The research was carried out in the biotechnology Laboratory at the
Faculty of Agriculture, Damascus University, in cooperation with the General
Commission For Scientific Agricultural Research (GCSAR), during the year
2012. Eleven genotypes of cul
tivated chickpea were planted to determine their
genetic diversity and the degree of genetic similarity using the ISSR technique.
16 primers were used for this purpose. Results indicated that Nine primers
proved their effectiveness in showing polymorphism among the genotypes
examined and the primers gave a total of 91 alleles with a polymorphic
percentage of 93.4%. The number of bands for each primer varied from a
minimum of 2 bands for the primers ISSR7-ISSR13 to a maximum of 16 bands
for the primer ISSR11, with an average of 9.1 bands per a primer. The results
also showed that the degree of genetic similarity ranged between 46 and 78%,
where the highest was 78% between the genotypes of Afghanistan – Morocco,
then 77% between the genotypes Tunisia-Afghanistan and Tunisia- Iran and
followed by Spain- Iran and Spain- Pakistan (50%) and the lowest was 46%
between genotypes Afghanistan- Pakistan.
This research was carried out in the laboratories of the Atomic
Energy Commission Molecular Biology and Biotechnology
Department in Damascus to study the molecular characterization of
five of local and introduced grapes varieties which collected f
rom
the Pome and Grapevine Research Center in Sweida using 20
primer pairs of SSR.
