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Register a new userMolecular Characterization of Some Syrian Vetch Genotypes (Vicia Sativa) Using ISSR
التوصيف الجزيئي لبعض الطرز الوراثية من البيقية المزروعة Vicia Sativa المنتشرة في سورية باستخدام تقانة ISSR
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أجريت الدراسة على إحدى عشرة طرازا وراثياً من البيقية المزروعة تم جمعها من مواقع جغرافية مختلفة في سورية (حلب، إدلب، القامشلي، الحسكة، طرطوس، اللاذقية، حمص، حماة، السويداء، دمشق، درعا) و نُفذ البحث في مخبر التقانات الحيوية في كلية الزراعة بجامعة دمشق بغية تحديد درجة القرابة الوراثية بين الطرز المدروسة باستخدام تقنية ISSR.
This research was carried out in molecular biology laboratory faculty of agriculture /Damascus University during the agriculture season 2015-2016, to study the genetic diversity and determine the degree of genetic similarity among eleven 11 cultivated vetch (Aleppo, Idleb, Kamishly, Hassekeh, Tartous, Lattakia, Homs, Hama, Sweida, Damascus,Daraa) by using the technique ISSR.
References used
ADONINA, I. G; E.A.SALINA; E.G.PESTOVA AND M.S.RÖDER. 2005. Transferability of Wheat Microsatellites to Diploid Aegilops Species and Determination of Chromosomal Localizations of Microsatellites in the S Genome. 48:959-970
AMMIRAJU, J.S.S., DHOLAKIA, B.B., SANTRA, D.K., SINGH, H., LAGU, M.D., TAMHANKAR, S.A., DHALIWAL, H.S., RAO, V.S., GUPTA, V.S. AND RANJEKAR, P.K 2001. Identification of inter simple sequece repeat (ISSR) markers associated withseed size in wheat. Theor. Appl. Genet. 102: 726-732
BHAGYAWANT, S. SRIVASTAVA, N. 2008. Genetic fingerprinting of chickpea (Cicer arietinum L.) germplasm using ISSR markers and their relationships. African Journal of Biotechnology Vol. 7 (24): 4428-4431
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The research was executed in Biotechnology Lab (Faculty of
Agriculture – Damascus University), during the season 2016.
Sixteen genotypes of Sunflower Oil were planted to study the
genetic diversity among these genotypes and to determine the
degree of genetic Agreement using the technique SSR (Simple
Sequence Repeats).
Seven plant samples were collected from some locations of Syrian juniper
(Juniperus drupacea Labill.) in Syria for molecular characterization and to
determine the genetic relationships between them using ISSR technique (Inter
Simple Sequence Repea
ts). Twenty three ISSR primers were used for this
purpose, twelve primers showed polymorphism between studied samples and
gave 89 bands, with polymorphism percentage of 95.5%. The band number
resulted from each primer ranged between 4 bands for primers ISSR5 and
ISSR9, and 12 bands for the primer ISSR1, with an average of 7.42 bands per
primer. The minimum polymorphic percentage was 25% for primer ISSR9,
and the maximum polymorphic percentage was 100% for the all primers
except the primer ISSR9. The study showed correlation between the genetically
converged samples and the collection sites (geographic correlation), the highest
genetic relationship (93%) was within Latakia samples (Komat Alnabi Yonis –
Jobet Bergal) and the lowest genetic relationship (42%) was between samples
from Hamah (Jeb Alahmer) and Latakia (Almakamat) which refers to high
genetic variation. The cluster analysis showed that the samples from nearby
locations were gathered.
Morphological and molecular characterization for six seedling genotypes of
Pistachio vera L (V1,V2,V3,V4,V5, and V6) in comparison with the most
commercial and wide spread varieties, Ashoury andWhite batoury was carried
out in Swaida province, sou
thern of Syria. Nuts chemical contents (dry
substance, moisture, total sugar and oil content) for both fresh and dry nuts
weight were also evaluated.
Seedling genotypes showed obvious differences toward the main diagnostic
traits, such as alternate bearing phenomena, the ratio of kernel weight to total
nut weight, split nuts percentage and loss percentage. Genotype V4 was
recognized by some important characters compared with the other genotypes
studied. Cluster analysis was assessed according to the existence or absence of
the most important studying morphological and agronomic indexes. All
genotypes studied and comparative cultivars were clustered into four groups.
RAPD technique was achieved using 25 randomly primers, 19 of which were
polymorphic with an average of polymorphism percentage of (66.47%). The
highest value of genetic similarity (0.81) was between Ashoury and V5
genotype, whereas the lowest value (0.56) was between V3 and V6. Cluster
analysis depending on RAPD data divided the population studied into two main
groups. RAPD unique band was estimated (37 unique band, 21 positive and 16
negative).
It was concluded that RAPD technique can be used efficiently to emphasize,
identify, insure and evaluate the genetic diversity of Pistachio vera.
This investigation was carried out at the Laboratory of Biotechnology at
General Commission for Scientific Agricultural Research (GCSAR), during the
season 2010-2011. The aim of this research was to study the genetic diversity
among twenty individ
ual plants of seven species and to determine the degree of
genetic similarity using the technique ISSR (Inter Simple Sequence Repeats)
and 10 primers were used for this purpose. All primers proved their
effectiveness in showing polymorphism between the studied species, primers
gave a total 195 allele with a polymorphic percentage 100%. The number of
bands for each primer varied from a minimum of 12 bands for the primer
(ISSR-4) to a maximum of 27 bands for the primer (ISSR-862) in an average of
19.5 bands for each primer, cluster analysis and Dendrogram showed the
highest degree of genetic similarity between accession A.leucoclada1 and
A.leucoclada2 (0.64), while it was low between species A.leucoclada3 and
A.glauca2 (0.10). Results showed vast genetic diversity among the studied
species.
The research was conducted in Al-Bassa village, Lattakia Governorate/Syria,
during the agricultural seasons 2014/2015 and 2015/2016, in order to study the
most important economic traits of the selected plants from plant population of
local vetch,
which seeds was obtained from farmers who keep their local seeds and
grow it season after another. The analysis of variance results (ANOVA) showed
significant differences between the selected plants (200 plants) in all studied
traits, which shows the importance and effectiveness of the individual selection
to get high degree of homogeneity plants, which have many desired phenological,
morphological, physiological and productivity characteristics of local vetch. Taking
into consideration that seed productivity was adopted as a determining factor for
the election. The study also showed the possibility of increasing the productivity of
the local vetch populations through the use of superior plants selection (plants No.
9, 21, 26, 47, 52, 78, 105, 118, and 199), and the possibility of doubling production
through the use of promising plants which were selected (plants No. 9, 26, 47, and
199), and use them in subsequent breeding programs, as they represent an important
genetic tank of many desirable traits.
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