Winter chilling requirements are critical for flowering and fruiting of many
fruit species, with evidence that pistachio has been adversely affected by warm
winter, so that estimating chilling and heat requirements is crucial for
identifying appro
priate cultivars for given site. In our study, different levels of
chilling hours were applied (500-600-700-800-900-1000-1100-1200-1300-1400 h).
The objective of this study was to characterize and determine the genetic
variation among twelve cultivars and four rootstocks, belonging to Amygdalus
genus in Syria using the simple sequence repeats (SSRs) marker. It was found
that 154 alleles we
re identified by using 26 primer pairs, and the proportion of
the specific ones was 30.52%. In addition, it was revealed that all the studied
SSR loci produced polymorphic alleles. The average of PIC, He and Ho were
0.58, 0.61, 0.31, respectively.
The experiment detected the chill hour requirements of 6 female and 8 male
varieties of Pistachio (Pictacia vera L.) cultivated in Aleppo, Idleb, Hama and
Dar’a in Syria needed to break down their bud dormancy. Studied cuttings left
at 2°C in cool
chamber for 650, 750, 850, 950, 1050 and 1150 hours were placed
into growth chamber offering temperature, moisture and brightness similar to
those available in nature during the period of blooming flowers. Statistical
analysis distinguished clearly between the chill requirements of the female and
the male varieties, where the males needed lower cold hours. Also, the female
varieties showed differences among them in their chill requirements due to
genetic resources which were affected by their geographical areas and
attitudes. The period in the growth chamber needed to reach a proper
blooming ranged between 32 days in the case of Ashoury variety cultivated in
Dar’a with 1150 chill hours and 54 days with Nab Aljamal variety existed in
Hama and Idleb with 650 chill hours. Meanwhile, in the male varieties this
figure was between 22 days for male 2 cultivated in Idleb (1150 chill hour) and
43 days for Male E in Dar’a (650 chill hours). The cluster configuration
between the males and females varieties was sorted by putting them in two
relative distant sub-clusters. The females varieties were distributed sorted into
sub-groups as well, insuring the similarity among the ones belong. In addition,
The males varieties were distributed into two types depending on their earliness
blooming time (early: Hama1, Hama2, Idleb1, Idleb2 – late: A jellin, E Jellin,
Aleppo1, Aleppo2). The results could be useful for the decisions of what
varieties to be grown in certain places depending on natural chill hours
available there.
Morphological and molecular characterization for six seedling genotypes of
Pistachio vera L (V1,V2,V3,V4,V5, and V6) in comparison with the most
commercial and wide spread varieties, Ashoury andWhite batoury was carried
out in Swaida province, sou
thern of Syria. Nuts chemical contents (dry
substance, moisture, total sugar and oil content) for both fresh and dry nuts
weight were also evaluated.
Seedling genotypes showed obvious differences toward the main diagnostic
traits, such as alternate bearing phenomena, the ratio of kernel weight to total
nut weight, split nuts percentage and loss percentage. Genotype V4 was
recognized by some important characters compared with the other genotypes
studied. Cluster analysis was assessed according to the existence or absence of
the most important studying morphological and agronomic indexes. All
genotypes studied and comparative cultivars were clustered into four groups.
RAPD technique was achieved using 25 randomly primers, 19 of which were
polymorphic with an average of polymorphism percentage of (66.47%). The
highest value of genetic similarity (0.81) was between Ashoury and V5
genotype, whereas the lowest value (0.56) was between V3 and V6. Cluster
analysis depending on RAPD data divided the population studied into two main
groups. RAPD unique band was estimated (37 unique band, 21 positive and 16
negative).
It was concluded that RAPD technique can be used efficiently to emphasize,
identify, insure and evaluate the genetic diversity of Pistachio vera.
This research was conducted during the 2009-2010 seasons in order to
inventory and locate the spread of wild olive in 17 locations in the province of
Hama. GIS and digital maps were used to characterize the sites of wild live
distribution geo- gra
phically, topography and the dominant vegetation. Results
showed highly environmental flexibility of Olive wild toward the sea level rise.
