Ephedra is a dioecious, drought- and frost-resistant, perennial, evergreen shrub
with high medicinal value due to its content of ephedrine alkaloids. It became quite clear throughout
referential studies that this significant medical plant which is spontaneously widespread in Syria and there
have been an increased international demand on it hasn’t been studied yet.
The objective of this study was to characterize and determine the genetic
variation among twelve cultivars and four rootstocks, belonging to Amygdalus
genus in Syria using the simple sequence repeats (SSRs) marker. It was found
that 154 alleles we
re identified by using 26 primer pairs, and the proportion of
the specific ones was 30.52%. In addition, it was revealed that all the studied
SSR loci produced polymorphic alleles. The average of PIC, He and Ho were
0.58, 0.61, 0.31, respectively.
This investigation was carried out at the Laboratory of Biotechnology at
General Commission for Scientific Agricultural Research (GCSAR), during the
season 2010-2011. The aim of this research was to study the genetic diversity
among twenty individ
ual plants of seven species and to determine the degree of
genetic similarity using the technique ISSR (Inter Simple Sequence Repeats)
and 10 primers were used for this purpose. All primers proved their
effectiveness in showing polymorphism between the studied species, primers
gave a total 195 allele with a polymorphic percentage 100%. The number of
bands for each primer varied from a minimum of 12 bands for the primer
(ISSR-4) to a maximum of 27 bands for the primer (ISSR-862) in an average of
19.5 bands for each primer, cluster analysis and Dendrogram showed the
highest degree of genetic similarity between accession A.leucoclada1 and
A.leucoclada2 (0.64), while it was low between species A.leucoclada3 and
A.glauca2 (0.10). Results showed vast genetic diversity among the studied
species.
This research was carried out in the laboratories of the Atomic
Energy Commission Molecular Biology and Biotechnology
Department in Damascus to study the molecular characterization of
five of local and introduced grapes varieties which collected f
rom
the Pome and Grapevine Research Center in Sweida using 20
primer pairs of SSR.
تتميز طرز البندورة المحلية بالعديد من الصفات المهمة بالمقارنة مع الهجن المدخلة وتعد مخزوناً وراثياً قيماً يجب المحافظة عليه
