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Register a new userThe Relationship Between Genetic Variation and GeographicalDistribution of Capparis SpinosaL. Phenotypes in Syria
العلاقة بين التنوع الوراثي والتوزع الجغرافي لطرز نبات القبار الشوكي Capparis spinosa L. في سورية
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Damascus University أطروحة دكتوراه
Publication date
2015
fields
Agricultural Engineering
and research's language is
العربية
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نفذ البحث خلال الفترة الواقعة بين 2010-2014 على عدد من عينات نباتات القبار الشوكي Capparis spinosa L. مجموعة من مناطق مختلفة من سورية بهدف توصيفها بيئياً وشكلياً ودراسة توزعها الجغرافي وتحليل بعض مضادات الأكسدة فيها ودراسة تنوعها الوراثي وتحديد درجة القرابة الوراثية بينها وذلك باستخدام تقنية ISSR.
Ecological and morphological characterization and geographical distribution of caper plant, Capparis spinosa L. samples collected from various parts of Syria were investigated during 2010-2014. The antioxidant contents of caper samples were analyzed and the genetic diversity and genetic relationship among the samples were determined using the ISSR technique
References used
Ames,B.N.,M.K.Shigenagaand T.M.Hagen.1993. Oxidants, antioxidants,and the degenerative diseases of aging. Proceedings of the National Academy of Sciences.90:2:7915–7922
Andallu, B.andN.Varadacharyulu.2003.Antioxidant role of mulberry (Morus indica L. cv. Anantha)leaves in streptozotocin-diabetic rats.Clinica Chimica Acta.338:6:3-10
Antunes,L.E.C.,J.Duarte,F.and C.M.Souza.2003. Conservação pós-colheita de frutos de amoreira-preta. Pesquisa Agropecuária Brasileira.38 3:413-419.
Arts,I.C.W.andP.C.H.Hollman.2005. Polyphenols and disease risk in epidemiologic studies.The American. Journal of Clinical Nutritio.81:1:17-25
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Research was carried out in (24) locations where two subspecies Capparis spinosa
were recorded in Syria . The subspecie C. spinosa subsp. rupestris was found in seven
locations, mostly in the southern region of the environment, while he found the S
yrian C.
spinosa subsp. spinosa in seventeen of the study sites in Syria. Chemical analysis showed
that, the soils of all location were not salty and pH ranged between (6.9-8.4), Soils texture
was clayey and clay loam in most locations. According dependant on equation of
Emberger, it was found that distribution of subspecies Capparis spinosa were located in a
wide range from bioclimatic very dry to semi- humid zone, through with dry, semi-dry,
and Hyper humid zones.
This present study was conducted to develop a detailed in vitro propagation
system for the medicinal shrub Capparis spinosa L.
Single nodes with one bud and a small part of stem of 1-1.5 cm long were
used as initial explants which were collected f
rom a shrubs grown under field
conditions at Damascus suburb., (Doumar). Explants were surface-disinfected
by 70% Ethanol for 1 min., followed by immersion in Sodium Hypochlorite or
HgCl2 for different periods and concentrations with 1 drop of Tween 20 for 100
ml disinfectant solution, where after, they were placed onto MS basal medium
containing a combination of growth regulators at different concentrations (BA
at 4.44 or 8.88 μM) each with IBA 0.49 μM. Cultures were incubated in the
growth room at 23±1 c and light intensity of 3000 lux at the cultures level.
Multiplication rate of 25.17-fold from one explant was achieved every 4 weeks
on the optimal MS medium (MS+8.88μM BA+0.49μM IBA).
The described method has potential to produce large numbers of plantlets
within a short period of time to expand its cultivation for medicinal uses.
Samples of Capparis spinosa plant were collected from 6 sites in Aleppo and Lattakia
provinces. The genetic variations were studied using the AFLP technique in order to determine the
genotypes of the studied types using 3 primers, which showed ampl
ification. Statistical analyses
were conducted using the dissimilarity coefficient and genetic diversity coefficient. The A.C.P. and
the statistical -F were calculated, and the similarity dendrogram was constructed. The results
showed the following:
The presence of a certain number of specific alleles (descriminates) for each province.
The presence of genetic and reproductive isolation deterrent to gene flow between the two
provinces.
The heterozygoty average was a little higher in Lattakia (0.486) than in Aleppo (0.481). The
mean of genetic diversity coefficient of primers and individuals was a little higher in Aleppo
(0.677) than in Lattakia (0.653). The population mean was very close: (0.759) in Aleppo and
(0.760) in Lattakia. This may be explained on the basis of silent and specific alleles due to deletion
mutations, different selective effects as well as the reproduction system in the two cities.
The greatest genetic distance in Aleppo was (0.381) noted between (Al Dahea and Al Shekh
saaed), and in Lattakia (0.38) noted between (Al Amroniah and Jabla). However, the greatest
genetic similarity in Aleppo was (0.637) noted between (Al Shekh Saaed and Turkman Bareh), and
in Lattakia (0.675) noted between (Wata Deirzenon and Jabla). The variation between these values
was graduated.
The study of statistical –F showed the effect of the similarity factor in some populations.
This was more significant in Aleppo than in Lattakia, which indicates that the reproduction system
is more closed in Aleppo, and this refers as well to inbreeding or self pollination which showed
high genetic variations in these populations when compared with Lattakia.
The results of this study helped in determining primers that can be used as molecular
markers in a breeding program for Capparis spinosa as a medicinal plant. This technique showed
high efficiency in studying the similarity relationships between these two cities.
The research aimed to evaluate the genetic diversity and the genetic
relationship between 16 Hordeum vulgare genotypes (variaties and new lines),
using SSR markers. 32 primer pairs were used in the analysis, five of them
amplified primers monomorp
hic alleles, while the remaining primers detected
different alleles. A total of 126 polymorphic alleles were revealed by the 27
primer pairs. The number of different alleles detected on alocus ranged from 2
to 11with a mean of 4.66 alleles per locus. The polymorphic information content
(PIC) values ranged from 0.21 to 0.88 with a mean value of 0.49, and the gene
diversity ranged from 0.23 to 0.89 with a mean value of 0.53.The dendrogram
was established using SSR data clustered into three major groups. The smallest
genetic relationship was observed between line39 and line38 . The results proved
the efficiency of SSR markers in the estimation of genetic diversity and in the
clustering of the barley genotypes in Syria.
In the present study¸ twelve parameters of Capparis spinosa were studied which are: plant length¸
number of main branches¸ number of leaf doubles, leaf length, leaf width, leaf area¸ number of budes,
number of flowers, number of fruits, weight of t
he fruits, number of seeds, weight of seeds. Statistical
analysis have been done using mean¸ variation¸ A.F.C.
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