكاثر الدقيق لنبات الآس (Myrtus communis L.) في المختبر.

  2. ما هي المواد المستخدمة لتطهير النماذج الأولية؟

    تم استخدام الإيثانول بنسبة 70% وكلوروكس الذي يحتوي على 5.25% من هيبوكلوريت الصوديوم مع إضافة قطرة من توين 20.

  3. ما هو معدل التكاثر الذي تم تحقيقه في الدراسة؟

    تم تحقيق معدل تكاثر يبلغ 12.8 ضعف كل 4 أسابيع.

  4. ما هي نسبة نجاح التجذير التي تم تحقيقها؟

    تم تحقيق نسبة نجاح تجذير تبلغ 95%.


References used
Gardeli C., Papageorgiou V., Mallouchos A., Theodosis K., Komaitis M. (2008). Essential oil composition of Pistacia lentiscus L. and Myrtus communis L.: Evaluation of antioxidant capacity of methanolic extracts. Science Direct , Food Chemistry 107: 1120-1130
Jones O., pontikis A., Hopgood, M. (1979). Propagation in vitro of five apple scion cultivares . J. Hort. Sci . 54 (2): 155-185
Khosh-Khvl, M., Shekafandeh, A., Azarakhsh, H. (1984). Micropropagation of Myrtle. Scientia Horticulturae, 22 (2/1) :134-146
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