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Register a new userStudy of the Genetic Diversity of Some Pomegranate Types in Yemen using RAPD Analysis
دراسة التنوع الوراثي لبعض طرز الرمان في اليمن باستخدام تحاليل الـ RAPD
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استخدمت تقانة الدنا المضخم عشوائياً (RAPD) لدراسة التنوع الوراثي لبعض طرز الرمان التـي بلغ عددها 11 طرازاً وراثياً جمعت من 3 مناطق رئيسية في شـرق الـيمن و وسـطه و شـماله (رداع، الحداء، صعدة) خلال العام 2004 م. تمت عمليه مكاثرة قطع الDNA و تضخيمها خلال التفاعل التسلسلي للبوليميراز (PCR) باسـتخدام 30 بادئة عشوائية أنتجت 1013 حزمة (شدفة) منها 118 أعطت تعددية شكلية 5.11 % و قد أوضحت النتائج أن الاختلاف الوراثي بين الطرز المستخدمة كان محدوداً حيث تراوحت درجه القرابة بينهـا مـن % 79 قدره بمتوسط % 96 -59 أظهر التحليل الإحصائي إمكانية تقسيم الطرز المدروسة إلى مجموعتين: الأولـى تضـم الطـرازين عرقبي و مليس في منطقه الحداء، أما المجموعة الثانية فتضم 9 طرز تنتمي إلى منـاطق صـعدة و رداع و الحداء. وجدت درجة عالية من القرابة الوراثية (90 – 96 %) بين طرز الرمـان خازمي، ليسي 1 ،ليسـي 2 ،طائفي، أحمر، بلدي في حين عدتْ بعض الطرز أصنافاً مستقلة نظراً لارتفاع درجه الاختلاف الوراثي بينها مثل طرازي الرمان عرقبي و مليس في منطقة الحداء بالإضافة إلى الطرز بحصم، صماطي، ليسـي 3 في منطقه صعدة.
Random Amplified Polymorphic DNA (RAPD) was used to evaluate genetic diversity among 11 Yemeny pomegranate types. Samples were collected in 2004 from three main regions: east, middle and north of the country (Rada, Hada and Saada). PCR amplification of pomogranate DNA with 30 random primers generated 1013 bands, 118 of them were polymorphic 11.5%. Results showed that the genetic diversity within the tested types was limited. Similarity values ranged between 59 –96 % with a mean of 79 %. Statistical analysis showed that the types can be divided into two clusters: cluster 1 with Orkobi and Mellies types found in the Hada region; cluster 2 includes nine types belonging to the Saada and Radaa regions .High similarity (90–96%) was obtained with Khazemy, Liessy 1, Liessy 2, Taefi, Ahmer and Balady types. Whereas, some types could be considerd as separate cultivars due to their high genetic diversity such as, Orkoby and Mellies (Hada region) in addition to Bohssom, Sommaty and Liessy 3 in the Saada region.
References used
Anonymous. (2000). Arab Organization for Agricultural Development, Khartoom - December, 20:115
Baranski, R., Grzebelus, D. and Frese, L. (2001). Evaluation of genetic diversity in a Garden Beet germplasm collection using RAPD technique. Acta Hort. (ISHS). 546, 165-169
EL Kassas, S. E., Amen, K. I. A., Hussein, A. A. and Osrnan, S. M. (1992). Effects of certain methods of weed control and nitrogen fertilization on the yield, fruit quality and some nutrient contents of Manfalouty pomegranate trees: 1-Flowering and fruit setting. Assist Jounal of Agricultural Sciences. 23 (3), 183-198
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يعد الماعز الشامي في سورية من الحيوانات الزراعية المحلية الهامة لما يملكه من مزايا ومواصفات تمكنه من الإنتاج والتناسل تحت الظروف البيئية القاسية لهذا فقد استخدم في برامج الخلط التربوي مع الماعز الجبلي كمعطي لمورثات صفة إنتاج الحليب الجيدة للحيوانات الخليطة
The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorp
hic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
Shami goat in Syria is one of the important domestic breeds, because of its
high productivity and breeding capacity under the hard environmental
conditions and it is still being used in crossbreeding with Mountain goats as a
donor for milk produci
ng traits. This breed is still far away from scientific
investigations even it has been exposed to a random crossbreeding with
Mountain goats by goat breeders. This has lead to the possibility of losing some
of its genetic characteristics which are not detected yet. In this research the
genetic diversity among groups of pure Shami goats was determined using
ISSR-PCR on blood and results indicated that there was a molecular genetic
diversity among studied goats and polymorphic rate was register to 100%.
Randomly amplified polymorphic DNA (RAPD) technique was used to study the genetic relationship among 10 wild accessions of Origanum syriacum grown in Syria.
The results of this study may have a great impact on the
national biodiversity program whic
h lacks the employment of
molecular techniques, and thus we recommend making use
of these techniques in genotype identification in general, and
studying the relationship among them, and in particular in
studying important wild species of medical importance which
have not been described yet on the molecular level.
Nine Syrian cotton genotypes i.e. Aleppo 33/1, Aleppo 118, Aleppo 90, Aleppo 40,
Aleppo 124 Rakka5, Deir Al-Zour 22, Line 106 and Rusafa, were used for statistical
and genetic analysis for productivity and some chemical indicators of seeds:
Lint p
ercentage%, cotton weight per plant/g, percent of oil in seeds and pulp, percent
of protein in seeds and pulp, percent of humidity in seeds and pulp, to explore
the potentiality of the genotypes in the studied region, and to establish a program
for the production of cotton and seeds, also to determine the selective indecies to be
used to improve cotton productivity and seed components, using randomized complete
block design with three replications. The experiment was conducted in Salhab
village, Al-Ghab region, Hama governorate, Syria, during 2015 season.
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