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Register a new userMolecular characterization of some Atriplex species in Syria
التوصيف الجزيئي لبعض أنواع جنس الرغل Atriplex المنتشرة في سورية
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Damascus University ورقة بحثية
Publication date
2012
fields
Forestry and Ecology
and research's language is
العربية
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Shamra Editor
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نُفّذَ البحث في مخبر التقانات الحيوية التابع للهيئة العامة للبحوث العلمية الزراعية خـلال الموسـم 2010-2011 . و قد هدف إلى دراسة التنوع الوراثي لعشرين مدخلاً نباتياً تتبع لسبعة أنواع من الرغـل تنتشر في سورية و تحديد درجة القرابة الوراثية فيما بينها؛ و ذلك باسـتخدام تقنيـة مـا بين تكراريـات 10 الغـرض لهـذا و اسـتخدم Inter Simple Sequence Repeats) ISSR) البسيطة التسلسلات مرئسات. أثبتت المرئسات المستخدمة فعاليتها في إعطاء تعددية شكلية polymorphic بـين الأنـواع المدروسة و نجم عن استخدامها ما مجموعه 195 أليلاً (قريناً)، و بلغت نسبة هذه التعددية 100 % راوح عدد الحزم لكل مرئس بين 12 حزمة كأقل عدد مع المرئس (4-ISSR) و 27 حزمة كـأعلى عـدد مـع المرئس (862-ISSR) بمتوسط 5.19 حزمة لكل مرئس. أظهر كلّ من التحليل العنقودي و شجرة القرابة الوراثية أن أعلى درجة قرابة وراثية كانت بين المدخلين leucoclada1.A و leucoclada2.A) 64.0) في حين كانت أقل درجة قرابة وراثية بين النـوعين leucoclada3.A و glauca2.A) 10.0) . دلـت النتائج على التنوع الوراثي الكبير للأنواع التابعة لجنس الرغل Atriplex في سورية.
This investigation was carried out at the Laboratory of Biotechnology at General Commission for Scientific Agricultural Research (GCSAR), during the season 2010-2011. The aim of this research was to study the genetic diversity among twenty individual plants of seven species and to determine the degree of genetic similarity using the technique ISSR (Inter Simple Sequence Repeats) and 10 primers were used for this purpose. All primers proved their effectiveness in showing polymorphism between the studied species, primers gave a total 195 allele with a polymorphic percentage 100%. The number of bands for each primer varied from a minimum of 12 bands for the primer (ISSR-4) to a maximum of 27 bands for the primer (ISSR-862) in an average of 19.5 bands for each primer, cluster analysis and Dendrogram showed the highest degree of genetic similarity between accession A.leucoclada1 and A.leucoclada2 (0.64), while it was low between species A.leucoclada3 and A.glauca2 (0.10). Results showed vast genetic diversity among the studied species.
References used
Bornet, B.; Goraguer, F.; Joly, G. and Branchard, M. (2002). Genetic diversity in European and Argentinean cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs). Genome 45: 481-484
Bouda, S.; Haddioui, A.; Baaziz, M.; Del Campo, F.F. and Hernández, L.E. (2006). Genetic diversity characterization of genus Atriplex using RAPD markers. Congrès International de Biochimie. Agadir, 09-12 Mai 2006. 64- 68
Chowdhury, M.A.; Vandenberg, B. and Warkentin, T. (2002). Cultivar identification and genetic relationship among selected breeding lines and cultivars in chickpea (Cicer arietinum L.). Euphytica 127: 317–325
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Ephedra is a dioecious, drought- and frost-resistant, perennial, evergreen shrub
with high medicinal value due to its content of ephedrine alkaloids. It became quite clear throughout
referential studies that this significant medical plant which is spontaneously widespread in Syria and there
have been an increased international demand on it hasn’t been studied yet.
Atriplex is one of good adaptive plants to the Characteristics of drought fragile
environments, and it has high ability to tolerate many ecological stresses such as drought
and high temperature and salinity.
The plant is characterized by its huge
economic values because it has many uses, it’s
one of the high digestibility grazing plants and it is grazing by camels, goats and sheep all
the year, some of them have medicinally uses in repairing the increasing of blood sugar,
the freshness parts of some species use in preparing salads, even they have cook using, and
leaves usually have a salty taste even the plant grow in none salinity soils.
The morphological variety of four species of Atriplex were studying, two of them are
introduced (A. canescens, A. polycarpa) and two have natural spreading in Syria
(A. leucoclada, A. halimus).
The results indicated that biological type, life duration, plants color, fruit bracts
shape and fused of fruit bracts, plant smell, seed color, leaves shape and phyllotaxy are
considered the most morphological characters which show important morphological
differences among the studying species. These can be considered among classification
standards to help distinguish the studying species of Atriplex genus under their natural
spreading land, which consider the natural habitat for this important plant. Based on
studied morphological characters, an identification key for the studying species is
provided.
This research was carried out in molecular biology laboratory
faculty of agriculture /Damascus University during the agriculture
season 2015-2016, to study the genetic diversity and determine the
degree of genetic similarity among eleven 11 cultiv
ated vetch
(Aleppo, Idleb, Kamishly, Hassekeh, Tartous, Lattakia, Homs,
Hama, Sweida, Damascus,Daraa) by using the technique ISSR.
The objective of this study was to characterize and determine the genetic
variation among twelve cultivars and four rootstocks, belonging to Amygdalus
genus in Syria using the simple sequence repeats (SSRs) marker. It was found
that 154 alleles we
re identified by using 26 primer pairs, and the proportion of
the specific ones was 30.52%. In addition, it was revealed that all the studied
SSR loci produced polymorphic alleles. The average of PIC, He and Ho were
0.58, 0.61, 0.31, respectively.
Morphological and molecular characterization for six seedling genotypes of
Pistachio vera L (V1,V2,V3,V4,V5, and V6) in comparison with the most
commercial and wide spread varieties, Ashoury andWhite batoury was carried
out in Swaida province, sou
thern of Syria. Nuts chemical contents (dry
substance, moisture, total sugar and oil content) for both fresh and dry nuts
weight were also evaluated.
Seedling genotypes showed obvious differences toward the main diagnostic
traits, such as alternate bearing phenomena, the ratio of kernel weight to total
nut weight, split nuts percentage and loss percentage. Genotype V4 was
recognized by some important characters compared with the other genotypes
studied. Cluster analysis was assessed according to the existence or absence of
the most important studying morphological and agronomic indexes. All
genotypes studied and comparative cultivars were clustered into four groups.
RAPD technique was achieved using 25 randomly primers, 19 of which were
polymorphic with an average of polymorphism percentage of (66.47%). The
highest value of genetic similarity (0.81) was between Ashoury and V5
genotype, whereas the lowest value (0.56) was between V3 and V6. Cluster
analysis depending on RAPD data divided the population studied into two main
groups. RAPD unique band was estimated (37 unique band, 21 positive and 16
negative).
It was concluded that RAPD technique can be used efficiently to emphasize,
identify, insure and evaluate the genetic diversity of Pistachio vera.
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