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Morphological and molecular characterization for some seeding of Pistachio vera genotypes and cultivars in Swaida province

التوصيف الشكلي و الجزيئي لبعض الطرز الوراثية للفستق الحلبي vera.P في محافظة السويداء

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 Publication date 2012
and research's language is العربية
 Created by Shamra Editor




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Morphological and molecular characterization for six seedling genotypes of Pistachio vera L (V1,V2,V3,V4,V5, and V6) in comparison with the most commercial and wide spread varieties, Ashoury andWhite batoury was carried out in Swaida province, southern of Syria. Nuts chemical contents (dry substance, moisture, total sugar and oil content) for both fresh and dry nuts weight were also evaluated. Seedling genotypes showed obvious differences toward the main diagnostic traits, such as alternate bearing phenomena, the ratio of kernel weight to total nut weight, split nuts percentage and loss percentage. Genotype V4 was recognized by some important characters compared with the other genotypes studied. Cluster analysis was assessed according to the existence or absence of the most important studying morphological and agronomic indexes. All genotypes studied and comparative cultivars were clustered into four groups. RAPD technique was achieved using 25 randomly primers, 19 of which were polymorphic with an average of polymorphism percentage of (66.47%). The highest value of genetic similarity (0.81) was between Ashoury and V5 genotype, whereas the lowest value (0.56) was between V3 and V6. Cluster analysis depending on RAPD data divided the population studied into two main groups. RAPD unique band was estimated (37 unique band, 21 positive and 16 negative). It was concluded that RAPD technique can be used efficiently to emphasize, identify, insure and evaluate the genetic diversity of Pistachio vera.


Artificial intelligence review:
Research summary
يتناول البحث توصيفاً مورفولوجياً وجزيئياً باستخدام تقنية RAPD لستة طرز بذرية من الفستق الحلبي (V1, V2, V3, V4, V5, V6) في محافظة السويداء، جنوب سوريا، بالمقارنة مع أصناف عاشوري وباتوري أبيض. تم تحليل المحتوى الكيميائي للثمار من حيث المادة الجافة، الرطوبة، السكريات الكلية، ومحتوى الزيت. أظهرت الطرز اختلافات واضحة في الصفات المورفولوجية والزراعية مثل ظاهرة تبادل الحمل، نسبة تصافي النواة، نسبة الثمار المتشققة، ومعدل الفقد. تميز الطراز V4 بالعديد من المواصفات المهمة مقارنةً بالطرز الأخرى. استخدمت تقنية RAPD مع 25 بادئاً عشوائياً، حيث أظهر 19 منها تعددية شكلية بنسبة 66.47%. بلغت أعلى درجة للتشابه الوراثي (0.81) بين عاشوري والطراز V5، وأقلها (0.56) بين الطرازين V3 وV6. أظهرت النتائج أن تقنية RAPD فعالة في دراسة التباين الوراثي وتعريف المصادر الوراثية وتوثيقها ضمن النوع P.vera. توصي الدراسة بتوثيق الطراز V4 باستخدام طرق جزيئية أكثر دقة ودراسة سلوكيته في مواقع بيئية مختلفة.
Critical review
دراسة نقدية: يعتبر البحث شاملاً ومفصلاً في توصيف الطرز الوراثية للفستق الحلبي من الناحية المورفولوجية والجزيئية. ومع ذلك، يمكن توجيه بعض الملاحظات النقدية لتحسين الدراسة. أولاً، قد يكون من المفيد تضمين عدد أكبر من الطرز الوراثية والأصناف المحلية والدولية لزيادة شمولية الدراسة. ثانياً، يمكن تعزيز التحليل الإحصائي باستخدام تقنيات تحليل بيانات أكثر تقدماً لضمان دقة النتائج. ثالثاً، يمكن أن تكون الدراسة أكثر شمولية إذا تم تضمين تأثير العوامل البيئية المختلفة على الصفات المورفولوجية والجزيئية للطرز المدروسة. وأخيراً، قد يكون من المفيد إجراء دراسات مستقبلية لتقييم الأداء الزراعي والاقتصادي للطرز المدروسة في ظروف زراعية متنوعة.
Questions related to the research
  1. ما هي التقنية المستخدمة في التوصيف الجزيئي للطرز الوراثية في الدراسة؟

    تم استخدام تقنية RAPD (التضخيم العشوائي للحمض النووي) في التوصيف الجزيئي للطرز الوراثية في الدراسة.

  2. ما هي الطرز الوراثية التي أظهرت أعلى درجة تشابه وراثي؟

    أظهرت الطرز الوراثية عاشوري والطراز V5 أعلى درجة تشابه وراثي بنسبة (0.81).

  3. ما هي الصفات المميزة للطراز V4 مقارنةً بالطرز الأخرى؟

    تميز الطراز V4 بارتفاع محتوى الثمار من الزيت، وارتفاع نسبة الثمار المتشققة، وارتفاع نسبة التصافي مقارنةً بالطرز الأخرى.

  4. ما هي التوصيات التي قدمتها الدراسة بشأن الطراز V4؟

    توصي الدراسة بتوثيق الطراز V4 باستخدام طرق جزيئية أكثر دقة ودراسة سلوكيته في مواقع بيئية مختلفة.


References used
Basha, A.I., S. Padulosi, K. Chabane, A. Hadj-Hassan, E. Dulloo, M. A. Pagnotta and E. Porceddu, 2007. Genetic Diversity of Syrian Pistachio (Pistacia vera L.) Varieties Evaluated by AFLP Markers. Genet. Rerso. Crop Evo. 54 (8): 1807-1816
Bardakci, F. 2001. Random amplified polymorphic DNA (RAPD) markers. Turk. J. Biol., 25: 185-196
Baghizadeh, A., Sh. Noroozi, and M. J. Avaran. 2010. Study on genetic diversity of some Iranian Pistachio (Pistacia vera L.) cultivars using random amplified polymorphic DNA (RAPD), Iiter sequence repeat (ISSR) and simple sequence repeat (SSR) markers: A comparative study. Afric. J. Biotechnol., 9(45): 7632-7640
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