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Register a new userMolecular Characterization of Juniperus drupacea Labill. (Syrian juniper) population in Syria Using ISSR Technique
التوصيف الجزيئي لطرز من العدريش Syrian juniper) Juniperus drupacea Labill) في سورية باستخدام مؤشرات الـ ISSR
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Damascus University ورقة بحثية
Publication date
2014
fields
Pharmacy
and research's language is
العربية
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Shamra Editor
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جمِعت 7 عينات نباتية من عدة مواقع لانتشار نبات العدريش .Labill drupacea Juniperus في سورية بهدف توصيفها جزيئياً و تحديد درجة القرابة الوراثيـة فيمـا بينهـا، باسـتخدام تقنيـة ISSR (Repeats Sequence Simple Inter) . و قد استخدم لهذا الغرض 23 بادئةً، أظهرت 12 بادئةً منهـا قدرتها على كشف تباينات و اختلافات بين العينات المدروسة، و نتج عن اسـتخدامها 89 حزمـة. و بلغـت نسبة هذه التعددية 5.95 % و تراوح عـدد الحـزم المكـاثرة لكـل بادئـة بـين 4 أربـع حـزم مـع البادئتين (ISSR9, ISSR5) و 12 حزمة مع البادئة (ISSR1) بمتوسط قدره 42.7 حزمةً/بادئة. و كانت النسبة المئوية للتعددية الشكلية الأقل مع البادئة (ISSR9) بمقـدار 25 % و الأكبـر مـع كـل البادئـات باستثناء (ISSR9) بمقدار 100% و بينت الدراسة ارتباط العينات المتقاربة وراثياً بالمناطق التي جمعـت منها (الموقع الجغرافي)، فقد كانت أعلى درجة قرابة وراثية (93) % بين عينات اللاذقيـة (قمـة النبـي يونس) و (جوبة برغال)، و أقل درجة قرابة وراثية (42) % بين عينات حمـاه (جـب الأحمـر) و اللاذقيـة (المقامات). و هذا يدل على وجود بعد وراثي عالٍ بينها. كما أن التحليل العنقودي فرز العينات التي جمعت من مناطق متقاربة جغرافياُ.
Seven plant samples were collected from some locations of Syrian juniper (Juniperus drupacea Labill.) in Syria for molecular characterization and to determine the genetic relationships between them using ISSR technique (Inter Simple Sequence Repeats). Twenty three ISSR primers were used for this purpose, twelve primers showed polymorphism between studied samples and gave 89 bands, with polymorphism percentage of 95.5%. The band number resulted from each primer ranged between 4 bands for primers ISSR5 and ISSR9, and 12 bands for the primer ISSR1, with an average of 7.42 bands per primer. The minimum polymorphic percentage was 25% for primer ISSR9, and the maximum polymorphic percentage was 100% for the all primers except the primer ISSR9. The study showed correlation between the genetically converged samples and the collection sites (geographic correlation), the highest genetic relationship (93%) was within Latakia samples (Komat Alnabi Yonis – Jobet Bergal) and the lowest genetic relationship (42%) was between samples from Hamah (Jeb Alahmer) and Latakia (Almakamat) which refers to high genetic variation. The cluster analysis showed that the samples from nearby locations were gathered.
References used
Adams, R. and T. Demeke. 1993. Systematic relationships in Juniperus based on DNAs (RAPDs). Taxon, 42(3): 553-571
Adams, R., A. Schwarzbach and R. Pandey. 2003. The concordance of terpenoid, ISSR and RAPD markers and ITS sequence data sets among genotypes: an example from Juniperus, Biochemical Systematics and Ecology, 31: 375–387
Bornet, B., F. Goraguer, G. Joly and M. Branchard. 2002. Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs), Genome, 45: 481-484
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This investigation was carried out at the Laboratory of Biotechnology at
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genetic similarity using the technique ISSR (Inter Simple Sequence Repeats)
and 10 primers were used for this purpose. All primers proved their
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referential studies that this significant medical plant which is spontaneously widespread in Syria and there
have been an increased international demand on it hasn’t been studied yet.
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