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Register a new userMolecular characterization of some almond cultivars and rootstocks in Syria using SSR markers
التوصيف الجزيئي لبعض أصناف و أصول اللوز في سورية باستخدام المؤشرات الـ SSRs
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Damascus University ورقة بحثية
Publication date
2014
fields
Groves
and research's language is
العربية
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هدف البحث إلى توصيف التباينات الوراثية و تحديدها بين اثني عشر صنفاً و أربعة أصول وراثية تتبع لجنس اللوز Amygdalus المنتشرة في سورية باستخدام تقنية المقاطع البـسيطة المتكـررة (SSRs)، استخدم 26 زوجا من البادئات و حدد 154 قرينا، و بلغت نـسبة القـرائن (alleles) المتخصـصة منهـا 52.30 ،% و تبين أن جميع المواقع الدقيقة المدروسة أظهرت تباينات بالقرائن التي كشفت ما بين الطـرز المدروسة، بلغ متوسط قيم كل من معامل PIC و الـ He و الـ Ho 58.0 و 61.0 و 31.0 ،على التوالي.
The objective of this study was to characterize and determine the genetic variation among twelve cultivars and four rootstocks, belonging to Amygdalus genus in Syria using the simple sequence repeats (SSRs) marker. It was found that 154 alleles were identified by using 26 primer pairs, and the proportion of the specific ones was 30.52%. In addition, it was revealed that all the studied SSR loci produced polymorphic alleles. The average of PIC, He and Ho were 0.58, 0.61, 0.31, respectively.
References used
Amirbakhtiar, N., B. H. Shiran. H. Moradi and B.E. Sayed-Tabatabaei. 2006. Molecular characterization of almond cultivars using microsatellite markers, ISHS. Acta. Horticulture. 726 :4
Aranzana, M. J, J. Garcia-Mas. J. Carbo and P. Aru`s. 2002. Development and variability analysis of microsatellite markers in peach, Plant Breed. 121(1):87-92
Botstein, D., L. Raymond and L. White.1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms, Am. J. Hum. Genet. 32: 314-331
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