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التوصيف الجزيئي لبعض الطرز الوراثية من البندورة المزروعة في سورية

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 Publication date 2016
and research's language is العربية
 Created by Shamra Editor




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References used
الصباغ عبد العزيز (2000) التصنيف النباتي جامعة دمشق , كلية الهندسة الزراعية
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This research was carried out in molecular biology laboratory faculty of agriculture /Damascus University during the agriculture season 2015-2016, to study the genetic diversity and determine the degree of genetic similarity among eleven 11 cultiv ated vetch (Aleppo, Idleb, Kamishly, Hassekeh, Tartous, Lattakia, Homs, Hama, Sweida, Damascus,Daraa) by using the technique ISSR.
Morphological and molecular characterization for six seedling genotypes of Pistachio vera L (V1,V2,V3,V4,V5, and V6) in comparison with the most commercial and wide spread varieties, Ashoury andWhite batoury was carried out in Swaida province, sou thern of Syria. Nuts chemical contents (dry substance, moisture, total sugar and oil content) for both fresh and dry nuts weight were also evaluated. Seedling genotypes showed obvious differences toward the main diagnostic traits, such as alternate bearing phenomena, the ratio of kernel weight to total nut weight, split nuts percentage and loss percentage. Genotype V4 was recognized by some important characters compared with the other genotypes studied. Cluster analysis was assessed according to the existence or absence of the most important studying morphological and agronomic indexes. All genotypes studied and comparative cultivars were clustered into four groups. RAPD technique was achieved using 25 randomly primers, 19 of which were polymorphic with an average of polymorphism percentage of (66.47%). The highest value of genetic similarity (0.81) was between Ashoury and V5 genotype, whereas the lowest value (0.56) was between V3 and V6. Cluster analysis depending on RAPD data divided the population studied into two main groups. RAPD unique band was estimated (37 unique band, 21 positive and 16 negative). It was concluded that RAPD technique can be used efficiently to emphasize, identify, insure and evaluate the genetic diversity of Pistachio vera.
The research was executed in Biotechnology Lab (Faculty of Agriculture – Damascus University), during the season 2016. Sixteen genotypes of Sunflower Oil were planted to study the genetic diversity among these genotypes and to determine the degree of genetic Agreement using the technique SSR (Simple Sequence Repeats).
This investigation was carried out at the Laboratory of Biotechnology at General Commission for Scientific Agricultural Research (GCSAR), during the season 2010-2011. The aim of this research was to study the genetic diversity among twenty individ ual plants of seven species and to determine the degree of genetic similarity using the technique ISSR (Inter Simple Sequence Repeats) and 10 primers were used for this purpose. All primers proved their effectiveness in showing polymorphism between the studied species, primers gave a total 195 allele with a polymorphic percentage 100%. The number of bands for each primer varied from a minimum of 12 bands for the primer (ISSR-4) to a maximum of 27 bands for the primer (ISSR-862) in an average of 19.5 bands for each primer, cluster analysis and Dendrogram showed the highest degree of genetic similarity between accession A.leucoclada1 and A.leucoclada2 (0.64), while it was low between species A.leucoclada3 and A.glauca2 (0.10). Results showed vast genetic diversity among the studied species.
This research was conducted to detect the morphological and molecular differences between golden henbane (Hyoscyamus aureus L) collected from south Syria. Seeds were collected from six locations, then sterilized and grown In Vitro. Grown plants we re described for some morphological characters. The molecular characterization was carried out using the method of Inter Simple Sequence Repeats (ISSR) to detect genetic variations. The plants showed significant (p<0.01) morphological differences among studied locations, Plant height ranged from 14.97 to 18.97 cm and stem color ranged from red to green. Trichome density also ranged between medium, high and very high. Furthermore, significant differences in leaves dimensions were observed according to the plant geographical location. The main root length ranged from 6.09 to 8.37 cm. Molecular analysis by ISSR produced a total of 56 bands, 49 of which were polymorphic (87.5%), resulted from using 11 non-specific primers. A genetic distance dendrogram was drawn. based on the results of genetic characterization of plant from different locations. The plants collected from Salkhad separated with a genetic distance of 0.312 while the plants collected from other locations were grouped together, at the same time, they were divided into two sub groups, the first group comprised plants collected from Qalamoun and Dimas regions with a genetic distance of 0.032, whereas sub group II content Bosra plants which separated clearly from those of Dara' dam and Wadi Al-Zaidi by a genetic distance of 0.017, These results may indicate a relationship between genetic characters of this plant species with geographic distribution.

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