Random Amplified Polymorphic DNA (RAPD) was used to evaluate genetic
diversity among 11 Yemeny pomegranate types. Samples were collected in 2004
from three main regions: east, middle and north of the country (Rada, Hada
and Saada). PCR amplificati
on of pomogranate DNA with 30 random primers
generated 1013 bands, 118 of them were polymorphic 11.5%. Results showed
that the genetic diversity within the tested types was limited. Similarity values
ranged between 59 –96 % with a mean of 79 %. Statistical analysis showed
that the types can be divided into two clusters: cluster 1 with Orkobi and
Mellies types found in the Hada region; cluster 2 includes nine types belonging
to the Saada and Radaa regions .High similarity (90–96%) was obtained with
Khazemy, Liessy 1, Liessy 2, Taefi, Ahmer and Balady types. Whereas, some
types could be considerd as separate cultivars due to their high genetic
diversity such as, Orkoby and Mellies (Hada region) in addition to Bohssom,
Sommaty and Liessy 3 in the Saada region.
This research was carried out in molecular biology laboratory
faculty of agriculture /Damascus University during the agriculture
season 2015-2016, to study the genetic diversity and determine the
degree of genetic similarity among eleven 11 cultiv
ated vetch
(Aleppo, Idleb, Kamishly, Hassekeh, Tartous, Lattakia, Homs,
Hama, Sweida, Damascus,Daraa) by using the technique ISSR.
