جمِعت 7 عينات نباتية من عدة مواقع لانتشار نبات العدريش .Labill drupacea Juniperus في
سورية بهدف توصيفها جزيئياً و تحديد درجة القرابة الوراثيـة فيمـا بينهـا، باسـتخدام تقنيـة ISSR
(Repeats Sequence Simple Inter) . و قد استخدم لهذا الغرض 23 بادئةً، أظهرت 12 بادئةً منهـا
قدرتها على كشف تباينات و اختلافات بين العينات المدروسة، و نتج عن اسـتخدامها 89 حزمـة. و بلغـت
نسبة هذه التعددية 5.95 % و تراوح عـدد الحـزم المكـاثرة لكـل بادئـة بـين 4 أربـع حـزم مـع
البادئتين (ISSR9, ISSR5) و 12 حزمة مع البادئة (ISSR1) بمتوسط قدره 42.7 حزمةً/بادئة. و كانت
النسبة المئوية للتعددية الشكلية الأقل مع البادئة (ISSR9) بمقـدار 25 % و الأكبـر مـع كـل البادئـات
باستثناء (ISSR9) بمقدار 100% و بينت الدراسة ارتباط العينات المتقاربة وراثياً بالمناطق التي جمعـت
منها (الموقع الجغرافي)، فقد كانت أعلى درجة قرابة وراثية (93) % بين عينات اللاذقيـة (قمـة النبـي
يونس) و (جوبة برغال)، و أقل درجة قرابة وراثية (42) % بين عينات حمـاه (جـب الأحمـر) و اللاذقيـة
(المقامات). و هذا يدل على وجود بعد وراثي عالٍ بينها. كما أن التحليل العنقودي فرز العينات التي جمعت
من مناطق متقاربة جغرافياُ.
Seven plant samples were collected from some locations of Syrian juniper
(Juniperus drupacea Labill.) in Syria for molecular characterization and to
determine the genetic relationships between them using ISSR technique (Inter
Simple Sequence Repeats). Twenty three ISSR primers were used for this
purpose, twelve primers showed polymorphism between studied samples and
gave 89 bands, with polymorphism percentage of 95.5%. The band number
resulted from each primer ranged between 4 bands for primers ISSR5 and
ISSR9, and 12 bands for the primer ISSR1, with an average of 7.42 bands per
primer. The minimum polymorphic percentage was 25% for primer ISSR9,
and the maximum polymorphic percentage was 100% for the all primers
except the primer ISSR9. The study showed correlation between the genetically
converged samples and the collection sites (geographic correlation), the highest
genetic relationship (93%) was within Latakia samples (Komat Alnabi Yonis –
Jobet Bergal) and the lowest genetic relationship (42%) was between samples
from Hamah (Jeb Alahmer) and Latakia (Almakamat) which refers to high
genetic variation. The cluster analysis showed that the samples from nearby
locations were gathered.
References used
Adams, R. and T. Demeke. 1993. Systematic relationships in Juniperus based on DNAs (RAPDs). Taxon, 42(3): 553-571
Adams, R., A. Schwarzbach and R. Pandey. 2003. The concordance of terpenoid, ISSR and RAPD markers and ITS sequence data sets among genotypes: an example from Juniperus, Biochemical Systematics and Ecology, 31: 375–387
Bornet, B., F. Goraguer, G. Joly and M. Branchard. 2002. Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs), Genome, 45: 481-484
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