It was observed prevalence at high 185 m of sea level in Naora Shatha and
grading up to 994 m in the western side of Abu Qubeis protected area and more
prevalence at the wet and top wet bioatmosphere. Chemical and physical
analyses for soils of locations showed dominancy of the mud structure in most
locations which reach in some of them to 74% and this help in retaining soil
moisture. The vegetation prevalent in locations showed spreading of Oak trees
in addition to morphological varieties of wild Olives the sites studied with
different forms of degradation in most or all locations.
The chill hour requirements of female and male trees of Pistacia atlantica,
Pistacia palaestina and Pistacia khinjuk have been examined. This was carried
out through putting the studied cuttings in cool chamber for different perriods
of temperatur
es of 2 °C (550, 650, 750, 850, 950, and 1050 hours). The cuttings,
later on, were moved into growth chamber which offers temperature, moisture
and light similar to that available at the time of flowering season naturally.
Thirteen phenotypes of cultivated wild olive were selected during 2007- 2008
from four orchards in Mesiaf region, to study oil percentage and fatty acids
contents in these types for choosing types for oil production, in order to
propagate and cons
erve them in a germplasm, and then to be cultivated
because they have adapted to local climate in this region. The results were
analyzed statistically by using Gen-State program to calculate least significant
differences between values. The results confirmed the high biodiversity in these
studied types, and they were classified into five groups according to their oil
percentage and contents of oleic acid. The oil percentage ranged from 3.9 to
27.3 %, and the fatty acids had significant differences between them, the oleic
acid was in olive oil between 56.7 and 75.5%. The types P1-1 and p1-3 showed
the best results for producing oil.
Random Amplified Polymorphic DNA (RAPD) was used to evaluate genetic
diversity among 11 Yemeny pomegranate types. Samples were collected in 2004
from three main regions: east, middle and north of the country (Rada, Hada
and Saada). PCR amplificati
on of pomogranate DNA with 30 random primers
generated 1013 bands, 118 of them were polymorphic 11.5%. Results showed
that the genetic diversity within the tested types was limited. Similarity values
ranged between 59 –96 % with a mean of 79 %. Statistical analysis showed
that the types can be divided into two clusters: cluster 1 with Orkobi and
Mellies types found in the Hada region; cluster 2 includes nine types belonging
to the Saada and Radaa regions .High similarity (90–96%) was obtained with
Khazemy, Liessy 1, Liessy 2, Taefi, Ahmer and Balady types. Whereas, some
types could be considerd as separate cultivars due to their high genetic
diversity such as, Orkoby and Mellies (Hada region) in addition to Bohssom,
Sommaty and Liessy 3 in the Saada region.
Semi-wooden cuttings of two - jujuba cultivars were vegetitirely propagated
under greenhouse conditions, by using different concentrations of Indole
butyric acid (IBA), at Hanadi Research Station, Lattakia.
The rooting percentage reached 71.3% aft
er two months for the Ziziphus
jujube mill, and 83.3% for the Ziziphus jujube tayan tsizao, when they were
treated with 4000 mg/L of (IBA) for five seconds while the rooting percentage
was zero for the non-treated cuttings (Control) .
Study was conducted in five locations as the important areas for
pomegranate cultivation and production in Yemen, during the period (15/1–
15/8/2004). Eight accessions of local pomegranate were used in this study:
Taefi, Orkobi, Mellies–Hada, Khaz
emy, Sommaty, Kodary, Chiny and Mellies–
Raudha in addition to the wild pomegranate type (Socotria). The
morphological characters were determined for leaves, flowers,fruits and seeds.
The chemical analysis for juice was also assessed. Maximum rate for fruit
weight was 527.3 gm for Kodary accession, whereas fruit Juic varied between
49.5–63.2%, while peel / fruit was varied between15% in Taefi and 35.9 % in
Chiny, whereas the peel thickness varied between 1.5–4.2 mm. The Scotria was
found to be evergreen, and flowers around the year. Kazemy accession gave
higher fruits number/tree with rate 246.7. The greatest rate of T.S.S and
Vitamin C were 18.57% and 11.12 mg respectively for Chiny accession, and the
highest content of organic acids in these accessions was 0.54 % for Taefi.
